ALEXIS Biochemicals: Monoclonal Antibody to Phosphoserine (4A3)

ALX-804-163

Revised 27-Mar-07

Monoclonal Antibody to Phosphoserine (4A3)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phospho-specific Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-163-C100		100 µg	453.00 USD

Product Specification

CLONE:	4A3
ISOTYPE:	Mouse IgM
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Phosphoserine containing peptides.
SPECIFICITY:	Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.). Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
APPLICATION:	Immunoprecipitation Western Blot (1µg/ml for HRPO/ECL detection. Recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-012)

Product Specific Literature References

Regulatory phosphorylation of the Na+/K+-ATPase from mammalian kidneys and Xenopus oocytes by protein kinases: L. A. Vasilets; Ann. N.Y. Acad. Sci. 834, 585 (1997)
Characterization and purification of human corneodesmosin, an epidermal basic glycoprotein associated with corneocyte-specific modified desmosomes: M. Simon, et al.; J. Biol. Chem. 272, 31770 (1997) Abstract; Full Text
Insulin signaling in the yeast Saccharomyces cerevisiae. 3. Induction of protein phosphorylation by human insulin: G. Muller, et al.; Biochemistry 37, 8705 (1998) Abstract
The neuropeptide proctolin induces phosphorylation of a 30 kDa protein associated with the thin filament in crustacean muscle: B. Brustle, et al.; J. Exp. Biol. 204, 2627 (2001) Abstract; Full Text
Towards a phosphoproteome map of Corynebacterium glutamicum: A.K. Bendt et al.; Proteomics 3, 1637 (2003) Abstract