ALEXIS Biochemicals: Monoclonal Antibody to VASP (IE273)

ALX-804-177

Revised 13-Nov-07

Monoclonal Antibody to VASP (IE273)
SYNONYMS	anti-Vasodilator Stimulated Phosphoprotein MAb (IE273)
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	VASP/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-177-C050		50 µg	295.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Pig Others
CLONE:	IE273
ISOTYPE:	Mouse IgG1
CONCENTRATION:	500µg/ml
FORMULATION:	Liquid. In PBS containing 0.01% sodium azide.
IMMUNOGEN:	Purified human VASP (vasodilator stimulated phosphoprotein).
SPECIFICITY:	Recognizes both the 46kDa (Ser¹⁵⁷ dephospho-) and 50kDa (Ser¹⁵⁷ phospho-) form of human, rabbit, pig and bovine VASP.
APPLICATION:	Immunocytochemistry (1:100-1:500) Immunoprecipitation Western Blot (1:2'000-1:5'000) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt). Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.

Product Specific Literature References

Monoclonal antibodies against the focal adhesion protein VASP revealing epitopes involved in the interaction with two VASP binding proteins and VASP phosphorylation: Abel, et al.; Eur. J. Cell Biol. 69 (Suppl. 42), 39a (1996) Abstract
A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family: K. Niebuhr, et al.; EMBO J. 16, 5433 (1997) Abstract
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Sitmulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001) Abstract; Full Text

General Information

VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif,
b) a more divergent proline-rich central domain (which is responsible for profilin binding), and
c) a conserved C-terminal EVH2 domain.

Particularly high VASP levels are present in platelets, although VASP is expressed in a wide variety of cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.

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