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ALX-804-717 Revised 24-Jun-08
Monoclonal Antibody to Nampt/Visfatin (OMNI379)
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SYNONYMS anti-Nicotinamide Phosphoribosyltransferase MAb (OMNI379)
anti-PBEF1 MAb (OMNI379)
anti-Pre-B-Cell Colony Enhancing Factor 1 MAb (OMNI379)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-717-C050   50 µg 195.00 USD Add To Cart
ALX-804-717-C100   100 µg 270.00 USD Add To Cart
ALX-804-717B-C050 Biotin 50 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: OMNI379
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human Nampt/visfatin.
SPECIFICITY: Recognizes human, mouse and rat Nampt/visfatin. Detects a band of ~54kDa by Western blot.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:5’000)
Immunohistochemistry (paraffin sections (1:500-1:1'000))
Western Blot (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006) Abstract
Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) Abstract; Full Text
Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
NAD+ Metabolism/Related Products • Monoclonal Antibodies
 
 
ALX-201-235 Revised 17-Aug-06
NAD Kinase (full length) (active) (human) (recombinant) (His)
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SYNONYMS NADK (full length) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (full length) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-235-C050   50 µg 320.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK is fused at the N-terminus to a His-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Enzymes • Kinases Other Products • Recombinant Proteins/Fusion Proteins
 
 
ALX-201-236 Revised 17-Aug-06
NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
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SYNONYMS NADK (NT truncated) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-236-C050   50 µg 260.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK (aa 64-446) is fused at the N-terminus to a His-tag.
QUANTITY: Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Liquid. Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SPECIFIC ACTIVITY: ≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Enzymes • Kinases Other Products • Recombinant Proteins/Fusion Proteins
 
 
ALX-480-003 Revised 10-Dec-04
NADP . disodium salt
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SYNONYMS β-Nicotinamide Adenine Dinucleotide Phosphate
β-NADP
Coenzyme II
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-480-003-M050   50 mg 20.00 USD Add To Cart
ALX-480-003-M250   250 mg 55.00 USD Add To Cart
ALX-480-003-G001   1 g 160.00 USD Add To Cart
Product Specification
FORMULA: C21H26N7O17P3 . 2Na
MW: 741.4 . 46.0
CAS NUMBER: 1184-16-3
MERCK INDEX: 14: 6348
PURITY: ≥97%
APPEARANCE: Light yellow powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry. Protect from light.

Further Categories Containing This Product:
Adenosine Derivatives Other Products
 
 
ALX-480-004 Revised 06-Aug-08
NADPH . tetrasodium salt
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SYNONYMS β-Nicotinamide Adenine Dinucleotide Phosphate (reduced form) . 4Na
β-NADPH . 4Na
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-480-004-M050   50 mg 58.00 USD Add To Cart
ALX-480-004-M250   250 mg 190.00 USD Add To Cart
ALX-480-004-G001   1 g 580.00 USD Add To Cart
Product Specification
FORMULA: C21H26N7O17P3 . 4Na
MW: 741.4 . 92.0
CAS NUMBER: 2646-71-1
PURITY: ≥95% (HPLC)
APPEARANCE: White to light yellow powder.
SOLUBILITY: Soluble in water (50mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Further Categories Containing This Product:
Adenosine Derivatives Other Products
 
 
ALX-201-319 Revised 24-Jun-08
Nampt/Visfatin (human) (recombinant) (His)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (human) (recombinant) (His)
PBEF1 (human) (recombinant) (His)
Pre-B-Cell Colony Enhancing Factor 1 (human) (recombinant) (His)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-319-C050   50 µg 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Full length human Nampt/visfatin (aa 1-491) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Salt-free.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL test)
RECONSTITUTION: Reconstitute with an appropriate buffer.
BIOLOGICAL ACTIVITY: Shows adipogenic effects in differentiating 3T3-L1 cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) Abstract
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P43490: Pre-B-Cell Colony Enhancing Factor 1 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • NAD+ Metabolism/Related Products
 
 
ALX-201-336 Revised 30-Jul-08
Nampt/Visfatin (human) (recombinant)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (human) (recombinant)
PBEF1 (human) (recombinant)
Pre-B-Cell Colony Enhancing Factor 1 (human) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-336-C010   10 µg 295.00 USD Add To Cart
ALX-201-336-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Full length human Nampt/visfatin (aa 1-491) is fused at the N-terminus to a FLAG®-tag.
PURITY: ≥90% (SDS-PAGE)
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
BIOLOGICAL ACTIVITY: Shows adipogenic effects in differentiating 3T3-L1 cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Product Images
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General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P43490: Pre-B-Cell Colony Enhancing Factor 1 (human)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • NAD+ Metabolism/Related Products
 
 
ALX-201-364 Revised 24-Jun-08
Nampt/Visfatin (mouse) (recombinant)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse) (recombinant)
PBEF1 (mouse) (recombinant)
Pre-B-Cell Colony Enhancing Factor 1 (mouse) (recombinant)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin/Nampt/PBEF/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-364-C010   10 µg 295.00 USD Add To Cart
ALX-201-364-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Full length mouse Nampt/visfatin (aa 1-491) is fused at the N-terminus to a FLAG®-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot links Q9JKM0 & Q99KQ4: Pre-B-Cell Colony Enhancing Factor 1 (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins • NAD+ Metabolism/Related Products