ALEXIS Biochemicals: Monoclonal Antibody to Microcystins (Adda specific) (AD4G2)

ALX-804-585

Revised 12-Apr-07

Monoclonal Antibody to Microcystins (Adda specific) (AD4G2)
SYNONYMS	anti-Microcystins (Adda specific) (AD4G2) MAb
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Microcystins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-585-C100		100 µg	190.00 USD

Product Specification

CLONE:	AD4G2
ISOTYPE:	Mouse IgG1
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with 100µl PBS containing sodium azide.
IMMUNOGEN:	N-Acetyl-Adda-KLH and N-Acetyl-Adda-BSA.
SPECIFICITY:	Recognizes all microcystins.
APPLICATION:	ELISA (1:10’000) Microcystins can be measured at a detection limit of ~0.03µg/l.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
POSITIVE CONTROL:	Products No. ALX-350-012, ALX-350-043, ALX-350-080 and ALX-350-081.

Product Description

With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody AD4G2, IC₅₀ values for microcystin-LR of 0.06 µg/l have been obtained. The provisional guideline value proposed by the WHO is 1µg/l for drinking water. The detection limit for microcystin-LR is 0.07µg/l. All microcystin variants show similar IC₅₀ values and detection limits. No microcystins are known, that are not recognized by this antibody. Microcystin-LR spiked water samples in the concentration range between 0.1 and 1µg/l were measured and a mean recovery of 113 ± 23% was found. The antibody is well suited for the sensitive analysis of all microcystins in drinking as well as surface water. Due to the very even cross-reactivity pattern of the antibody, a calibration with one microcystin (preferably MC-LR) (Prod. No. ALX-350-012) is sufficient for the quantitative determination of a sum concentration of all microcystins contained in a a sample.
For research purposes only, not for commercial ELISA development.

Product Specific Literature References

Generic microcystin immunoassay based on monoclonal antibodies against Adda: A. Zeck, et al.; Analyst. 126, 2002 (2001) Abstract
Multidimensional biochemical detection of microcystins in liquid chromatography: A. Zeck, et al.; Anal. Chem. 73, 5509 (2001) Abstract
Highly sensitive immunoassay based on a monoclonal antibody specific for [4-arginine]microcystins: A. Zeck et al.; Anal.Chim. Acta 441, 1 (2001)
Development of a direct competitive microcystin immunoassay of broad specificity: M.G. Weller, et al.; Anal. Sci. 17, 1445 (2001) Abstract

General Information

Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals.
Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 amino acid 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-amino acids.

ALX-350-043	Microcystin-RR
ALX-350-012	Microcystin-LR
ALX-350-080	Microcystin-LW
ALX-350-081	Microcystin-LF