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ALX-620-054
Revised 11-Oct-06
4AF DA
SYNONYMS
4-Aminofluorescein diacetate
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-054-M001
1 mg
136.00 USD
Product Specification
FORMULA:
C
24
H
17
NO
7
MW:
431.4
PURITY:
≥99% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light. Keep under inert gas.
Product Description
Negative control compound for the fluorescent probes DAF-2 (Prod. No.
ALX-620-052
) and DAF-2 DA (Prod. No.
ALX-620-056
).
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA:
H. Kojima, et al.; Neuroreport
9
, 3345 (1998)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-029
Revised 05-May-08
ABD-F
SYNONYMS
4-Fluoro-7-sulfoamoylbenzofurazan
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-620-029-M010
10 mg
95.00 USD
ALX-620-029-M050
50 mg
320.00 USD
Product Specification
FORMULA:
C
6
H
4
FN
3
O
3
S
MW:
217.2
CAS NUMBER:
91366-65-3
PURITY:
≥99%
APPEARANCE:
White to yellow powder.
SOLUBILITY:
Soluble in acetonitrile.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light and moisture.
Product Description
Highly reactive fluorogenic reagent for labelling thiol compounds. Selectively labels protein cysteine residues without side chain modification or N-terminal blockage. Potent vasorelaxant.
Product Specific Literature References
New Fluorogenic Reagent Having Halogenobenzofurazan Structure for Thiols: 4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole:
T. Toyo'oka and K. Imai; Anal. Chem.
56
, 2461 (1984)
Isolation and characterization of cysteine-containing regions of proteins using 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole and high- performance liquid chromatography:
T. Toyo'oka and K. Imai; Anal. Chem.
57
, 1931 (1985)
Amino acid composition analysis of minute amounts of cysteine- containing proteins using 4-(aminosulfonyl)-7-fluoro-2,1,3- benzoxadiazole and 4-fluoro-7-nitro-2,1,3-benzoxadiazole in combination with HPLC:
T. Toyo'oka, et al.; Biomed. Chromatogr.
1
, 15 (1986)
Abstract
Fluorometric assay of thiols with fluorobenzoxadiazoles:
K. Imai and T. Toyo'oka; Meth. Enzymol.
143
, 67 (1987)
Abstract
ALX-610-028
Revised 17-Jan-06
7-Amino-4-methylcoumarin
SYNONYMS
AMC
Coumarin 120
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-028-M010
10 mg
20.00 USD
Product Specification
FORMULA:
C
10
H
9
NO
2
MW:
175.2
CAS NUMBER:
26093-31-2
PURITY:
≥98%
APPEARANCE:
Off-white to yellow powder.
SOLUBILITY:
Soluble in dimethylformamide, acetone or DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Keep cool and dry. Keep under inert gas.
Product Description
Fluorogenic chromophore. Ex(max): 380nm; Em(max) 460nm. Reference compound.
ALX-610-027
Revised 29-Nov-04
7-Amino-4-trifluoromethylcoumarin
SYNONYMS
AFC
Coumarin 151
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-027-M010
10 mg
15.00 USD
Product Specification
FORMULA:
C
10
H
6
F
3
NO
2
MW:
229.2
CAS NUMBER:
53518-15-3
PURITY:
≥97%
APPEARANCE:
Off white to yellow powder.
SOLUBILITY:
Soluble in DMSO, DMF or methanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light. Keep under inert gas.
Product Description
Fluorogenic chromophore. Ex(max): 400nm; Em(max) 505nm. Reference compound.
ALX-620-075
Revised 25-Sep-08
APF
SYNONYMS
Aminophenyl fluorescein
2-[6-(4'-Amino)phenoxy-3
H
-xanthen-3-on-9-yl]benzoic acid
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Oxidative Stress Markers & Reagents / Related Products
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ALX-620-075-M001
1 mg
430.00 USD
Product Specification
FORMULA:
C
26
H
17
NO
5
MW:
423.4
CAS NUMBER:
359010-70-1
CONCENTRATION:
~5mM
PURITY:
≥98% (HPLC)
FORMULATION:
Liquid. In 0.47ml dimethyl formamide.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Prepare 500-5’000-fold dilution (~10-1µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store the dilutions.
HANDLING:
Keep under inert gas. Protect from light. After opening, prepare aliquots and store at +4°C.
HAZARD:
HARMFUL.
Product Images
Please click on thumbnails to enlarge.
Product Description
Fluorescent reagent (Ex(max): 490nm; Em(max): 515nm) for the detection of highly reactive oxygen species (hROS). Immediately reacts with hROS such as hydroxyl radical, peroxynitrite and hypochlorite, and the fluorescence intensity greatly increases. Use of APF together with HPF (Prod. No.
ALX-620-074
) also allows for specific detection of hypochlorite (
-
OCl) to elucidate reliable the roles of
-
OCl in biological systems such as neutrophils. In addition, peroxynitrite can be detected in distinction from nitric oxide and superoxide since APF does not react with nitric oxide, superoxide and hydrogen peroxide. Moreover, APF is resistant to light-induced autooxidation.
