ALEXIS Biochemicals: Monoclonal Antibody to Microcystin-LR (MC10E7)

ALX-804-320

Revised 19-Apr-07

Monoclonal Antibody to Microcystin-LR (MC10E7)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Microcystins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-320-C200		200 µg	210.00 USD

Product Specification

CLONE:	MC10E7
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with 200µl PBS containing sodium azide.
IMMUNOGEN:	Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin.
SPECIFICITY:	Recognizes all 4-Arg microcystins.
APPLICATION:	ELISA (1:10'000-1:100'000) Microcystin concentrations can be measured in the range of ~0.006µg/l to 0.3µg/l. The ELISA sensitivity is about 1'000 times higher compared to UV detection.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Storage at 4°C will decrease antibody activity and must be avoided.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Description

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC₅₀ values for microcystin-LR of 0.06µg/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 µg/l. All microcystin variants containing an arginine at position 4 show similar IC₅₀ values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x10¹⁰ l/mol.
The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1µg/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.

Product Specific Literature References

Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck, et al.; Anal. Chim. Acta 441, 1 (2001)
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001) Abstract

General Information

Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.