ALEXIS Biochemicals: Monoclonal Antibody to Phosphoserine (1C8)

ALX-804-162

Revised 12-Feb-07

Monoclonal Antibody to Phosphoserine (1C8)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phospho-specific Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-162-C100		100 µg	453.00 USD

Product Specification

CLONE:	1C8
ISOTYPE:	Mouse IgM
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN:	Phosphoserine containing peptides.
SPECIFICITY:	Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
APPLICATION:	Immunoprecipitation Western Blot (1µg/ml for HRPO/ECL detection). Recommended blocking buffer BPPT: 2x PBS containing 3% (w/v) BSA, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution!
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-012)

Product Specific Literature References

Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997) Abstract; Full Text
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998) Abstract
Insulin-mimetic signalling of synthetic phosphoinositolglycans in isolated rat adipocytes: W. Frick, et al.; Biochem. J. 336, 163 (1998) Abstract; Full Text
Functional regulation of metabotropic glutamate receptor type 1c: a role for phosphorylation in the desensitization of the receptor: F. Ciruela, et al.; FEBS Lett. 462, 278 (1999) Abstract; Full Text
Light regulation of protein phosphorylation in Blepharisma japonicum: H. Fabzak et al.; Acta Protozool. 40, 311 (2001)
Regulation of the ligand binding activity of the human very low density lipoprotein receptor by protein kinase C-dependent phosphorylation: R. Sakthivel, et al.; J. Biol. Chem. 276, 555 (2001) Abstract; Full Text
Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites: S. Suer, et al.; Eur. J. Biochem. 268, 2099 (2001) Abstract; Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt, et al.; Proteomics 3, 1637 (2003) Abstract