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ALX-804-200 Revised 04-Apr-06
Monoclonal Antibody to TNF-R1 (human) (H398)
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SYNONYMS anti-TNF-R 60kDa (human) MAb (H398)
anti-p55 (human) MAb (H398)
anti-CD120a (human) MAb (H398)
anti-TNFRSF 1A (human) MAb (H398)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R1
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-200-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: H398
ISOTYPE: Mouse IgG2a
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Human TNF-R1.
SPECIFICITY: Recognizes membrane-bound and soluble human TNF-R1. Does not cross-react with mouse TNF-R1.
APPLICATION: Flow Cytometry
Immunohistochemistry (frozen sections): Stains acetone-fixed cryostat sections or cell smears. Recommended for H398 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. H398 may be used at a concentration of 1-0.5mg/ml for cryostat sections.
Not recommended for paraffin sections.
Functional Application: Blocks TNF-R signalling. 10µg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%). Furthermore, 10µg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%).
TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells. Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses: B. Thoma, et al.; J. Exp. Med. 172, 1019 (1990) Abstract
Monoclonal antibodies to soluble human TNF receptor (TNF binding protein) enhance its ability to block TNF toxicity: G.R. Adolf & B. Fruhbeis; Cytokine 4, 180 (1992) Abstract
Inhibition of tumor necrosis factor (TNF)-mediated NF-kappa B activation by selective blockade of the human 55-kDa TNF receptor: G. Kruppa, et al.; J. Immunol. 148, 3152 (1992) Abstract
TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis: M. Grell, et al.; Lymphokine Cytokine Res. 12, 143 (1993) Abstract
Cytostatic and cytotoxic effects of tumor necrosis factor alpha on MCF-7 human breast tumor cells are differently inhibited by glucocorticoid hormones: M.C. Pagliacci, et al.; Lymphokine Cytokine Res. 12, 439 (1993) Abstract
Lymphotoxin acts as an autocrine growth factor for Epstein-Barr virus-transformed B cells and differentiated Burkitt lymphoma cell lines: D.L. Gibbons, et al.; Eur. J. Immunol. 24, 1879 (1994) Abstract
TNF-induced superoxide anion production in adherent human neutrophils involves both the p55 and p75 TNF receptor: J. Richter, et al.; J. Immunol. 154, 4142 (1995) Abstract
Gastrin secretion from primary cultures of rabbit antral G cells: stimulation by inflammatory cytokines: N. Weigert, et al.; Gastroenterology 110, 147 (1996) Abstract
1alpha,25-dihydroxyvitamin D3 induces sphingomyelin hydrolysis in HaCaT cells via tumor necrosis factor alpha: C.C. Geilen, et al.; J. Biol. Chem. 272, 8997 (1997) Abstract; Full Text
Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway: K.B. Choi, et al.; J. Biol. Chem. 273, 20185 (1998) Abstract; Full Text
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism: D. Tang, et al.; J. Biol. Chem. 274, 7245 (1999) Abstract; Full Text
Interferon-gamma rescues TNF-alpha-induced apoptosis mediated by up-regulation of TNFR2 on EoL-1 cells: T. Horie, et al.; Exp. Hematol. 27, 512 (1999) Abstract
Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand: G. Lu, et al.; J. Immunol. 168, 1831 (2002) Abstract
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 

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