ALEXIS Biochemicals: Monoclonal Antibody to Toll-like Receptor 4 (human) (HTA125)

ALX-804-419

Revised 09-Jul-07

Monoclonal Antibody to Toll-like Receptor 4 (human) (HTA125)
SYNONYMS	anti-TLR4 (human) MAb (HTA125) anti-CD284 (human) MAb (HTA125)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-419-C100		100 µg	270.00 USD
ALX-804-419PF-C100	Preservative Free	100 µg	270.00 USD
ALX-804-419F-T100	FITC	100 tests	300.00 USD
ALX-804-419TD-T100	ATTO 488	100 tests	340.00 USD
ALX-804-419TS-T100	ATTO 647N	100 tests	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	HTA125
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide. HTA125 (preservative free): Liquid. In PBS.
IMMUNOGEN:	Ba/F3 cell line expressing human TLR4 (Toll-like receptor 4).
SPECIFICITY:	Recognizes human TLR4 cell surface antigen.
APPLICATION:	HTA125: Flow Cytometry: Incubate at 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunoprecipitation HTA125 (preservative free): Flow Cytometry: Incubate at 37°C (1:10-1:100) and then on ice with GAM-FITC. Immunoprecipitation Functional Application (blocks activation of monocytes with LPS; use preservative free formulation). HTA125-ATTO 488: Flow Cytometry (1:100) HTA125-ATTO 647N: Flow Cytometry HTA125-FITC: Flow Cytometry Immunoprecipitation Note: Not suitable for Western blot (does not detect denatured protein).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.

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Product Specific Literature References

MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4: R. Shimazu, et al.; J. Exp. Med. 189, 1777 (1999) Abstract; Full Text
Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2: S. Akashi, et al.; BBRC 268, 172 (2000) Abstract
Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-κB: Q. Jiang, et al.; J. Immunol. 165, 3541 (2000) Abstract; Full Text
Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts: K. Tabeta, et al.; Infect. Immun. 68, 3731 (2000) Abstract; Full Text
Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14: E. Ellingsen, et al.; Med. Sci. Monitor 8, BR149 (2002) Abstract; Full Text
Lipopolysaccharide rapidly traffics to and from the Golgi apparatus with the toll-like receptor 4-MD-2-CD14 complex in a process that is distinct from the initiation of signal transduction: E. Latz, et al.; J. Biol. Chem. 277, 47834 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl.
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Antibodies • Monoclonal Antibodies

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