ALEXIS Biochemicals: Monoclonal Antibody to SnoN (1G1)

ALX-804-360

Revised 05-Oct-05

Monoclonal Antibody to SnoN (1G1)
SYNONYMS	anti-Ski-related Novel Protein Non-Alu-containing MAb (1G1)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-360-C100		100 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Others
CLONE:	1G1
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant SnoN (Ski-related novel protein non-Alu-containing).
SPECIFICITY:	Recognizes human and chicken SnoN. Detects a band of ~80kDa by Western blot.
APPLICATION:	Immunoprecipitation (5µg/sample) Western Blot (1-5µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 2 years after receipt when stored at -20°C.

Product Description

Ski [1] and SnoN [2] are oncoproteins and transcription factors that form both homodimers and heterodimers [3]. Both have the ability to transform avian fibroblasts and induce muscle differentiation [4,5]. They recognize a specific DNA binding site (GTCTAGAC) through which they repress transcription [6]. Ski and SnoN are components of a macromolecular complex containing the co-repressor N-CoR/SMRT, mSin3 and histone deacetylase. Ski and SnoN negatively regulate TGF-β signalling by recruiting this complex to Smads [7]. Interactions between Ski and Smad2, -3 and -4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. For a review see [8].

Product Specific Literature References

[1] Generation of transforming viruses in cultures of chicken fibroblasts infected with an avian leukosis virus: E. Stavnezer, et al.; J. Virol. 39, 920 (1981)
[2] Isolation of human cDNA clones of ski and the ski-related gene, sno: N. Nomura, et al.; Nucl. Acids Res. 17, 5489 (1989) Abstract
[3] Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents: S.B. Cohen, et al.; Nucl. Acids Res. 27, 1006 (1999)
[4] The ski oncogene induces muscle differentiation in quail embryo cells: C. Colmenares & E. Stavnezer; Cell 59, 293 (1989)
[5] Sequence and biological activity of chicken snoN cDNA clones: P.L. Boyer, et al.; Oncogene 8, 457 (1993)
[6] A domain necessary for the transforming activity of SnoN is required for specific DNA binding, transcriptional repression and interaction with TAF(II)110: S.B. Cohen, et al.; Oncogene 17, 2505 (1998) Abstract
[7] Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor:: T. Nomura, et al.; Genes Dev. 13, 412 (1999) Abstract
[8] Ski/Sno and TGF-beta signaling: X. Liu, et al.; Cytokine Growth Factor Rev. 12, 1 (2001), (Review) Abstract

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Transcription Factors Other Products • Monoclonal Antibodies

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