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Obesity & Adipokines
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ALX-804-252 Revised 22-Oct-07
Monoclonal Antibody to TNF-α (mouse) (MP6-XT3)
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SYNONYMS anti-Tumor Necrosis Factor-α (mouse) MAb (MP6-XT3)
anti-TNFSF 2 (mouse) (MP6-XT3)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-252-C100   100 µg 264.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: MP6-XT3
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes mouse TNF-α.
APPLICATION: ELISA
Western Blot (1-5µg/ml)
Functional Application (Neutralization)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-199 Revised 18-Sep-07
Monoclonal Antibody to TNF-α (human) (TNF-D)
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SYNONYMS anti-Tumor Necrosis Factor-α (human) MAb (TNF-D)
anti-TNFSF 2 (human) MAb (TNF-D)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-199-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: TNF-D
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Recombinant human TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes human TNF-α. Does not cross-react with mouse TNF-α or human or mouse TNF-β.
APPLICATION: ELISA: Use as capture or detection antibody in sandwich ELISA.
Flow Cytometry: Suitable as primary antibody for staining in FACS analysis; recommended concentration is 10µg/ml as determined on PBMC. Method: PBMC were incubated with PMA, ionomycin, and with  monensin to inhibit secretion. Incubation for 20 minutes with TNF-D at 10µg/ml; stained with goat-anti-mouse IgG-FITC second step reagent for 15 minutes.
Functional Application: Selectively neutralizes human TNF-α bioactivity. TNF-D requires an incubation of antigen and antibody for 1 hour at 37 °C or 16 hours at 4 °C to completely neutralize TNF-α cytolytic activity.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening aliquot contents and freeze at -20°C.
Product Specific Literature References
Monoclonal antibodies to human tumor necrosis factors alpha and beta: application for affinity purification, immunoassays, and as structural probes: T.S. Bringman & B.B. Aggarwal; Hybridoma 6, 489 (1987) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-371 Revised 01-Jun-06
Monoclonal Antibody to TNF-α (mouse) (1F3F3D4 IGH 156)
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SYNONYMS anti-TNFSF 2 (mouse) MAb (1F3F3D4IGH 156)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-371-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 1F3F3D4 IGH 156
ISOTYPE: Rat IgM
CONCENTRATION: 0.1mg/ml
PURITY: >95% (SDS-PAGE).
FORMULATION: Liquid. Antibody in PBS.
IMMUNOGEN: Recombinant human TNF-α (tumor necrosis factor-α).
SPECIFICITY: Recognizes mouse TNF-α.
APPLICATION: ELISA
Functional Application (neutralizing)
: 25ng/ml neutralizes the bioactivity of 40pg/ml recombinant mouse TNF-α by >95%.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-200 Revised 04-Apr-06
Monoclonal Antibody to TNF-R1 (human) (H398)
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SYNONYMS anti-TNF-R 60kDa (human) MAb (H398)
anti-p55 (human) MAb (H398)
anti-CD120a (human) MAb (H398)
anti-TNFRSF 1A (human) MAb (H398)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R1
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ALX-804-200-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: H398
ISOTYPE: Mouse IgG2a
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Human TNF-R1.
SPECIFICITY: Recognizes membrane-bound and soluble human TNF-R1. Does not cross-react with mouse TNF-R1.
APPLICATION: Flow Cytometry
Immunohistochemistry (frozen sections): Stains acetone-fixed cryostat sections or cell smears. Recommended for H398 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP), or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. H398 may be used at a concentration of 1-0.5mg/ml for cryostat sections.
Not recommended for paraffin sections.
Functional Application: Blocks TNF-R signalling. 10µg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%). Furthermore, 10µg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%).
TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells. Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of a 60-kD tumor necrosis factor (TNF) receptor as the major signal transducing component in TNF responses: B. Thoma, et al.; J. Exp. Med. 172, 1019 (1990) Abstract
Monoclonal antibodies to soluble human TNF receptor (TNF binding protein) enhance its ability to block TNF toxicity: G.R. Adolf & B. Fruhbeis; Cytokine 4, 180 (1992) Abstract
Inhibition of tumor necrosis factor (TNF)-mediated NF-kappa B activation by selective blockade of the human 55-kDa TNF receptor: G. Kruppa, et al.; J. Immunol. 148, 3152 (1992) Abstract
TR60 and TR80 tumor necrosis factor (TNF)-receptors can independently mediate cytolysis: M. Grell, et al.; Lymphokine Cytokine Res. 12, 143 (1993) Abstract
Cytostatic and cytotoxic effects of tumor necrosis factor alpha on MCF-7 human breast tumor cells are differently inhibited by glucocorticoid hormones: M.C. Pagliacci, et al.; Lymphokine Cytokine Res. 12, 439 (1993) Abstract
Lymphotoxin acts as an autocrine growth factor for Epstein-Barr virus-transformed B cells and differentiated Burkitt lymphoma cell lines: D.L. Gibbons, et al.; Eur. J. Immunol. 24, 1879 (1994) Abstract
TNF-induced superoxide anion production in adherent human neutrophils involves both the p55 and p75 TNF receptor: J. Richter, et al.; J. Immunol. 154, 4142 (1995) Abstract
Gastrin secretion from primary cultures of rabbit antral G cells: stimulation by inflammatory cytokines: N. Weigert, et al.; Gastroenterology 110, 147 (1996) Abstract
1alpha,25-dihydroxyvitamin D3 induces sphingomyelin hydrolysis in HaCaT cells via tumor necrosis factor alpha: C.C. Geilen, et al.; J. Biol. Chem. 272, 8997 (1997) Abstract; Full Text
Lipopolysaccharide mediates endothelial apoptosis by a FADD-dependent pathway: K.B. Choi, et al.; J. Biol. Chem. 273, 20185 (1998) Abstract; Full Text
Cycloheximide-induced T-cell death is mediated by a Fas-associated death domain-dependent mechanism: D. Tang, et al.; J. Biol. Chem. 274, 7245 (1999) Abstract; Full Text
Interferon-gamma rescues TNF-alpha-induced apoptosis mediated by up-regulation of TNFR2 on EoL-1 cells: T. Horie, et al.; Exp. Hematol. 27, 512 (1999) Abstract
Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand: G. Lu, et al.; J. Immunol. 168, 1831 (2002) Abstract
Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy: C. Gajate & F. Mollinedo; J. Biol. Chem. 280, 11641 (2005) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-450 Revised 26-Jul-07
Monoclonal Antibody to TNF-R2 (80M2)
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SYNONYMS anti-CD120b MAb (80M2)
anti-p75 MAb (80M2)
anti-TNF-R 80kDa MAb (80M2)
anti-TNFRSF 1B (80M2)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R2
Ordering Information
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ALX-804-450-C100   100 µg 590.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: 80M2
ISOTYPE: Mouse IgG1
CONCENTRATION: 100µg/ml
FORMULATION: Liquid. 0.2µm-filtered solution in PBS containing 0.1% BSA.
IMMUNOGEN: Human TNF-R2.
SPECIFICITY: Recognizes the extracellular part of human and rat TNF-R2 and soluble human TNF-R2.
APPLICATION: Flow Cytometry
Immunohistochemistry (frozen sections; not suitable for paraffin sections)
Immunoprecipitation
Western Blot
Functional Application (non-agonistic receptor modulation: enhances in vitro TNF responses by increasing the affinity of soluble TNF-α to TNF-R2).
Optimal conditions must be determined individually for each application. Typical starting dilution is 1:10.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
POSITIVE CONTROL: Human lymph nodes can be used as positive control for immunohistochemistry, PHA activated T cells for flow cytometry.
General Information
BACKGROUND/TECHNICAL INFORMATION The reactivity of the antibody with cell-bound TNF-Receptor is minimally inhibited by high concentrations of TNF-α.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-449 Revised 03-Nov-06
Monoclonal Antibody to TNF-R2 (MR2-1)
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SYNONYMS anti-CD120b MAb (MR2-1)
anti-p75 MAb (MR2-1)
anti-TNF-R 80kDa MAb (MR2-1)
anti-TNFRSF 1B MAb (MR2-1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R2
Ordering Information
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ALX-804-449-C100   100 µg 590.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: MR2-1
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.1mg/ml
FORMULATION: Liquid. 0.2µm-filtered antibody in PBS containing 0.1% BSA and 0.02% sodium azide.
