ALEXIS Biochemicals: Monoclonal Antibody to ERK1/2 (phosphorylated) (12D4)

ALX-804-061

Revised 24-Jun-08

Monoclonal Antibody to ERK1/2 (phosphorylated) (12D4)
SYNONYMS	anti-MAPK2/3 (phosphorylated) MAb (12D4) anti-MAP Kinase 2/3 (phosphorylated) MAb (12D4)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	ERK1 & ERK2 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-061-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	12D4
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2.
SPECIFICITY:	Recognizes the pThr-Glu-pTyr motif of activated human, mouse, rat and dog ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38.
APPLICATION:	Immunocytochemistry (1-10µg/ml) Immunohistochemistry Immunoprecipitation (1-10µg per 10⁶ pervanadate-treated A431 or HepG2 cells) Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-025).

Product Specific Literature References

IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005) Abstract; Full Text

Further Categories Containing This Product:

Phospho-specific Antibodies • Monoclonal Antibodies

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