ALEXIS Biochemicals: Nesfatin-1 (mouse/rat) Detection Set [For ELISA Application]

APO-54N-036

Revised 19-Jun-08

Nesfatin-1 (mouse/rat) Detection Set [For ELISA Application]
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Food Intake Modulators Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-036-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse Rat
SENSITIVITY:	0.3ng/ml (range 0 to 16ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of mouse or rat nesfatin-1 from biological samples.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

Brain hypothalamus expressed several secreted molecules that function in regulating feeding behavior [1]. NUCB2 (NUCB2 also called NEFA for DNA binding /EF-hand /acidic protein) is a hypothalamus-secreted protein containing 396 amino acids that is highly conserved in human, mice and rat [2, 3]. NUCB2 when injected directly into the brain of rats promotes anorexia and decreases body weight [3]. NUCB2 is cleaved posttranslationally by prohormone convertases into a N-terminus-fragment Nesfatin-1 and two C-terminal peptides, Nesfatin-2 and Nesfatin-3 [3]. Nesfatin-1 possesses all of the anorexigenic property of NUCB2 [4]. The conversion of NUCB2 into Nesfatin-1 is indispensable for its activity in vivo [3]. Nesfatin-1 is found in discrete nuclei of the hypothalamus where it probably activates a leptin-independent melanocortin pathway [3]. Nesfatin-1 crosses the Blood Brain Barrier (BBB) in both the blood-to-brain and brain-to-blood directions by a nonsaturable system [5]. NUCB2 is also expressed in the adipocyte cell line 3T3L1 suggesting other functions of Nesfatin-1 outside brain or peripheral source of Nesfatin-1 affecting brain function [6]. Nesfatin-1 in rat stimulates calcium influx and interacts with a G protein-coupled receptor still to be characterized [7].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor], Q9JI85: Nucleobindin-2 (rat) [Precursor]
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Hormonal regulation of food intake: S. Stanley, et al.; Physiol. Rev. 85, 1131 (2005) Abstract; Full Text
[2] Isolation, characterization, and primary structure of a calcium-binding 63-kDa bone protein: M. Wendel, et al.; JBC 270, 6125 (1995) Abstract; Full Text
[3] Identification of nesfatin-1 as a satiety molecule in the hypothalamus: S. Oh-I, et al.; Nature 443, 709 (2006) Abstract
[4] Serotonin systems upregulate the expression of hypothalamic NUCB2 via 5-HT2C receptors and induce anorexia via a leptin-independent pathway in mice: K. Nonogaki, et al.; BBRC 372, 186 (2008) Abstract
[5] Permeability of the blood-brain barrier to a novel satiety molecule nesfatin-1: T.O. Price, et al.; Peptides 28, 2372 (2007) Abstract
[6] In-depth analysis of the adipocyte proteome by mass spectrometry and bioinformatics: J. Adachi, et al.; Mol. Cell. Proteomics 6, 1257 (2007) Abstract; Full Text
[7] Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain: G.C. Brailoiu, et al.; Endocrinology 148, 5088 (2007) Abstract

ALX-522-114	Nesfatin-1 (mouse) (recombinant) (tagged)
ALX-522-112	Nesfatin-1 (mouse) (recombinant)
ALX-210-944	Polyclonal Antibody to Nesfatin-1 (AT120)
ALX-804-854	Monoclonal Antibody to Nesfatin-1 (Nesfaty-1)
ALX-522-118	Nesfatin-1 (rat) (recombinant) (tagged)
ALX-522-116	Nesfatin-1 (rat) (recombinant)