ALEXIS Biochemicals: Nerve Growth Factor 2.5S (mouse) Grade II

ALX-521-007

Revised 13-Mar-08

Nerve Growth Factor 2.5S (mouse) Grade II
SYNONYMS	NGF 2.5S (mouse) Grade II
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Nerve Growth Factors [NGF] & Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-521-007-C025		25 µg	180.00 USD
ALX-521-007-C100		100 µg	420.00 USD

Product Specification

MW:	~26kDa; homodimer.
CAS NUMBER:	93828-24-6
SOURCE/HOST:	Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).
QUANTITY:	Concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use.
BIOLOGICAL ACTIVITY:	Neurotrophic activity was bioassayed using rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium [1].
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.
HANDLING:	Avoid freeze/thaw cycles. Do not reuse medium containing mNGF. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

[1] Nerve growth factor: R.A. Bradshaw; Annu. Rev. Biochem. 47, 191 (1978) Abstract
[2] The changing scene of neurotrophic factors: H. Thoenen; TINS 14, 165 (1991), (Review) Abstract
[3] Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C. R. Acad. Sci. III 316, 1158 (1993), (Review) Abstract
[4] The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev. 18, 143 (1994), (Review) Abstract
[5] Neurotrophic factors enter the clinic: M. Barinaga; Science 264, 772 (1994), (Review) Abstract
[6] Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature 368, 193 (1994), (Review) Abstract
[7] Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J. 8, 738 (1994), (Review) Abstract
[8] Neurotrophic factors: two are better than one: R. Nishi; Science 265, 1052 (1994), (Review) Abstract

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