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ALX-804-402 Revised 07-Nov-06
Monoclonal Antibody to cGMP-activated Protein Kinase Substrate (phosphorylated) (7H1)
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SYNONYMS anti-Protein Kinase G Substrate (phosphorylated) MAb (7H1)
anti-PKG Substrate (phosphorylated) MAb (7H1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKG Substrates
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-402-C100   100 µg Inquire
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 7H1
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 2x PBS, containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic phosphopeptide corresponding to PKG (protein kinase G) substrate consensus phosphorylation site of activated PKG substrate.
SPECIFICITY: Recognizes VpSK motif of activated PKG substrate in a broad range of species.
APPLICATION: ELISA (non-radioactive quantification of phospho-peptides in kinase/phosphatase assays; use at 0.1-1µg/ml).
Note: Not suitable for Western blot!
Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
 
 

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