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Leptin / Related Products
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Items 17 of 17
ALX-163-003 Revised 06-Jun-03
NPY (human)
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SYNONYMS Neuropeptide Y (human)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Ca2+ Modulators
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ALX-163-003-PC05   0.5 mg* 140.00 USD Add To Cart
ALX-163-003-P001   1 mg* 250.00 USD Add To Cart
Product Specification
SEQUENCE: H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr-NH2
FORMULA: C189H285N55O57S
MW: 4271.7
CAS NUMBER: 90880-35-6
MERCK INDEX: 14: 6485
QUANTITY: *Sold as net peptide weight.
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Specific Literature References
Cloning, characterization, and DNA sequence of a human cDNA encoding neuropeptide tyrosine: C.D. Minth, et al.; PNAS 81, 4577 (1984) Abstract
Further Categories Containing This Product:
Leptin / Related Products • Peptides • Neuropeptides & Receptors Other Products
 
 
ALX-201-034 Revised 02-May-06
Leptin (human) (recombinant)
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SYNONYMS OB Gene Product (human) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-201-034-M001   1 mg 190.00 USD Add To Cart
Product Specification
MW: ~16kDa.
MERCK INDEX: 14: 5443
SOURCE/HOST: Produced in E. coli. Homologous to human serum leptin.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized from sterile filtered solution in 10mM sodium citrate, pH 4.0.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein.
RECONSTITUTION: Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing 0.1% HSA or BSA.
BIOLOGICAL ACTIVITY: Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted protein is stable for 4 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
General Information
Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P41159: Leptin (human) (precursor)
General Literature References
Leptin and the regulation of body weight in mammals: J.M. Friedman and J.L. Halaas; Nature 395, 763 (1998), review Abstract
Mutation screening and identification of a sequence variation in the human ob gene coding region: R.V. Considine, et al.; BBRC 220, 735 (1996) Abstract
Serum immunoreactive leptin concentrations in normal-weight and obese humans  : R.V. Considine, et al. ; New Engl. J. Med. 334, 292 (1996) Abstract
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects  : M. Maffei, et al.; Nature Medicine 1, 1155 (1995) Abstract
Effects of the obese gene product on body weight regulation in ob/ob mice  : M.A. Pelleymounter, et al. ; Science 269, 540 (1995) Abstract
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity  : R.V. Considine, et al. ; J. Clin. Invest 95, 2986 (1995) Abstract
Overexpression of the obese (ob) gene in adipose tissue of the human subjects  : F. Lönnqvist, et al. ; Nature Medicine 1, 950 (1995) Abstract
Positional cloning of the mouse obese gene and its human homologue  : Y. Zhang, et al.; Nature 372, 425 (1994) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-035 Revised 18-May-06
Leptin (mouse) (recombinant)
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SYNONYMS OB Gene Product (mouse) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-201-035-M001   1 mg 190.00 USD Add To Cart
Product Specification
MW: ~16kDa.
MERCK INDEX: 14: 5443
SOURCE/HOST: Produced in E. coli. Homologous to mouse serum leptin.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized from 0.0045mM sodium bicarbonate.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein.
RECONSTITUTION: Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing a carrier protein.
BIOLOGICAL ACTIVITY: Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted protein is stable for 4 weeks when stored at +4°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. Studies have shown that it may also influence reproductive function. Mice with ob/ob genotype develop a form of diabetes similar to type II in humans.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link 41160: Leptin (mouse) (precursor)
AfCS Signalling Gateway link A001397: Leptin (mouse)
General Literature References
Positional cloning of the mouse obese gene and its human homologue: Y. Zhang, et al.; Nature 372, 425 (1994) Abstract
Effects of the obese gene product on body weight regulation in ob/ob mice: M.A. Pelleymounter, et al.; Science 269, 540 (1995) Abstract
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects: M. Maffei, et al.; Nature Med. 1, 1155 (1995) Abstract
Weight-reducing effects of the plasma protein encoded by the obese gene: J.L. Halaas, et al.; Science 269, 543 (1995) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-231 Revised 18-Mar-08
Leptin (rat) (recombinant)
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SYNONYMS OB Gene Product (rat) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-201-231-M001   1 mg 190.00 USD Add To Cart
Product Specification
MW: ~16kDa.
MERCK INDEX: 14: 5443
SOURCE/HOST: Produced in E. coli. Homologous to rat serum leptin.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized from 0.0045mM sodium bicarbonate.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein.
RECONSTITUTION: Reconstitute with sterile water or 0.4% sodium bicarbonate, pH 8-9. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing a carrier protein.
