ALEXIS Biochemicals: NF-κB (p50) (human) (recombinant) (His)

ALX-201-285

Revised 09-Oct-08

NF-κB (p50) (human) (recombinant) (His)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	NF-kB Transcription Factors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-285-C002		2 µg	280.00 USD

Product Specification

SOURCE/HOST:	Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION:	0.2mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.5, containing 125mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY:	~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.

Product Specific Literature References

Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P19838: NF-κB (human) (p50 subunit)
AfCS Signalling Gateway link A002937: NF-κB (mouse) (p50 subunit)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

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