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Biotinylated Reagents Other Products
You are here: Product Lines > Immunology > Immunological Methods > Biotin-(Strept)avidin Systems/Related Products > Biotinylated Reagents > Biotinylated Reagents Other Products
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ALX-350-267 Revised 08-Apr-08
Phalloidin (Biotin)
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SYNONYMS Biotinyl-hexamethylenamidosuccinyl-aminomethyldithiolano-phalloidin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products for Cytoskeletal Research
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ALX-350-267-MC01   0.1 mg 175.00 USD Add To Cart
Product Specification
FORMULA: C57H83N13O13S4
MW: 1286.6
SOURCE/HOST: Semisynthetic from aminomethyldithiolano-phalloidin.
PURITY: ≥90%
APPEARANCE: Amorphous solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, methanol or water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Prepare fresh solutions for each application.
HANDLING: Protect from light and moisture.
HAZARD: VERY TOXIC.

Product Description
Binds actin filaments and streptavidin simultaneously, due to the long spacer between the two active components.
Product Specific Literature References
Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis: H. Faulstich, et al.; Exp. Cell Res. 144, 73 (1983) Abstract
Biotinylphallotoxins: preparation and use as actin probes: H. Faulstich, et al.; J. Histochem. Cytochem. 37, 1035 (1989) Abstract
Further Categories Containing This Product:
Amanitins & Phalloidins/Related Products • Biotinylated Reagents Other Products • Actin/Related Products
 
 
ALX-610-035 Revised 03-Feb-05
DY-630-biotin
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-610-035-C500   500 µg 140.00 USD Add To Cart
Product Specification
FORMULA: C52H74N6O9S2
MW: 991.3
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Fluorescent label. Ex(max): ~630nm (red spectral region).
General Information
MANUFACTURER Manufactured by Dyomics GmbH.
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-610-042 Revised 03-Feb-05
DY-550-biotin
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PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes/Stains/Fluorescent Probes/Fluorescent Labels
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ALX-610-042-C500   500 µg 140.00 USD Add To Cart
Product Specification
FORMULA: C48H70N7O12S2Na
MW: 1024.2
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Fluorescent label. Ex(max): ~550nm.
General Information
MANUFACTURER Manufactured by Dyomics GmbH.
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-209-001 Revised 25-May-07
Calmodulin (bovine brain) (high purity) (Biotin)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Calmodulins
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ALX-209-001-C050   50 µg 180.00 USD Add To Cart
Product Specification
SOURCE/HOST: Isolated from bovine brain.
CONCENTRATION: 0.5mg/ml.
FORMULATION: Liquid. In PBS containing 0.1% BSA and 0.02% sodium azide.
APPLICATION: Detection of calmodulin-binding proteins by Western blot with streptavidin-alkaline phosphatase conjugates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase: R.L. Kincaid, et al.; Meth. Enzymol. 159, 605 (1988) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P02593: Calmodulin (human)
AfCS Signalling Gateway link A000452: Calmodulin I (mouse)
Further Categories Containing This Product:
Biotinylated Reagents Other Products
 
 
ALX-209-002 Revised 02-Aug-06
Annexin V (human) (recombinant) (Biotin)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V/Related Products
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ALX-209-002-T020   20 tests 106.00 USD Add To Cart
ALX-209-002-T100   100 tests 352.00 USD Add To Cart
ALX-209-002-T300   300 tests 531.00 USD Add To Cart
Product Specification
MW: ~35.8kDa.
QUANTITY: 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl.
PURITY: ≥98% (SDS-PAGE, HPLC)
FORMULATION: Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
SPECIFICITY: Binds to phosphatidylserine (PS).
APPLICATION: Detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem. 265, 4923 (1990) Abstract; Full Text
[2] Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol. 148, 2207 (1992) Abstract
[3] Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood 84, 1415 (1994) Abstract
[4] Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood 85, 532 (1995) Abstract
[5] A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth. 184, 39 (1995) Abstract
[6] Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med. 182, 1545 (1995) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[7] Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry 24, 123 (1996) Abstract
[8] CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol. 156, 4631 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered
through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281 : Annexin V (mouse)
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • Biotinylated Reagents Other Products
 
 
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