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Obesity & Adipokines
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AG-N0823 Revised 17-Jun-08 New product
Nampt/Visfatin (human/rat) (IntraCellular) Dual ELISA Kit
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-N0823EK-KI01   96 wells 640.00 USD Add To Cart
AG-N0823TP-KI01 Twin Plex 2x96 wells 940.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SENSITIVITY: 30pg/ml (range 0 to 32ng/ml)
SPECIFICITY: Does not cross-react with mouse sera.
APPLICATION: For the quantitative determination of Nampt/Visfatin in human or rat cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
ELISA & EIA Kits • NAD+ Metabolism/Related Products
 
 
AG-VN0812 Revised 07-Jul-08 New product
Nampt/Visfatin (mouse) ELISA Kit
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse) ELISA Kit
PBEF1 (mouse) ELISA Kit
Pre-B-Cell Colony Enhancing Factor 1 (mouse) ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-VN0812EK-KI01   96 wells 660.00 USD Add To Cart
AG-VN0812TP-KI01 Twin Plex 2x96 wells 920.00 USD Add To Cart
AG-VN0812PP-KI01 Penta Plex 5x96 wells 2'050.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SENSITIVITY: 50pg/ml (range 0 to 64ng/ml)
SPECIFICITY: Does not cross-react with human or rat Nampt/visfatin.
APPLICATION: For the quantitative determination of mouse Nampt/visfatin in serum.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
ELISA & EIA Kits • NAD+ Metabolism/Related Products
 
 
ALX-270-102 Revised 10-Aug-07
Naproxen
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SYNONYMS (S)-6-Methoxy-α-methyl-2-naphthalene acetic acid
PRODUCT LINE Inflammation
PRODUCT CATEGORY Non-Steroidal Anti-Inflammatory Drugs [NSAIDS]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-102-G005   5 g 35.00 USD Add To Cart
Product Specification
FORMULA: C14H14O3
MW: 230.3
CAS NUMBER: 22204-53-1
MERCK INDEX: 14: 6417
PURITY: ≥98% (Assay)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO, dimethyl formamide and 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: MAY BE MUTAGENIC. HARMFUL. MAY BE TERATOGENIC.

Product Description
Cyclooxygenase (COX) inhibitor. Widely used non-steroidal anti-inflammatory drug.
Product Specific Literature References
Perspectives in nonsteroidal anti-inflammatory agents: T.Y. Shen; Angew. Chem. Int. Ed. 11, 460 (1972) Abstract
Further Categories Containing This Product:
PPAR Agonists • COX Inhibitors
 
 
ALX-522-112 Revised 27-Mar-08
Nesfatin-1 (mouse) (recombinant)
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Food Intake Modulators Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-112-C010   10 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
MW: ~8kDa
SOURCE/HOST: Recombinant mouse nesfatin-1 (aa 25-106) produced in E. coli.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 0.2% bovine serum albumin (BSA).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor]
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006) Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-114 Revised 27-Mar-08
Nesfatin-1 (mouse) (recombinant) (tagged)
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Food Intake Modulators Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-114-C050   50 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
MW: ~35kDa
SOURCE/HOST: Produced in E. coli. Recombinant mouse nesfatin-1 (aa 25-106) is fused at the N-terminus to a GST-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 0.2% bovine serum albumin (BSA).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor]
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006) Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-116 Revised 27-Mar-08
Nesfatin-1 (rat) (recombinant)
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Food Intake Modulators Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-116-C010   10 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
MW: ~8kDa
SOURCE/HOST: Recombinant rat nesfatin-1 (aa 25-106) produced in E. coli.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 0.2% bovine serum albumin (BSA).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9JI85: Nucleobindin-2 (rat) [Precursor]
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006) Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-118 Revised 27-Mar-08
Nesfatin-1 (rat) (recombinant) (tagged)
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Food Intake Modulators Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-118-C050   50 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
MW: ~35kDa
SOURCE/HOST: Produced in E. coli. Recombinant rat nesfatin-1 (aa 25-106) is fused at the N-terminus to a GST-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 0.2% bovine serum albumin (BSA).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behaviour. Intracerebroventricular injection of nesfatin-1 reduces body weight in rat by affecting the animal food intake.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9JI85: Nucleobindin-2 (rat) [Precursor]
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of nesfatin-1 as a satiety molecule in the hypothalamus: I.S. Oh, et al.; Nature 443, 709 (2006) Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
APO-54N-036 Revised 19-Jun-08 New product
Nesfatin-1 (mouse/rat) Detection Set [For ELISA Application]
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Food Intake Modulators Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-036-KI01   1 Set 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SENSITIVITY: 0.3ng/ml (range 0 to 16ng/ml).
QUANTITY: For 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of mouse or rat nesfatin-1 from biological samples.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
Brain hypothalamus expressed several secreted molecules that function in regulating feeding behavior [1]. NUCB2 (NUCB2 also called NEFA for DNA binding /EF-hand /acidic protein) is a hypothalamus-secreted protein containing 396 amino acids that is highly conserved in human, mice and rat [2, 3]. NUCB2 when injected directly into the brain of rats promotes anorexia and decreases body weight [3]. NUCB2 is cleaved posttranslationally by prohormone convertases into a N-terminus-fragment Nesfatin-1 and  two C-terminal peptides, Nesfatin-2 and Nesfatin-3 [3]. Nesfatin-1 possesses all of the anorexigenic property of NUCB2 [4]. The conversion of NUCB2 into Nesfatin-1 is indispensable for its activity in vivo [3].  Nesfatin-1 is found in discrete nuclei of the hypothalamus where it probably activates a leptin-independent melanocortin pathway [3]. Nesfatin-1 crosses the Blood Brain Barrier (BBB) in both the blood-to-brain and brain-to-blood directions by a nonsaturable system [5].  NUCB2 is also expressed in the adipocyte cell line 3T3L1 suggesting other functions of Nesfatin-1 outside brain or peripheral source of Nesfatin-1 affecting brain function [6]. Nesfatin-1 in rat stimulates calcium influx and interacts with a G protein-coupled receptor still to be characterized [7].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P81117: Nucleobindin-2 (mouse) [Precursor], Q9JI85: Nucleobindin-2 (rat) [Precursor]
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Hormonal regulation of food intake: S. Stanley, et al.; Physiol. Rev. 85, 1131 (2005) Abstract; Full Text
[2] Isolation, characterization, and primary structure of a calcium-binding 63-kDa bone protein: M. Wendel, et al.; JBC 270, 6125 (1995) Abstract; Full Text
[3] Identification of nesfatin-1 as a satiety molecule in the hypothalamus: S. Oh-I, et al.; Nature 443, 709 (2006) Abstract
[4] Serotonin systems upregulate the expression of hypothalamic NUCB2 via 5-HT2C receptors and induce anorexia via a leptin-independent pathway in mice: K. Nonogaki, et al.; BBRC 372, 186 (2008)