ALEXIS Biochemicals: Monoclonal Antibody to Ski (G8)

ALX-804-221

Revised 22-Mar-06

Monoclonal Antibody to Ski (G8)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-221-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
CLONE:	G8
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant v-Ski.
SPECIFICITY:	Recognizes cellular and hyperphosphorylated human, mouse, rat, chicken and Xenopus Ski. Detects bands of ~95kDa (Ski) and bands of up to ~115kDa (hyperphosphorylated Ski) by Western blot.
APPLICATION:	Immunocytochemistry (1-10µg/ml) Immunoprecipitation (1-4µg per sample) Western Blot (1-5µg/ml)
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-20°C

Product Images

Please click on thumbnails to enlarge.

Product Specific Literature References

Association of specific DNA binding and transcriptional repression with the transforming and myogenic activities of c-Ski: R. Nicol, et al.; Cell Growth Differ. 10, 243 (1999) Abstract; Full Text
Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents: S.B. Cohen, et al.; Nucl. Acids Res. 27, 1006 (1999) Abstract; Full Text

General Information

Ski (v-ski) was discovered as a viral oncogene from the genome of avian Sloan-Kettering retrovirus. Overexpression of both v-ski and cellular homolog c-ski in avian fibroblasts leads to anchorage-independent growth and morphological transformation of chicken embryo fibroblasts. Overexpression of c-ski has also been observed in several types of human cancer cells. New results show that Ski associates with Smads2, 3 and 4 proteins in response to TGF-β stimulation. Interactions between Ski and Smad2, 3 and 4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. Ski is also required for transcriptional repression mediated by Mad involved in negative regulation of cellular proliferation and plays a role in muscle differentiation.

General Literature References

The ski oncogene: P. Sutrave & S.H. Hughes; Oncogene 6, 353 (1991), (Review) Abstract
The Ski oncoprotein interacts with the Smad proteins to repress TGFbeta signaling: K. Luo, et al.; Genes Dev. 13, 2196 (1999) Abstract; Full Text
SnoN and Ski protooncoproteins are rapidly degraded in response to transforming growth factor beta signaling: Y. Sun, et al.; PNAS 96, 12442 (1999) Abstract; Full Text
Viral ski inhibits retinoblastoma protein (Rb)-mediated transcriptional repression in a dominant negative fashion : F. Tokitou, et al.; J. Biol. Chem. 274, 4485 (1999) Abstract; Full Text
Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor : T. Nomura, et al.; Genes Dev. 13, 412 (1999) Abstract; Full Text
c-Ski acts as a transcriptional Co-repressor in transforming growth factor-beta signaling through interaction with smads : S. Akiyoshi, et al. ; J. Biol. Chem. 274, 35269 (1999) Abstract; Full Text
Interaction of the Ski oncoprotein with Smad3 regulates TGF-beta signaling: Y. Sun, et al.; Mol. Cell 4, 499 (1999) Abstract

Further Categories Containing This Product:

Transcription Factors Other Products • Monoclonal Antibodies

another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.