ALEXIS Biochemicals: Monoclonal Antibody to TNF-α (human) (TNF-D)

ALX-804-199

Revised 18-Sep-07

Monoclonal Antibody to TNF-α (human) (TNF-D)
SYNONYMS	anti-Tumor Necrosis Factor-α (human) MAb (TNF-D) anti-TNFSF 2 (human) MAb (TNF-D)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	TNF-alpha

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-199-C100		100 µg	259.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	TNF-D
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In PBS.
IMMUNOGEN:	Recombinant human TNF-α (tumor necrosis factor-α).
SPECIFICITY:	Recognizes human TNF-α. Does not cross-react with mouse TNF-α or human or mouse TNF-β.
APPLICATION:	ELISA: Use as capture or detection antibody in sandwich ELISA. Flow Cytometry: Suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes. Functional Application: Selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity. Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After opening aliquot contents and freeze at -20°C.

Product Specific Literature References

Monoclonal antibodies to human tumor necrosis factors alpha and beta: application for affinity purification, immunoassays, and as structural probes: T.S. Bringman & B.B. Aggarwal; Hybridoma 6, 489 (1987) Abstract

Further Categories Containing This Product:

Monoclonal Antibodies

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