ALEXIS Biochemicals: Monoclonal Antibody to PAG/Cbp (human) (MEM-255)

ALX-804-437

Revised 13-Feb-07

Monoclonal Antibody to PAG/Cbp (human) (MEM-255)
SYNONYMS	anti-Phosphoprotein Associated with GEMS (human) MAb (MEM-255) anti-Csk-binding Protein (human) MAb (MEM-255)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Adaptor Proteins in T Cell Signal Transduction / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-437-C100		100 µg	270.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	MEM-255
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:	Recombinant human PAG/Cbp (phosphoprotein associated with GEMS/Csk-binding protein) intracellular domain (aa 97-432).
SPECIFICITY:	Recognizes human PAG/Cbp. Does not cross-react with mouse, rat and bovine PAG/Cbp. Detects a band of ~80kDa by Western blot (see below).
APPLICATION:	Flow Cytometry (Intracellular staining, permeabilization required (2µg/ml)) Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation: T. Brdicka, et al.; J. Exp. Med. 191, 1591 (2000) Abstract; Full Text
Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton: N. Brdickova, et al.; FEBS Lett. 507, 133 (2001) Abstract; Full Text
Release from tonic inhibition of T cell activation through transient displacement of C-terminal Src kinase (Csk) from lipid rafts: K.M. Torgersen, et al.; J. Biol. Chem. 276, 29313 (2001) Abstract; Full Text
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling: T. Vang, et al.; J. Biol. Chem. 278, 17597 (2003) Abstract; Full Text
Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor: D. Davidson, et al.; Mol. Cell Biol. 23, 2017 (2003) Abstract
Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes: M. Baumgartner, et al.; Blood 101, 1874 (2003) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: PAG/Cbp is an ubiquitously expressed ~46kDa transmembrane adapter protein present in microdomains (rafts), which however migrates on SDS-PAGE gels anomalously as an ~80kDa molecule.

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