ALEXIS Biochemicals: Nitroso-thiol (RSNOs) Detection Kit

ALX-850-037

Revised 26-Jun-08

Nitroso-thiol (RSNOs) Detection Kit
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Oxidative Stress Markers & Reagents/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-037-KI01		1 Kit	590.00 USD

Product Specification

QUANTITY:	96 wells (~80 tests).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light. Store kit at +4°C. Store standard (vial 5) at -20°C

Product Description

The assay of nitroso-thiols in many biological fluids, appears to provide a very useful index of NOS activity. The concentration of RSNOs has been shown to increase with localized induction of NOS and to fall rapidly upon administration of NOS inhibitors.
The kit provides a complete solution for the assay of high or low MW RSNOs. The assay is divided essentially into two steps. In the first step, the sample is prepared according to which subgroup of RSNO is desired. The S-N bond is then cleaved, resulting in the formation of an equivalent of nitrite, which is assayed using the Griess reaction. The intensity of the color formed is linearly related to the concentration of nitrite, and hence, to the original RSNO concentration.

Product Specific Literature References

Increased nitrosothiols in exhaled breath condensate in inflammatory airway diseases: M. Corradi, et al.; Am. J. Respir. Crit. Care Med. 163, 854 (2001) Abstract; Full Text
Dose-dependent onset and cessation of action of inhaled budesonide on exhaled nitric oxide and symptoms in mild asthma: S.A. Kharitonov, et al.; Thorax 57, 889 (2002) Abstract

General Information

MANUFACTURER

Manufactured by Oxonon.

General Literature References

Detection of S-nitrosothiols by fluorometric and colorimetric methods: D.A. Wink, et al.; Methods Enzymol. 301, 201 (1999) Abstract
Fluorometric detection of S-nitrosothiols: P. Kostka and J.K. Park; Methods Enzymol. 301, 227 (1999) Abstract
Mechanisms of biological S-nitrosation and its measurement: T. Akaike; Free Radic. Res. 33, 461 (2000) Abstract

Further Categories Containing This Product:

Nitric Oxide & NOS Detection/Related Products • Quantification Kits

another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.