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ALX-804-276
Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (HGAC85)
SYNONYMS
anti-Glc-NAc (O-linked) MAb (HGAC85)
PRODUCT LINE
Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY
Protein Glycosylation
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-276-C100
100 µg
379.00 USD
Product Specification
CLONE:
HGAC85
ISOTYPE:
Mouse IgG3
CONCENTRATION:
2mg/ml
FORMULATION:
Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:
Heat killed, pepsin treated group A streptococci.
SPECIFICITY:
Recognizes β-1,3 linked O-GlcNAc (O-linked N-acetylglucosamine) residues of streptococcal group A carbohydrates as well as O-GlcNAc glycosylated proteins.
APPLICATION:
ELISA
Immunocytochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1µg/ml)
Note:
Do not use with diluents containing glycosylated proteins!
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier:
X. Zhang & V. Bennett; J. Biol. Chem.
271
, 31391 (1996)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-111
Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)
SYNONYMS
anti-Glc-NAc (O-linked) MAb (RL2)
PRODUCT LINE
Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY
Protein Glycosylation
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-111-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE:
RL2
ISOTYPE:
Mouse IgG1
CONCENTRATION:
2mg/ml
FORMULATION:
Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
SPECIFICITY:
Recognizes human, mouse and rat nuclear pore complex (NPC), cytoplasmic and intracellular O-GlcNAc glycosylated proteins. Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot.
APPLICATION:
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (1:200))
Immunoprecipitation
Western Blot (1:1’000)
Note:
Do not use with diluents containing glycosylated proteins.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins:
C.M. Snow, et al.; J. Cell. Biol.
104
, 1143 (1987)
Abstract
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1:
G. Majumdar, et al.; Diabetes
53
, 3184 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-144
Revised 07-Nov-05
Monoclonal Antibody to ACRP30headless (human) (Ne.Na)
SYNONYMS
anti-ACRP30
headless
(human) MAb (Ne.Na)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-144-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Ne.Na
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE).
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human ACRP30
headless
(tail adiponectin) (Prod. No.
ALX-203-002
).
SPECIFICITY:
Recognizes human ACRP30
headless
. Does not cross-react with mouse ACRP30
headless.
Does not cross-react with full length adiponectin.
APPLICATION:
ELISA (capture)
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-515
Revised 30-Aug-06
Monoclonal Antibody to Adiponectin (rat) (RADI 06)
SYNONYMS
anti-ACRP30 (rat) MAb (RADI 06)
anti-Adipocyte Complement Related Protein of 30kDa (rat) MAb (RADI 06)
anti-AdipoQ (rat) MAb (RADI 06)
anti-apM1 (rat) MAb (RADI 06)
anti-GBP28 (rat) MAb (RADI 06)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-515-C050
50 µg
195.00 USD
ALX-804-515-C100
100 µg
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE:
RADI 06
ISOTYPE:
Mouse IgG2
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant rat adiponectin.
SPECIFICITY:
Recognizes both natural and recombinant rat adiponectin.
APPLICATION:
ELISA (direct and indirect: 1:5’000-1:10’000).
Western Blot (1:2’000-10’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Western Blot Protocol:
1: SDS-PAGE Sample buffer
62.5mM Tris-HCl (pH 6.8), 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.1% (w/v) bromophenol blue.
2: Serum preparation
A. 1µl rat serum and 9µl sample buffer after boiling for 5 min.
B. Recombinant rat adiponectin protein: 100ng/µl and 9µl sample buffer after boiling for 5 min.
3: Electrophoresis
4: Transfer
A. Nitrocellulose membrane
B. Constant 30V for 16 hours.
5: Western blotting
A. Blocking: 0.5% skim milk in TBST
B. Secondary IgG: anti-mouse IgG whole molecular (1:5’000)
C. ECL reaction
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-453
Revised 15-Nov-07
Monoclonal Antibody to Adiponectin (human) (HADI 741)
SYNONYMS
anti-ACRP30 (human) MAb (HADI 741)
anti-Adipocyte Complement Related Protein of 30kDa (human) MAb (HADI 741)
anti-AdipoQ (human) MAb (HADI 741)
anti-apM1 (human) MAb (HADI 741)
anti-GBP28 (human) MAb (HADI 741)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-453-C050
50 µg
195.00 USD
ALX-804-453-C100
100 µg
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
HADI 741
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1
mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant human adiponectin.
SPECIFICITY:
Recognizes the C-terminal globular domain of both natural and recombinant human adiponectin. Cross-reactivity with other species not tested.
APPLICATION:
ELISA
(direct and indirect: 1:2’000-1:10’000)
Western Blot
(1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus
:
B.S. Youn, et al.; J. Clin. Endocrinol. Metab.
