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ALX-804-177 Revised 28-Aug-08
Monoclonal Antibody to VASP (IE273)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein MAb (IE273)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-177-C050   50 µg 295.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Pig
Others
CLONE: IE273
ISOTYPE: Mouse IgG1
CONCENTRATION: 500µg/ml
FORMULATION: Liquid. In PBS containing 0.01% sodium azide.
IMMUNOGEN: Purified human VASP (vasodilator stimulated phosphoprotein).
SPECIFICITY: Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human, rabbit, pig and bovine VASP.
APPLICATION: Immunocytochemistry (1-5μg/ml)
Immunoprecipitation
Western Blot (0.1-0.25μg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).
Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
Product Specific Literature References
Monoclonal antibodies against the focal adhesion protein VASP revealing epitopes involved in the interaction with two VASP binding proteins and VASP phosphorylation: Abel, et al.; Eur. J. Cell Biol. 69 (Suppl. 42), 39a (1996) Abstract
A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family: K. Niebuhr, et al.; EMBO J. 16, 5433 (1997) Abstract
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Sitmulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001) Abstract; Full Text
General Information
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif,
b) a more divergent proline-rich central domain (which is responsible for profilin binding), and
c) a conserved C-terminal EVH2 domain.

Particularly high VASP levels are present in platelets, although VASP is expressed in a wide variety of cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.

Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-403 Revised 11-Dec-07
Monoclonal Antibody to VASP (phosphorylated) (pSer157) (5C6)
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SYNONYMS anti-Vasodilator-stimulated Phosphoprotein (phosphorylated) (pSer157) MAb (5C6)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-403-C100   100 µg 506.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 5C6
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of human VASP (vasodilator-stimulated phosphoprotein) phosphorylated at Ser157.
SPECIFICITY: Recognizes human and mouse Ser157-phosphorylated VASP. Does not cross-react with the non-phosphorylated form of VASP.
APPLICATION: Flow Cytometry
Immunocytochemistry (1-10µg/ml; may tolerate 0.5% formaldehyde fixation).
Immunoprecipitation
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection).
Functional Application: Monitoring of PKA activity (see [1,2]).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
[1] Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I: A. Smolenski, et al.; J. Biol. Chem. 275, 25723 (2000) Abstract; Full Text
[2] KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION The use of MAb to VASP (activated) (Ser157) (5C6) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-240 Revised 05-Aug-08
Monoclonal Antibody to VASP (phosphorylated) (pSer239) (16C2)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein (phosphorylated) (pSer239) MAb (16C2)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-240-C100 Purified 100 µg 506.00 USD Add To Cart
ALX-804-240B-C100 Biotin 100 µg 580.00 USD Add To Cart
ALX-804-240F-C100 FITC 100 µg 580.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: 16C2
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: 16C2: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose. Reconstitute with 1ml distilled water (15 min, RT).
16C2-Biotin and -FITC: Liquid. 0.5mg/ml in 2x PBS containing 0.09% sodium azide, PEG and sucrose.
IMMUNOGEN: Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE).
SPECIFICITY: Recognizes human, mouse, rat and rabbit Ser239-phosphorylated VASP. Does not cross-react with the non-phosphorylated form of VASP. 
APPLICATION: Flow Cytometry
Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation) 
Immunoprecipitation
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20.
Functional Application: Use to monitor PKG/PKA activity.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem. 273, 20029 (1998) Abstract; Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost. 82, 1145 (1999) Abstract
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000) Abstract; Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001) Abstract; Full Text
Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem. 276, 15666 (2001) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-210-880 Revised 20-May-08
Polyclonal Antibody to VASP (affinity purified)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein (affinity purified)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-880-C025   25 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
SOURCE/HOST: From rabbit.
CONCENTRATION: 250µg/ml.
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN: Recombinant human VASP.
SPECIFICITY: Recognizes human, mouse and pig VASP. Detects both the 46kDa (Ser157 dephospho) and 50kDa (Ser157 phospho) forms of VASP.
APPLICATION: Immunocytochemistry (formaldehyde fixed cells; 1µg/ml)
Immunoprecipitation (2µg/ml)
Western Blot (0.1µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
POSITIVE CONTROL: Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).
Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
General Information
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-898 Revised 11-Sep-07
Polyclonal Antibody to VASP (human)
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SYNONYMS anti-Vasodilator Stimulated Phosphoprotein (human) PAb
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-898-R100   100 µl 225.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.02% sodium azide.
IMMUNOGEN: Recombinant human VASP (vasodilator stimulated phosphoprotein) fused to a His-tag.
SPECIFICITY: Recognizes unphosphorylated as well as phosphorylated (Ser157) human VASP. Does not cross-react with mouse or rat VASP. For the detection of mouse VASP use Prod. No. ALX-210-880.
APPLICATION: Immunocytochemistry (1:500-1:‘000)
Western Blot (1:1’500-1:3’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt).
Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
Product Images
Please click on thumbnails to enlarge.
General Information
VASP (vasodilator stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. Phosphorylation of VASP at Ser-157 causes a mobility shift in SDS gel electrophoresis from 46 to 50 kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity. VASP is the founding member of the Ena-VASP protein family, comprising the Drosophila protein Enabled (Ena), its mouse homologue Mena (mammalian Enabled), and mouse EVL (Ena-VASP-like protein). With these proteins VASP shares a conserved overall domain organization:
a) the conserved N-terminal Ena-VASP homology domain 1 (EVH1), which mediates binding to a novel proline-rich motif
b) a more divergent proline-rich central domain (which is responsible for profilin binding)
c) a conserved C-terminal EVH2 domain.

VASP is expressed in a variety of mammalian cell types and tissues. In cultured cells, VASP is associated with focal adhesions, cell-cell contacts, microfilaments, and highly dynamic membrane regions. From in vitro binding data VASP has been suggested to link profilin to zyxin, vinculin, and the Listeria spp. surface protein ActA, respectively. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation and the actin-dependent motility of intracellular bacterial pathogens. 
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-725 Revised 25-Feb-08
Polyclonal Antibody to VASP (M4) - Replaced by Prod. No. ALX-210-898
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
not sold in USA
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-840-008 Revised 03-Mar-08
Pre-immune Serum to PAb to VASP
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SYNONYMS Pre-immune Serum to PAb to Vasodilator Stimulated Phosphoprotein
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY VASP/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-008-R025   25 µl 65.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
FORMULATION: Liquid, sterile filtered. Contains 0.01% sodium azide.
APPLICATION: Negative control for polyclonal antibody to VASP (Prod. No. ALX-210-898).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Related Products
 
 
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