ALEXIS Biochemicals: Netrin-1 (chicken) (recombinant)

ALX-522-106

Revised 19-Jun-08

Netrin-1 (chicken) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Netrins & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-106-2010		2x10 µg	350.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
MW:	~80kDa
SOURCE/HOST:	Produced in HEK 293 cells. Chicken netrin-1 (aa 27-606) is fused at the C-terminus to a linker peptide (6 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
RECONSTITUTION:	Reconstitute with 100µl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
SPECIFICITY:	Binds to human receptor UNC5B.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

General Information

The Netrins (whose name means “one who guides” in Sanskrit) are a family of laminin-related secreted protein. In mice, human and rat, netrin-1, netrin-3/NTL2, netrin-4 and G-netrins have been cloned. Netrin-1 is predominantly expressed by floor plate cells as commissural axons extend toward the ventral midline. Netrin-1 induces axonal outgrowth, axon orientation and neuronal migration in neuronal development. It is chemoattractive for some neurons and chemorepellent for others. To carry its functions, netrin-1 interacts with specific receptors: DCC/neogenin and UNC-5. These receptors belong to the family of “dependence receptors” that induce apoptosis in the absence of their respective ligand, and promote survival when bound to their ligand. The Netrin-1 mediated survival signal inhibits p53-induced apoptosis. Netrin-1 is also expressed in organs such as pancreas, lung, bowel, bone and mammary gland. In these tissues, netrin-1 has different roles such as invasion of epithelial cells, leukocytes migration, cell adhesion or angiogenesis. Netrin-1, as a survival factor, also contributes to the control of tumorigenesis. Chicken netrin-1 shares 87% aa identity with mouse and human netrin-1.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q90922: Netrin-1 (chicken) FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Netrin-1 and its receptors in tumorigenesis: H. Arakawa; Nat. Rev. Cancer 4, 978 (2004), (Review) Abstract
Netrin-1: when a neuronal guidance cue turns out to be a regulator of tumorigenesis: P. Mehlen & C. Furne; Cell. Mol. Life Sci. 62, 2599 (2005), (Review) Abstract

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