ALEXIS Biochemicals: Nerve Growth Factor 7S (mouse)

ALX-521-008

Revised 17-Oct-06

Nerve Growth Factor 7S (mouse)
SYNONYMS	NGF 7S (mouse)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Nerve Growth Factors [NGF] & Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-521-008-C100		100 µg	245.00 USD

Product Specification

MW:	~130kDa.
SOURCE/HOST:	Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).
QUANTITY:	Determined by Lowry and Pierce method.
PURITY:	≥97% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Dilute in sterile water or DMEM to a concentration of at least 10µg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation.
BIOLOGICAL ACTIVITY:	Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium (See [2]).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

[1] O.H. Lowry, et al.; J. Biol. Chem. 193, 265 (1951)
[2] The isolation of the mouse nerve growth factor protein in a high molecular weight form: S. Varon, et al.; Biochemistry 6, 2202 (1967) Abstract
[3] The changing scene of neurotrophic factors: H. Thoenen; TINS 14, 165 (1991), (Review) Abstract
[4] Towards a comprehensive understanding of the trophic support of motoneurons: H. Thoenen, et al.; C. R. Acad. Sci. III 316, 1158 (1993), (Review) Abstract
[5] Neurotrophic factors enter the clinic: M. Barinaga; Science 264, 772 (1994), (Review) Abstract
[6] Neurobiology. Tracking neurotrophin function: A.M. Davies; Nature 368, 193 (1994), (Review) Abstract
[7] Role of neurotrophins in mouse neuronal development: R. Klein; FASEB J. 8, 738 (1994), (Review) Abstract
[8] The neurotrophins and their receptors: structure, function, and neuropathology: L.M. Maness, et al.; Neurosci. & Biobehav. Rev. 18, 143 (1994), (Review) Abstract
[9] Neurotrophic factors: two are better than one: R. Nishi; Science 265, 1052 (1994), (Review) Abstract

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