ALEXIS Biochemicals: LY-83,583

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ALX-350-265

Revised 17-Dec-07

Phalloidin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Cytoskeletal Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-265-M001		1 mg	135.00 USD

Product Specification

FORMULA:	C₃₅H₄₈N₈O₁₁S
MW:	788.9
CAS NUMBER:	17466-45-4
MERCK INDEX:	14: 7196
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥95%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol, 100% ethanol or water (warm).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	VERY TOXIC.

Product Description

Binds to polymeric actin with high affinity. Stabilizes filaments and decreases concentration of monomeric actin.

Product Specific Literature References

Interaction of phalloidin with actin: A.M. Lengsfeld, et al.; PNAS 71, 2803 (1974) Abstract
Interaction of actin with phalloidin: polymerization and stabilization of F-actin: P. Dancker, et al.; Biochim. Biophys. Acta 400, 407 (1975) Abstract
Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth: J. Wehland, et al.; PNAS 74, 5613 (1977) Abstract
What we have learned from phalloidin: M. Frimmer; Toxicol Lett. 35, 169 (1987) Abstract
Effects of cytochalasin and phalloidin on actin: J.A. Cooper; J. Cell. Biol. 105, 1473 (1987), (Review) Abstract
Position and orientation of phalloidin in F-actin determined by X-ray fiber diffraction analysis: T. Oda, et al.; Biophys. J. 88, 2727 (2005) Abstract

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • Actin/Related Products

ALX-350-266

Revised 01-Jun-06

Phalloidin, Amino-, . hydrochloride
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Cytoskeletal Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-266-M001		1 mg	520.00 USD

Product Specification

FORMULA:	C₃₅H₄₉N₉O₁₀S . HCl
MW:	787.9 . 36.5
SOURCE/HOST:	Semisynthetic from phalloidin.
PURITY:	≥90% (HPLC)
APPEARANCE:	Amorphous solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from moisture.
HAZARD:	VERY TOXIC.

Product Description

Biological activity like phalloidin (Prod. No. ALX-350-265), but contains a functional amino group for coupling reactions.

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • Actin/Related Products

ALX-350-267

Revised 08-Apr-08

Phalloidin (Biotin)
SYNONYMS	Biotinyl-hexamethylenamidosuccinyl-aminomethyldithiolano-phalloidin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Cytoskeletal Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-267-MC01		0.1 mg	175.00 USD

Product Specification

FORMULA:	C₅₇H₈₃N₁₃O₁₃S₄
MW:	1286.6
SOURCE/HOST:	Semisynthetic from aminomethyldithiolano-phalloidin.
PURITY:	≥90%
APPEARANCE:	Amorphous solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Prepare fresh solutions for each application.
HANDLING:	Protect from light and moisture.
HAZARD:	VERY TOXIC.

Product Description

Binds actin filaments and streptavidin simultaneously, due to the long spacer between the two active components.

Product Specific Literature References

Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis: H. Faulstich, et al.; Exp. Cell Res. 144, 73 (1983) Abstract
Biotinylphallotoxins: preparation and use as actin probes: H. Faulstich, et al.; J. Histochem. Cytochem. 37, 1035 (1989) Abstract

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • Biotinylated Reagents Other Products • Actin/Related Products

ALX-350-268

Revised 01-Jun-06

Phalloidin (FITC)
SYNONYMS	Fluoresceinyl-aminomethyldithiolano-phalloidin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Cytoskeletal Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-268-MC01		0.1 mg	150.00 USD

Product Specification

FORMULA:	C₅₈H₆₂N₁₀O₁₄S₄
MW:	1251.4
SOURCE/HOST:	Semisynthetic from aminomethyldithiolano-phalloidin.
PURITY:	≥90%
APPEARANCE:	Amorphous solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water (warm).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Prepare fresh solutions for each application.
HANDLING:	Protect from light and moisture.
HAZARD:	VERY TOXIC.

Product Description

For detection of microfilaments in the light microscope. Mixture of 4 isomers with lower affinity to actin than phalloidin (Prod. No. ALX-350-265).