Not for sale in Japan.
Product Specific Literature References
Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species
:
K. Setsukinai, et al.; J. Biol. Chem.
278
, 3170 (2003)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Precautions for use:
1. Prepare the diluted solution just before use and use it up.
2. The dilution buffer should be adjusted to pH 7.0-7.5. Please note that bovine serum albumin (BSA) and phenol red might interfere with the fluorescence measurement.
Light-induced autoxidation (Figure 3)
After adding HPF or DCFH-DA (10µM), the cells were incubated for 30 min at 37°C in the dark. The fluorescence images were obtained with a confocal fluorescent microscope (excitation wavelength: 488nm, fluorescence emission wavelength: using a 515nm barrier filter). The cells were laser-irradiated again at 488nm for 10 sec and fluorescence images were obtained again (see Figure 3).
Fluorescence images of HPF- or APF-loaded neutrophils (Figure 4)
Neutrophils were obtained from porcine blood and suspended in Krebs-Ringer phosphate buffer (114mM NaCI, 4.6mM KCI, 2.4mM MgSO
4
, 1.0mM CaCI
2
, 15mM NaH
2
PO
4
/Na
2
HPO
4
, pH 7.4) and kept on ice until use. Then they were seeded onto a glass-bottomed dish. The cells were loaded with HPF or APF (10µM) by incubation for 30min at room temperature and then stimulated with PMA (including 2ng/ml; 0.1% DMF as a cosolvent). Fluorescence images were obtained with a confocal fluorescent microscope before and 10 min after the stimulation with PMA (excitation wavelength: 488nm, fluorescence emission wavelength: 505-550nm; using a 505-550nm barrier filter).
Detection of hROS in the HRP/H
2
O
2
system using HPF and APF (Figure 5)
Reaction timecourse
Fluorescence probe reagents (final 10µM; 0.1% DMF as a cosolvent) were added to sodium phosphate buffer (0.1M; pH 7.4) containing HRP (0.2µM). H
2
O
2
(final 1µM) was added at the time indicated by the arrow. The fluorescence intensities were measured at excitation wavelength of 490nm and fluorescence emission wavelength of 515nm. Relation between the amount of added H
2
O
2
and fluorescence increase in the HRP/H
2
O
2
system.
Relation between the amount of added H
2
O
2
and fluorescence increase in the HRP/H
2
O
2
system
Fluorescence probe reagents (final 10µM; 0.1% DMF as a cosolvent) were added to sodium phosphate buffer (0.1M; pH 7.4) containing HRP (0.2µM). The fluorescence intensities were measured at excitation wavelength of 490nm and fluorescence emission wavelength of 515nm.
Related Products
ALX-620-074
HPF
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
•
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-450-013
Revised 22-Feb-08
BAPTA . tetrapotassium salt
SYNONYMS
1,2-
bis
(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid . 4K
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calcium Chelators / Caged Calcium Chelators
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ALX-450-013-G001
1 g
98.00 USD
Product Specification
FORMULA:
C
22
H
20
N
2
O
10
. 4K
MW:
472.4 . 156.4
CAS NUMBER:
73630-08-7
PURITY:
≥97% (as anhydride)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light and moisture.
Product Description
Highly selective Ca
2+
chelating agent.
Product Specific Literature References
New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures:
R.Y. Tsien; Biochemistry
19
, 2396 (1980)
Abstract
Calcium-independent increase of transmitter release at frog end-plate by trinitrobenzene sulphonic acid:
H. Kijima & T. Tanabe; J. Physiol.
403
, 135 (1988)
Abstract
Transmitter release at frog end-plate loaded with a Ca2+-chelator, BAPTA: hypertonicity and erythrosin B augment the release independently of internal Ca2+:
N. Tanabe & H. Kijima; Neurosci. Lett.
92
, 52 (1988)
Abstract
Properties of neuroprotective cell-permeant Ca2+ chelators: effects on [Ca2+]i and glutamate neurotoxicity in vitro:
M. Tymianski, et al.; J. Neurophysiol.
72
, 1973 (1994)
Abstract
Chelation of intracellular Ca2+ inhibits murine keratinocyte differentiation in vitro:
L. Li, et al.; J. Cell Physiol.
163
, 105 (1995)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
•
Nuclear Envelope & Nuclear Transport
ALX-620-051
Revised 30-Jun-08
Bisbenzimide H33258 . trihydrochloride
SYNONYMS
HOE 33258
2'-4-(Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-
bis
-1
H
-benzimidazole . 3HCl
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-620-051-M010
10 mg
22.00 USD
ALX-620-051-M050
50 mg
35.00 USD
Product Specification
FORMULA:
C
25
H
24
N
6
O . 3HCl
MW:
424.4 . 109.4
CAS NUMBER:
23491-45-4
RTECS:
SM1140500
PURITY:
≥98% (TLC)
APPEARANCE:
Yellow to greenish powder.
