ALEXIS Biochemicals: NMNAT1 (human) (recombinant) (His)

ALX-201-237

Revised 12-Aug-08

NMNAT1 (human) (recombinant) (His)
SYNONYMS	NMN Adenylyltransferase 1 (human) (recombinant) (His) Nicotinamide Mononucleotide Adenylyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	NAD+ Metabolism / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-237-C100		100 µg	390.00 USD

Product Specification

MW:	33.3kDa (monomer)
EC:	2.7.7.1
SOURCE/HOST:	Produced in E. coli. Full length human NMNAT1 is fused at the N-terminus to a His-tag.
CONCENTRATION:	~1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
SPECIFIC ACTIVITY:	10-20U/mg
APPLICATION:	Well suited for the synthesis of NAD due to high specific activity (10-20U/mg) and high substrate selectivity compared to NMNAT3 (human) (recombinant) (Prod. No. ALX-201-238). Note: When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min. before the assay.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 3 months after receipt when stored at -20°C. Unstable in solutions containing less than 250mM salt.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem. 276, 406 (2001) Abstract; Full Text
Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett. 492, 95 (2001) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q9HAN9: NMNAT1 (human)

ALX-201-238	NMNAT3 (human) (recombinant)
ALX-201-429	NMNAT1 (human) (recombinant) (His)