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ALX-201-082
Revised 07-Feb-06
Procaspase-3 (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-082-C005
5 µg
413.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Requires activation.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 0.1mg/ml with PBS containing 15% glycerol.
APPLICATION:
Very useful in studying mechanisms of caspase activation and regulation, as well as in investigating cascades of caspase-3 mediated signal transduction pathways.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C
General Information
Procaspase-3 (human) (recombinant) is highly activatable by treatment with active human recombinant caspase-8 (Prod. No.
ALX-201-062
). Activation is routinely tested using 10-30ng of procaspase-3 treated with 0.-0.5 units of the active recombinant caspase-8 in 100µl of reaction buffer for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric (Prod. No.
ALX-850-216
) and Colorimetric Assay Kits (Prod. No.
ALX-850-215
).
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-164
Revised 07-Feb-06
Procaspase-9 (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-164-C050
50 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli.
Requires activation.
CONCENTRATION:
0.78mg/ml
PURITY:
~90% (SDS-PAGE)
FORMULATION:
Liquid. In 20mM HEPES, pH 7.4, containing 10mM KCl, 1.5mM MgCl
2
, 1mM DTT, 1mM EDTA, 1mM EGTA, 0.1mM PMSF and 50% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stability at -20°C: 1-2 weeks.
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-165
Revised 07-Feb-06
Procaspase-9 (mouse) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-165-C050
50 µg
280.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli.
Requires activation.
CONCENTRATION:
0.57mg/ml
PURITY:
~90% (SDS-PAGE)
FORMULATION:
Liquid. In 20mM TRIS-HCl, pH 8.5.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stability at -20°C: 1-2 weeks.
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-056
Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
SYNONYMS
Interleukin 1
β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-056-U025
25 U
231.00 USD
ALX-201-056-U100
100 U
385.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-
p
NA (
p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-
p
NA (Prod. No.
ALX-260-026
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P29466:
Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492:
Caspase-1 (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Inflammasome
•
Enzymes
ALX-201-057
Revised 30-Jun-08
Caspase-2 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-057-U025
25 U
203.00 USD
ALX-201-057-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥
95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
10'000-13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VDVAD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening capase inhibitors, studying enzyme regulation and kinetics, determining specificity of capase substrates, or as positive control in caspase activity assays.
We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to Caspase-2 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-214
or
ALX-850-213
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-2 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kDa) and small (12kDa) subunits which associate to form the active enzyme. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA (Prod. No.
ALX-260-059
). The signal can be detected by spectrophotometry.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P42575:
Caspase-2 (human) (precursor)
AfCS Siganlling Gateway link A000497:
Caspase-2 (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-135
Revised 24-Jan-05
Caspase-2 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-135-C005
5 µg
255.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Liquid. 5µg in 25µl of 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for at least one week when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Substrate specificities of caspase family proteases
:
R.V. Talanian, et al.; J. Biol. Chem.
272
, 9677 (1997)
Abstract
;
Full Text
General Information
When expressed in
E. coli
, caspase-2 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-2 enzymatic activity can be measured by the release of AFC from the caspase substrate Ac-VDVAD-AFC (Prod. No.
ALX-260-112
) as emission at 505nm and excitation at 400nm using a spectrofluorometer.
BACKGROUND/TECHNICAL INFORMATION
Enzyme Evaluation Protocol
This protocol is used to measure caspase enzyme activity. The synthetic fluorogenic peptide Ac-VDVAD-AFC (Prod. No.
ALX-260-112
) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AFC (7-amino-4-trifluoromethylcoumarin). AFC release can be measured by spectrofluorometry using UV.
1. Add 20mM of Ac-VDVAD-AFC (Prod. No.
ALX-260-112
) to 1ml of assay buffer (20mM PIPES, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose, pH 7.2). Add the appropriate amount of active caspase to the mixture (50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AFC liberated from the Ac-VDVAD-AFC using a spectrofluorometer with an excitation wavelength of 400nm and an emission wavelength of 505nm.
Note:
Enzyme activity can also be measured using the caspase enzyme substrate Ac-VDVAD-pNA (Prod. No.
ALX-260-059
) at 37°C in a spectromax at 405nm wavelength.
Swiss-Prot link P42575:
Caspase-2 (human) (precursor)
AfCS Signalling Gateway link A000497:
Caspase-2 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-522-083
Revised 24-Mar-05
Caspase-2 (active) (human) (recombinant) (high purity)
SYNONYMS
ICH-1 (active) (human) (recombinant) (high purity)
CASP-2 (active) (human) (recombinant) (high purity)
ICH-1 protease (active) (human) (recombinant) (high purity)
ICH-1L/1S (active) (human) (recombinant) (high purity)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-522-083-C005
5 µg
180.00 USD
Product Specification
MW:
~31kDa; α-subunit: ~19kDa; β-subunit: ~12kDa.
