ALEXIS Biochemicals: ODN 2216 (TLRgrade™) (synthetic)

ALX-746-005

Revised 06-Oct-06

ODN 2216 (TLRgrade™) (synthetic)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Oligodeoxynucleotides [ODNs]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-746-005-T100		100 tests	155.00 USD

Product Specification

SEQUENCE:	5’-ggGGGACGATCGTCgggggg-3’ (lower case letters indicate phosphorothioate linkage).
MW:	6644 (ammonium salt)
SOURCE/HOST:	Synthetic.
QUANTITY:	14.9nmol (~99µg). Sufficient for at least 100 cellular activation assays at 0.1-0.5µg/well when performed in a 200µl assay volume (i.e. 96 well microtiter plate).
PURITY DETAIL:	Activity and endotoxin tested - TLRgrade™.
FORMULATION:	Lyophilized. Sterile.
ENDOTOXIN CONTENT:	<0.02 EU/µg (LAL test; BioWhittaker)
RECONSTITUTION:	For a 100µM stock solution, dissolve the total vial content in 149µl endotoxin-free water (included) or PBS (to order seperately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING:	Protect from light. After reconstitution, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Product Description

CpG oligodeoxynucleotide (Type A) with mixed phosphodiester/phosphorothioate backbone. Specific ligand for human TLR9 (Toll-like receptor 9). For inactive control compound see ODN 2243 (Prod. No. ALX-746-205).

Product Specific Literature References

CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro: S. Rothenfusser, et al.; Blood 103, 2162 (2004) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).

Related Products:

- PBS (endotoxin-free) (Prod. No. ALX-505-007)

- ODN 2243 (TLRgrade™) (synthetic) (Control) (Prod. No. ALX-746-205)

- iODN (ttaggg)₄ (TLRgrade™) (synthetic) (Inhibitory for human) (Prod. No. ALX-746-251)

ALX-505-008	ddWater (endotoxin-free)
ALX-505-007	PBS (endotoxin-free)
ALX-746-251	iODN (ttaggg)₄ (TLRgrade™) (synthetic) (Inhibitory for human)
ALX-746-205	ODN 2243 (TLRgrade™) (synthetic) (Control)