ALEXIS Biochemicals: Monoclonal Antibody to Nitrotyrosine [Affinity Sorbent] (CC22.8C7)

ALX-804-416

Revised 08-Aug-05

Monoclonal Antibody to Nitrotyrosine [Affinity Sorbent] (CC22.8C7)
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	Nitrotyrosine/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-416-C200		200 µg	403.00 USD

Product Specification

CLONE:	CC22.8C7
ISOTYPE:	Mouse IgG
FORMULATION:	200µg of anti-Nitrotyrosine MAb conjugated to 200µl of protein A agarose. Supplied as a 50% slurry in TBS, pH 8.0, containing 0.02% sodium azide.
IMMUNOGEN:	Peroxynitrite treated KLH (keyhole limpet hemocyanin).
SPECIFICITY:	Recognizes nitrotyrosine-containing proteins.
APPLICATION:	Immunoprecipitation.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year when stored at +4°C.

General Information

BACKGROUND/TECHNICAL INFORMATION

Typical procedure for Immunoprecipitation:

1. Dilute the protein sample (cell lysate, tissues homogenate, plasma, etc.) to ~1mg/ml in TBS or PBS in a
     microcentrifuge tube.
2. Add 20-40µl of gel slurry to 0.5-1ml (0.5-1mg protein) and incubate 1-2 hours at room temperature or
     4°C overnight with occasional mixing or gentle rocking.
3. Collect the beads by brief centrifugation (<1 minute) in a microcentrifuge. Carefully drain or pipet off the
     supernatant and wash the beads 3 times with TBS or PBS.
4. Resuspend the beads in 25-50µl of 1X Laemmli sample buffer and boil for 5 minutes. (Alternatively the
     samples can be frozen for later use).
5. Briefly centrifuge the sample to pellet the beads. The supernatant can now be directly used for
     SDS-PAGE.

Further Categories Containing This Product:

Monoclonal Antibodies

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