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli.
CONCENTRATION:
0.072mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. 5µg of recombinant protein in 20mM HEPES, pH 7.5, containing 4mM DTT, 10% sucrose (w/v) and 150mM NaCl.
SPECIFIC ACTIVITY:
~80'000U/mg. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate Ac-VDVAD-pNA (Prod. No.
ALX-260-059
)
per hour
at 37°C in a reaction solution containing 100mM MES, pH 6.5, 10% PEG 600, 10mM DTT and 0.1% CHAPS.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Crystal structure of caspase-2, apical initiator of the intrinsic apoptotic pathway
:
A. Schweizer, et al.; J. Biol. Chem.
278
, 42441 (2003)
Abstract
;
Full Text
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-038
Revised 24-Jan-05
Caspase-3 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-038-C005
5 µg
255.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Liquid. 5µg in 25µl of 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for at least one week when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
[1]
Biochemical characteristics of caspases-3, -6, -7 and -8:
H.R. Stennicke & G.S. Salvesen; J. Biol. Chem.
272
, 25719 (1997)
Abstract
;
Full Text
General Information
When expressed in
E. coli
, caspase-3 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-3 enzymatic hydrolysis can be measured by the release of AMC from the caspase substrate Ac-DEVD-AMC (Prod.No.
ALX-260-031
) as emission at 440nm and excitation at 380nm using a spectrofluorometer [1].
BACKGROUND/TECHNICAL INFORMATION
Enzyme Evaluation Protocol
This protocol is used to measure caspase enzyme activity [1]. The synthetic fluorogenic peptide Ac-DEVD-AMC (Prod. No.
ALX-260-031
) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC (7-amino-4-methylcoumarin). AMC release can be measured by spectrofluorometry using UV.
1. Add 20mM of Ac-DEVD-AMC (Prod. No.
ALX-260-031
) to 1ml of assay buffer (20mM PIPES, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose, pH 7.2). Add the appropriate amount of active caspase to the mixture (50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AMC liberated from the Ac-DEVD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm.
Note:
Enzyme activity can also be measured using the caspase enzyme substrate Ac-DEVD-pNA (Prod. No.
ALX-260-033
) at 37°C in a spectromax at 405nm wavelength.
Swiss-Prot link P42574:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link A000498:
Caspase-3 (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-059
Revised 21-Jul-08
Caspase-3 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-059-U025
25 U
119.00 USD
ALX-201-059-U100
100 U
273.00 USD
Product Specification
EC:
3.4.22.56
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
300’000-400’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA (Prod. No.
ALX-260-033
) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-216
or
ALX-850-215
).
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-
p
NA (Prod. No.
ALX-260-033
) and Ac-DEVD-AFC (Prod. No.
ALX-260-032
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.
BACKGROUND/TECHNICAL INFORMATION
Swiss Prot link:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link:
Caspase-3 (mouse)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-522-069
Revised 30-Jun-05
Caspase-3 (active) (human) (recombinant) (high purity)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-522-069-C005
5 µg
180.00 USD
Product Specification
MW:
~29kDa; α-subunit: ~17kDa; β-subunit: ~12kDa.
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli.
CONCENTRATION:
0.1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 20mM PIPES, pH 7.4, containing 10mM DTT, 100mM sodium chloride, 10% (w/v) sucrose, 0.1% (w/v) CHAPS and 1mM EDTA.
SPECIFIC ACTIVITY:
≥50'000U/mg.
One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate Ac-DEVD-AMC (Prod. No.
ALX-260-031
) per min. at 37°C in a buffered solution.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
The rate of recombinant human caspase-3 enzymatic activity can be measured by the release of AMC from the Ac-DEVD-AMC (Prod. No.
ALX-260-031
) caspase substrate as emission at 440nm upon excitation at 380nm using a spectrofluorometer [1].
Swiss-Prot link:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link:
Caspase-3 (mouse)
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
[1]
Biochemical characteristics of caspases-3, -6, -7, and -8
:
H.R. Stennicke & G.S. Salvesen; J. Biol. Chem.
272
, 25719 (1997)
Abstract
;
Full Text
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Enzymes
ALX-201-162
Revised 08-Dec-04
Caspase-3 (active) (mouse) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