IMMUNOGEN: Human TNF-R2.
SPECIFICITY: Recognizes the extracellular part of human TNF-R2 and soluble human TNF-R2. Cross-reacts with rhesus and cynomolgus natural TNF-R2.
APPLICATION: Flow Cytometry
Immunohistochemistry
(frozen sections, not paraffin sections)
Immunoprecipitation
Western Blot
Functional Application
(agonistic (after cross-linking) and receptor modulating).
Optimal conditions must be determined individually for each application. Recommended starting dilution is 1:10.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
BACKGROUND/TECHNICAL INFORMATION The reactivity of the antibody with cell-bound TNF-Receptor is minimally inhibited by high concentrations of TNF-α.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-730 Revised 10-Apr-08
Monoclonal Antibody to Vaspin (human) (VP63)
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SYNONYMS anti-Visceral Adipose Tissue-derived Serine Protease Inhibitor (human) MAb (VP63)
anti-Serpin A12 (human) MAb (VP63)
anti-OL-64 (human) MAb (VP63)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Vaspin/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-730-C050   50 µg 195.00 USD Add To Cart
ALX-804-730-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: VP63
ISOTYPE: Mouse IgG1κ
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human vaspin.
SPECIFICITY: Recognizes human vaspin.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:5’000)
Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-052 Revised 07-Mar-06
Monoclonal Antibody to Viable Rat beta Cells and RIN Cells (K14D10)
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Insulin & Insulin Receptor/Related Products
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ALX-803-052-C100   100 µg 547.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE: K14D10
ISOTYPE: Mouse IgG2a
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Ammonium sulphate precipitation.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.9mg/ml sodium azide.
IMMUNOGEN: Pig proinsulin.
SPECIFICITY: Recognizes surface structure of viable isolated rat beta cells. Does not recognize mouse islet beta cells. Cross-reactivity with human pancreatic beta cells was not detected.
APPLICATION: Flow Cytometry
Immunohistochemistry (frozen sections)
Note: Not recommended for WB and IP.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Influence of target cell preparation on binding of monoclonal islet cell reactive antibodies (mc-ICRA) in cellular enzyme-linked immunosorbent assay (CELISA): M. Schlosser, et al.; J. Immunol. Methods 140, 101 (1991) Abstract
Monoclonal antibody-mediated cytotoxicity against rat beta cells detected in vitro does not cause beta-cell destruction in vivo: B. Ziegler, et al.; Diabetologia 35, 608 (1992) Abstract
Co-culture of pancreatic islets and allogeneic lymphocytes: alterations of responder and stimulator cells: B. Kuttler, et al.; Transplantation 64, 480 (1997) Abstract
Recognition of islet-directed peripheral blood lymphocytes in vitro before rejection of allogeneic grafted islets: B. Kuttler, et al.; Transplantation 69, 1987 (2000) Abstract
Immunomagnetic purification of beta cells from rat islets of Langerhans: V. Hadjivassiliou, et al.; Diabetologia 43, 1170 (2000) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-201-319 Revised 24-Jun-08
Nampt/Visfatin (human) (recombinant) (His)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (human) (recombinant) (His)
PBEF1 (human) (recombinant) (His)
Pre-B-Cell Colony Enhancing Factor 1 (human) (recombinant) (His)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-319-C050   50 µg 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Full length human Nampt/visfatin (aa 1-491) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Salt-free.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL test)
RECONSTITUTION: Reconstitute with an appropriate buffer.
BIOLOGICAL ACTIVITY: Shows adipogenic effects in differentiating 3T3-L1 cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Working aliquots are stable for up to 3 months when stored at -20°C.

HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) Abstract
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P43490: Pre-B-Cell Colony Enhancing Factor 1 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins/Fusion ProteinsNAD+ Metabolism/Related Products
 
 
ALX-201-336 Revised 24-Jun-08
Nampt/Visfatin (human) (recombinant)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (human) (recombinant)
PBEF1 (human) (recombinant)
Pre-B-Cell Colony Enhancing Factor 1 (human) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-336-C010   10 µg 295.00 USD Add To Cart
ALX-201-336-C050   50 µg 795.00 USD Add To Cart