Product Specific Literature References

Fluorescent phallotoxin, a tool for the visualization of cellular actin: E. Wulf, et al.; PNAS 76, 4498 (1979) Abstract
Fluorescent phallotoxins as probes for filamentous actin: H. Faulstich, et al.; J. Muscle Res. Cell Motil. 9, 370 (1988) Abstract

Further Categories Containing This Product:

Dyes/Stains/Fluorescent Probes/Fluorescent Labels • Amanitins & Phalloidins/Related Products • Actin/Related Products

ALX-350-269

Revised 16-Aug-07

Phallacidin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Cytoskeletal Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-269-M001		1 mg	130.00 USD

Product Specification

FORMULA:	C₃₇H₅₀N₈O₁₃S
MW:	846.9
CAS NUMBER:	26645-35-2
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HAZARD:	VERY TOXIC.

Product Description

Biological activity like phalloidin (Prod. No. ALX-350-265), but contains a carboxy group for coupling reactions.

Product Specific Literature References

Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin: L.S. Barak, et al.; PNAS 77, 980 (1980) Abstract
Phallacidin prevents thrombin-induced increases in endothelial permeability to albumin: P.G. Phillips, et al.; Am. J. Physiol. 257, C562 (1989) Abstract

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • Actin/Related Products

ALX-350-270

Revised 08-Feb-08

α-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation/Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-270-M001		1 mg	140.00 USD

Product Specification

FORMULA:	C₃₉H₅₄N₁₀O₁₄S
MW:	919.0
CAS NUMBER:	23109-05-9
MERCK INDEX:	14: 373
RTECS:	BD6195000
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
APPEARANCE:	White to light yellow powder.
SOLUBILITY:	Soluble in 100% ethanol (5mg/ml), DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Inhibits eukaryotic RNA-polymerase II with high efficiency, but not eukaryotic RNA-polymerase I, or bacterial or viral RNA-polymerases. Inhibits mammalian protein synthesis.

Product Specific Literature References

Effect of alpha-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver: F. Stirpe & L. Fiume; Biochem. J. 103, 67P (1967) Abstract
Specific inhibition of nuclear RNA polymerase II by alpha-amanitin: T.J. Lindell, et al.; Science 170, 447 (1970) Abstract
Fifty years of amanitin: T. Wieland & H. Faulstich; Experientia 47, 1189 (1991), (Review) Abstract
Alpha-amanitin blocks translocation by human RNA polymerase II: X.Q. Gong, et al.; J. Biol. Chem. 279, 27422 (2004) Abstract

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • RNA Polymerases/Related Products • Protein Synthesis Other Products

ALX-350-271

Revised 08-Oct-07

β-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation/Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-271-M001		1 mg	170.00 USD

Product Specification

FORMULA:	C₃₉H₅₃N₉O₁₅S
MW:	920.0
CAS NUMBER:	21150-22-1
MERCK INDEX:	14: 373
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
FORMULATION:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Biological activity like α-amanitin (Prod. No. ALX-350-270), but contains a carboxy group which is useful for coupling reactions.

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • RNA Polymerases/Related Products • Protein Synthesis Other Products

ALX-350-272

Revised 17-Dec-07

γ-Amanitin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation/Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-272-M001		1 mg	170.00 USD

Product Specification

FORMULA:	C₃₉H₅₄N₁₀O₁₃S
MW:	903.0
CAS NUMBER:	13567-11-8; 21150-23-2
MERCK INDEX:	14: 373
SOURCE/HOST:	Isolated from Amanita phalloides mushrooms.
PURITY:	≥90%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dilute with buffer immediately prior to use. Product is destroyed by concentrated acid or base.
HANDLING:	Protect from light.
HAZARD:	VERY TOXIC.

Product Description

Biological activity like α-amanitin (Prod. No. ALX-350-270).