SOLUBILITY:
Soluble in water (10mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
HAZARD:
MAY BE MUTAGENIC. HARMFUL.
IDENTITY:
Identity determined by IR and
1
H-NMR.
Product Description
Cell permeable fluorescent dye with low cytotoxicity. Useful for staining DNA, chromosomes and nuclei [1,2]. Can be used with chromomycin A
3
(Prod. No.
ALX-380-055
) to distinguish chromosomes by their total DNA concentration and their DNA base composition [3,4]. Binds to A:T pairs in DNA. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA [5]. Increases the rate of channel-mediated Ca
2+
efflux from junctional cytoplasmic reticulum vesicles [6]. Ex(max): 346nm; Em(max): 460nm.
Product Specific Literature References
[1]
Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis
:
S.A. Latt & G. Stetten; J. Histochem. Cytochem.
24
, 24 (1976)
Abstract
[2]
Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations:
K.H. Elstein & R.M. Zucker; Exp. Cell. Res.
211
, 322 (1994)
Abstract
[3]
Bacterial characterization by flow cytometry
:
M.A. Van Dilla, et al.; Science
220
, 620 (1983)
Abstract
[4]
Determination of guanine-plus-cytosine content of bacterial DNA by dual-laser flow cytometry
:
C.A. Sanders, et al.; J. Gen. Microbiol.
136
, 359 (1990)
Abstract
[5]
Controlling gene expression in living cells through small molecule-RNA interactions
:
G. Werstuck & M.R. Green; Science
282
, 296 (1998)
Abstract
[6]
Activation and inhibition of the sarcoplasmic reticulum Ca2+ channel by the polycationic dyes Hoechst 33342 and Hoechst 33258:
T.J. Beeler & K. Gable; J. Membr. Biol.
135
, 109 (1993)
Abstract
Related Products
ALX-380-055
Chromomycin A
3
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
•
MDR Other Products
ALX-620-050
Revised 02-Sep-05
Bisbenzimide H33342 . trihydrochloride
SYNONYMS
HOE 33342
2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-
bis
-1
H
-benzimidazole . 3HCl
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-620-050-M010
10 mg
22.00 USD
ALX-620-050-M050
50 mg
35.00 USD
Product Specification
FORMULA:
C
27
H
28
N
6
O . 3HCl
MW:
452.6 . 109.4
CAS NUMBER:
23491-52-3
PURITY:
≥95%
APPEARANCE:
Yellow to green powder.
SOLUBILITY:
Soluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light.
HAZARD:
TOXIC. MAY BE CARCINOGENIC.
Product Description
Cell permeable fluorescent dye that binds to A:T pairs in DNA [1]. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells [2]. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA [3]. Induces cell differentiation. About 10 times more cell permeable than bisbenzimide H33258 (Prod. No.
ALX-620-051
). Ex(max): 346nm; Em(max): 460nm.
Product Specific Literature References
[1]
Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis
:
S.A. Latt & G. Stetten; J. Histochem. Cytochem.
24
, 24 (1976)
Abstract
[2]
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells:
F. Belloc, et al.; Cytometry
17
, 59 (1994)
Abstract
[3]
Controlling gene expression in living cells through small molecule-RNA interactions
:
G. Werstuck & M.R. Green; Science
282
, 296 (1998)
Abstract
Further Categories Containing This Product:
Apoptosis Inducers & Inhibitors Other Products
•
MDR Other Products
ALX-610-045
Revised 16-Feb-05
B-Phycoerythrin
SYNONYMS
B-PE
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-045-M002
2 mg
35.00 USD
Product Specification
MW:
240kDa
SOURCE/HOST:
Isolated from unicellular red alga axenically grown under B-PE promoting conditions.
FORMULATION:
Liquid. 2mg in 2ml buffer.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light. Do not freeze.
Product Description
Stable and highly fluorescent label with large Stokes shift for conjugation to antibodies, other proteins and living cells. Ideal for use with argon or krypton lasers. Ex(max): 490-560nm; Em(max): 575nm.
ALX-610-025
Revised 03-Feb-05
Calcein
SYNONYMS
3,3'-
bis
[N,N-
bis
(Carboxymethyl)aminomethyl]fluorescein
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-025-G001
1 g
38.00 USD
Product Specification
FORMULA:
C
30
H
26
N
2
O
13
MW:
622.5
CAS NUMBER:
1461-15-0
PURITY:
≥95%
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
Product Description
For fluorometric determination of calcium and EDTA titration in presence of magnesium.
Product Specific Literature References
Indicator for titration of calcium in presence of magnesium with disodium dihydrogen ethylene diaminetetraacetate:
H. Diehl & J.L. Ellingboe; Anal. Chem.
28
, 882 (1956)
D.F.H. Wallach, et al.; Anal. Chem.
31
, 456 (1959)
Further Categories Containing This Product:
Calcium Indicators / Probes
•
MDR-associated Proteins [MRPs] / Related Products
•
P-glycoprotein [Pgp] / Related Products
ALX-610-026
Revised 03-Oct-08
Calcein AM