BIOLOGICAL ACTIVITY: Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted protein is stable for 4 weeks when stored at +4°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P50596: Leptin (rat) (precursor)
General Literature References
Leptin and the regulation of body weight in mammals: J.M. Friedman and J.L. Halaas; Nature 395, 763 (1998), review Abstract
Mutation screening and identification of a sequence variation in the human ob gene coding region: R.V. Considine, et al.; BBRC 220, 735 (1996) Abstract
Serum immunoreactive leptin concentrations in normal-weight and obese humans  : R.V. Considine, et al. ; New Engl. J. Med. 334, 292 (1996) Abstract
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects  : M. Maffei, et al.; Nature Medicine 1, 1155 (1995) Abstract
Effects of the obese gene product on body weight regulation in ob/ob mice  : M.A. Pelleymounter, et al. ; Science 269, 540 (1995) Abstract
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity  : R.V. Considine, et al. ; J. Clin. Invest 95, 2986 (1995) Abstract
Overexpression of the obese (ob) gene in adipose tissue of the human subjects  : F. Lönnqvist, et al. ; Nature Medicine 1, 950 (1995) Abstract
Positional cloning of the mouse obese gene and its human homologue  : Y. Zhang, et al.; Nature 372, 425 (1994) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-804-520 Revised 31-Oct-06
Monoclonal Antibody to Leptin (human) (HLEP 55G)
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SYNONYMS anti-OB Gene Product (human) MAb (HLEP 55G)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-804-520-C050   50 µg 195.00 USD Add To Cart
ALX-804-520-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: HLEP 55G
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human leptin.
SPECIFICITY: Recognizes human leptin.
APPLICATION: ELISA (direct and indirect: 1:5'000-1:10'000)
Western Blot (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-701 Revised 31-Oct-06
Monoclonal Antibody to Leptin (rat) (RLEP 227)
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SYNONYMS anti-OB Gene Product (rat) MAb (RLEP 227)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-804-701-C050   50 µg 195.00 USD Add To Cart
ALX-804-701-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE: RLEP 227
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant rat leptin.
SPECIFICITY: Recognizes both natural and recombinant rat leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with human or mouse leptin.
APPLICATION: ELISA (direct and indirect: 1:5'000-1:10'000).
Western Blot (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-702 Revised 31-Oct-06
Monoclonal Antibody to Leptin (human) (HLEP 155)
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SYNONYMS anti-OB Gene Product (human) MAb (HLEP 155)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-702-C050   50 µg 195.00 USD Add To Cart
ALX-804-702-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: HLEP 155
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human leptin.
SPECIFICITY: Recognizes both natural and recombinant human leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with mouse or rat leptin.
APPLICATION: ELISA (direct and indirect: 1:5'000-1:10'000).
Western Blot (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After receipt, prepare aliquots and store at -20°C.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-105 Revised 28-Sep-06
Polyclonal Antibody to Leptin
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SYNONYMS anti-OB Gene Product PAb
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-210-105-R050   50 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.7mg/ml
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 25-44 (Q25KVQDDTKTLIKTIVTRIND44) of mouse leptin.
SPECIFICITY: Recognizes human, mouse, rat and sheep leptin.
APPLICATION: Immunocytochemistry (1:1'000)
Immunohistochemistry (frozen sections (1:1'000))
Western Blot (1:4'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-166 Revised 13-Dec-04
Polyclonal Antibody to Leptin
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SYNONYMS anti-OB Gene Product PAb
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-210-166-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Purified IgG in PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 91-106 (S91RNVIQIS
NDLENLRD106) of human leptin.
SPECIFICITY: Recognizes human, mouse and pig leptin. Detects a band of ~16kDa by Western blot.
APPLICATION: Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure.
Ob knockout mice are characterized by several metabolic abnormalities including, hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-168 Revised 07-May-07
Polyclonal Antibody to Leptin Receptor
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SYNONYMS anti-OB-R PAb
anti-CD295 PAb
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Leptin / Related Products
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ALX-210-168-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 577-594 (K577EIQWKTHEVFDAKSKSA594) of the extracellular domain of rat leptin receptor.
SPECIFICITY: Recognizes human, mouse and rat leptin receptor. Detects bands of ~100kDa and ~125kDa by Western blot, representing the short and long forms of leptin receptor. Also detects two unrelated bands of ~31kDa and ~35kDa.
APPLICATION: Western Blot (1:2'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-161-003.
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Product Specific Literature References
A leptin dose response study in obese (ob/ob) and lean (+/?) mice: R.B.S. Harris, et al.; Endocrinology 139, 8 (1998)