ALEXIS Biochemicals: Monoclonal Antibody to Perforin (mouse) (CB5.4)

ALX-804-057

Revised 03-Apr-06

Monoclonal Antibody to Perforin (mouse) (CB5.4)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Perforin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-057-C100	Purified	100 µg	290.00 USD
ALX-804-057F-T100	FITC	100 tests	350.00 USD
ALX-804-057TD-T100	ATTO 488	100 tests	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse
CLONE:	CB5.4
ISOTYPE:	Rat IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant mouse perforin (aa 98-534).
SPECIFICITY:	Recognizes mouse perforin (region aa 402-534).
APPLICATION:	CB5.4-Purified: Immunocytochemistry Immunohistochemistry (frozen sections, paraffin sections) Immunoprecipitation Western Blot CB5.4-ATTO 488: Immunocytochemistry CB5.4-FITC: Immunocytochemistry
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at +4°C.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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Product Description

New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90’000).

Product Specific Literature References

The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993) Abstract
Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller & J. Tschopp; J. Immunol. 153, 2470 (1994) Abstract
Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002) Abstract; Full Text
Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION	CB5.4-FITC: 1 test means: 1µl of a 1mg/ml stock solution were used to stain 200’000 cells in a sample volume of 50µl.
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002) Abstract
Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004) Abstract

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