Further Categories Containing This Product:

Amanitins & Phalloidins/Related Products • RNA Polymerases/Related Products • Protein Synthesis Other Products

ALX-350-003

Revised 23-May-08

Okadaic acid (high purity)
SYNONYMS	Halochondrine A (high purity) 9,10-Deepithio-9,10-didehydroacanthifolicin (high purity)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Okadaic Acids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-003-C025		25 µg	60.00 USD
ALX-350-003-C050		50 µg	110.00 USD
ALX-350-003-C100		100 µg	180.00 USD
ALX-350-003-M001		1 mg	990.00 USD

Product Specification

FORMULA:	C₄₄H₆₈O₁₃
MW:	805.0
CAS NUMBER:	78111-17-8
MERCK INDEX:	14: 6819
RTECS:	AA8227800
SOURCE/HOST:	Isolated from Prorocentrum concavum by preparative flash, medium pressure and high performance liquid chromatography.
PURITY:	≥98% (HPLC)
APPEARANCE:	White crystalline solid.
SOLUBILITY:	Soluble in DMSO, 100% ethanol or methanol (1mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Use only fresh solutions.
HANDLING:	Packaged under inert gas. Protect from light.
HAZARD:	HIGHLY IRRITANT. MAY BE CARCINOGENIC. TOXIC.

Product Description

Potent inhibitor of protein phosphatases 1 (PP1) and 2A (PP2A) in numerous cell types. Does not affect activity of acid phosphatase, alkaline phosphatase and tyrosine phosphatase. Non-phorbol type tumor promoter. Induces apoptosis in human breast carcinoma cells (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T cell hybridomas. Tumor promoter. Has shown contractile effect on smooth muscle and heart muscle. Significantly increases cyclin B1 expression in adult neurons.

Product Specific Literature References

Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) Abstract
Protein phosphatases come of age: P. Cohen & P.T.W. Cohen; J. Biol. Chem. 264, 21435 (1989) Abstract; Full Text
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem. 58, 453 (1989) Abstract
Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism: T.A. Haystead, et al.; Nature 337, 78 (1989) Abstract
Okadaic acid: a new probe for the study of cellular regulation: P. Cohen, et al.; TIPS 15, 98 (1990), (Review) Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna, et al.; BBRC 189, 950 (1992) Abstract
Protein phosphatase inhibitors okadaic acid and calyculin A alter cell shape and F-actin distribution and inhibit stimulus-dependent increases in cytoskeletal actin of human neutrophils: P. Kreienbuehl, et al.; Blood 80, 2911 (1992) Abstract
Site-specific dephosphorylation of smooth muscle myosin light chain kinase by protein phosphatases 1 and 2A: M. Nomura, et al.; Biochemistry 31, 11915 (1992) Abstract
Inhibition of apoptosis in human tumour cells by okadaic acid: Q. Song, et al.; J. Cell Physiol. 153, 550 (1992) Abstract
Okadaic acid enhances human T cell activation and phosphorylation of an internal substrate induced by phorbol myristate acetate: Y. Tada, et al.; Immunopharmacol. 24, 17 (1992) Abstract
Okadaic acid inhibits glucocorticoid-induced apoptosis in T cell hybridomas at its late stage: Y. Ohoka, et al.; BBRC 197, 916 (1993) Abstract
Multiple apoptotic death types triggered through activation of separate pathways by cAMP and inhibitors of protein phosphatases in one (IPC leukemia) cell line: B.T. Gjertsen, et al.; J. Cell. Sci. 107, 3363 (1994) Abstract
Differential induction of apoptosis in human breast tumor cells by okadaic acid and related inhibitors of protein phosphatases 1 and 2A: K. Kiguchi, et al.; Cell Growth Differentiation 5, 995 (1994) Abstract
Unique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999), Review Abstract
Okadaic acid: the archetypal serine/threonine protein phosphatase inhibitor: A.B. Dounay & C.J. Forsyth; Curr. Med. Chem. 9, 1939 (2002), Review Abstract
Mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase: R. Tamaskovic, et al.; J. Biol. Chem. 278, 6710 (2003) Abstract; Full Text
Okadaic acid induced cyclin B1 expression and mitotic catastrophe in rat cortex: B. Chen, et al.; Neurosci. Lett. 406, 178 (2006) Abstract