89
, 150 (2004)
Abstract
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-452
Revised 24-Oct-05
Monoclonal Antibody to Adiponectin (human) (HADI 773)
SYNONYMS
anti-ACRP30 (human) MAb (HADI 773)
anti-Adipocyte Complement Related Protein of 30kDa (human) MAb (HADI 773)
anti-AdipoQ (human) MAb (HADI 773)
anti-apM1 (human) MAb (HADI 773)
anti-GBP28 (human) MAb (HADI 773)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-452-C050
50 µg
195.00 USD
ALX-804-452-C100
100 µg
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
HADI 773
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant human adiponectin.
SPECIFICITY:
Recognizes both natural and recombinant human adiponectin. Cross-reactivity with other species not tested.
APPLICATION:
ELISA
(direct and indirect: 1:5'000-1:10'000).
Immunohistochemistry
(paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature).
Western Blot
(1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Plasma resistin concentrations measured by enzyme-linked immunosorbent assay using a newly developed monoclonal antibody are elevated in individuals with type 2 diabetes mellitus
:
B.S. Youn, et al.; J. Clin. Endocrinol. Metab.
89
, 150 (2004)
Abstract
Adiponectin expression in human fetal tissues during mid- and late gestation
:
S. Corbetta, et al.; J. Clin. Endocrinol. Metab.
90
, 2397 (2005)
Abstract
;
Full Text
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-513
Revised 13-Aug-08
Monoclonal Antibody to Adiponectin (mouse) (MADI 04)
SYNONYMS
anti-ACRP30 (mouse) MAb (MADI 04)
anti-Adipocyte Complement Related Protein of 30kDa (mouse) MAb (MADI 04)
anti-AdipoQ (mouse) MAb (MADI 04)
anti-apM1 (mouse) MAb (MADI 04)
anti-GBP28 (mouse) MAb (MADI 04)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-513-C050
50 µg
220.00 USD
ALX-804-513-C100
100 µg
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
MADI 04
ISOTYPE:
Rat IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant mouse adiponectin.
SPECIFICITY:
Recognizes both natural and recombinant mouse adiponectin. Weakly cross-reacts with human and rat adiponectin.
APPLICATION:
ELISA
(direct and indirect: 1:5’000-1:10’000)
Immunoprecipitation
(1-5μl per sample in a final reaction volume of 500-1’000μl)
Western Blot
(1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-454
Revised 14-Jul-08
Monoclonal Antibody to Adiponectin (mouse) (MADI 1147)
SYNONYMS
anti-ACRP30 (mouse) MAb (MADI 1147)
anti-Adipocyte Complement Related Protein of 30kDa (mouse) MAb (MADI 1147)
anti-AdipoQ (mouse) MAb (MADI 1147)
anti-apM1 (mouse) MAb (MADI 1147)
anti-GBP28 (mouse) MAb (MADI 1147)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-454-C050
50 µg
195.00 USD
ALX-804-454-C100
100 µg
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
MADI 1147
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant mouse adiponectin.
SPECIFICITY:
Recognizes both natural and recombinant mouse adiponectin. Detects a band of ~30kDa by Western blot. Weakly cross-reacts with human and rat adiponectin.
APPLICATION:
ELISA
(direct and indirect: 1:5'000-1:10'000).
Western Blot
(1:5'000-1:10'000 using ECL; suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-720
Revised 29-May-07
Monoclonal Antibody to Adiponectin (rat) (RADI 264)
SYNONYMS
anti-ACRP30 (rat) MAb (RADI 264)
anti-Adipocyte Complement Related Protein of 30kDa (rat) MAb (RADI 264)
anti-AdipoQ (rat) MAb (RADI 264)
anti-apM1 (rat) MAb (RADI 264)
anti-GBP28 (rat) MAb (RADI 264)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Adiponectin [ACRP30]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-720-C050
50 µg
195.00 USD
ALX-804-720-C100
100 µg
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE:
RADI 264
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant rat adiponectin.
SPECIFICITY:
Recognizes rat adiponectin. Does not cross-react with human or mouse adiponectin.
APPLICATION:
ELISA
(direct and indirect: 1:5’000-1:10’000)
Western Blot
(1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Related Products
ALX-201-303
Adiponectin (rat) (recombinant)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-635
Revised 18-Jan-07
Monoclonal Antibody to ANGPTL3 (human) (1D10)
SYNONYMS
anti-Angiopoietin-like Protein 3 (human) MAb (1D10)
anti-Angiopoietin-5 (human) MAb (1D10)
anti-ANG-5 (human) MAb (1D10)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Angiopoietin-like Proteins [ANGPTLs]/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-635-R050
50 µl
195.00 USD
