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Protein Synthesis, Modification & Degradation
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ALX-804-391 Revised 02-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (10D8)
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SYNONYMS anti-GlcNAc MAb (10D8)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-391-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 10D8
ISOTYPE: Mouse IgM
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: N-Acetylglucosamine.
SPECIFICITY: Recognizes human, mouse and rat N-acetylglucosamine.
APPLICATION: Western Blot (1 µg/ml for AP/BCIP/NBT (MTT) detection; 0.5 µg/ml for HRPO/ECL detection).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-276 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (HGAC85)
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SYNONYMS anti-Glc-NAc (O-linked) MAb (HGAC85)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-276-C100   100 µg 379.00 USD Add To Cart
Product Specification
CLONE: HGAC85
ISOTYPE: Mouse IgG3
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Heat killed, pepsin treated group A streptococci.
SPECIFICITY: Recognizes β-1,3 linked O-GlcNAc (O-linked N-acetylglucosamine) residues of streptococcal group A carbohydrates as well as O-GlcNAc glycosylated proteins.
APPLICATION: ELISA
Immunocytochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1µg/ml)
Note: Do not use with diluents containing glycosylated proteins!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier: X. Zhang & V. Bennett; J. Biol. Chem. 271, 31391 (1996) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-111 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)
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SYNONYMS anti-Glc-NAc (O-linked) MAb (RL2)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
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ALX-804-111-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: RL2
ISOTYPE: Mouse IgG1
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Pore complex-lamina fraction purified from rat liver nuclear envelopes.
SPECIFICITY: Recognizes human, mouse and rat nuclear pore complex (NPC), cytoplasmic and intracellular O-GlcNAc glycosylated proteins. Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (1:200))
Immunoprecipitation
Western Blot (1:1’000)
Note: Do not use with diluents containing glycosylated proteins.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins: C.M. Snow, et al.; J. Cell. Biol. 104, 1143 (1987) Abstract
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1: G. Majumdar, et al.; Diabetes 53, 3184 (2004) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-027 Revised 11-Jan-05
Monoclonal Antibody to α-Adaptin (AC1-M11)
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PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-027-R500   500 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Monkey
Others
CLONE: AC1-M11
ISOTYPE: Mouse IgG2a
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified adaptors.
SPECIFICITY: Recognizes human, mouse, rat, pig, monkey, bovine, chicken and frog α-adaptin. Recognizes the products of both α-adaptin genes, α-A and α-C as well as an alternatively spliced isoform of α-A found in neurons.
APPLICATION: Immunocytochemistry (1:100)
Immunoprecipitation
Western Blot (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies: M.S. Robinson; J. Cell. Biol. 104, 887 (1987) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-110 Revised 19-Oct-06
Monoclonal Antibody to α-Adaptin (AP6)
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SYNONYMS anti-AP2 MAb (AP6)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
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ALX-804-110-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
Others
CLONE: AP6
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified adaptors.
SPECIFICITY: Recognizes human, mouse, rat, monkey, bovine and hamster α-adaptin. Recognizes the products of both α-adaptin genes α-A and α-C as well as an alternatively spliced isoform of α-A found in neurons. Detects a doublet band of ~100kDa by Western blot, representing the two isoforms of α-adaptin in rat brain, though α-C is the dominant form seen.
APPLICATION: Immunocytochemistry (1:1’000)
Immunoprecipitation
Western Blot (1:200)
Functional Application: Microinjection of cells with ALX-804-110 results in variable blocking of endocytosis.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-028 Revised 12-Jun-08
Monoclonal Antibody to ADP-ribosylation Factor (1D9)
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SYNONYMS anti-ARF MAb (1D9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY ADP Ribosylation Factor [ARF] / Related Products
Ordering Information
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ALX-804-028-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
CLONE: 1D9
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human ARF1 (ADP-ribosylation factor-1).
SPECIFICITY: Recognizes human, mouse, rat, dog and hamster ARF1, ARF3, ARF5 and ARF6. Detects a band of ~21kDa by Western blot.
APPLICATION: Immunocytochemistry
Immunohistochemistry (1:500)
Immunoprecipitation
Western Blot (1:500)
Note: Not suitable for neutralizing ARF in solution.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane: M.M. Cavenagh, et al.; J. Biol. Chem. 271, 21767 (1996) Abstract; Full Text
Further Categories Containing This Product:
Vesicles & Membrane Trafficking Other Products • Monoclonal Antibodies
 
 
ALX-804-108 Revised 18-Sep-07
Monoclonal Antibody to Ah Receptor Nuclear Translocator (2B10)
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SYNONYMS anti-ARNT MAb (2B10)
anti-HIF-1β MAb (2B10)
anti-Hypoxia-inducible Factor 1β MAb (2B10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
Ordering Information
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ALX-804-108-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 2B10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 771-789 (N771SYNNEEFPDLTMFPPFSE789) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY: Recognizes human, mouse and monkey ARNT. Detects a band of ~87kDa by Western blot.
APPLICATION: Immunocytochemistry (1:1'000)
Immunoprecipitation
Western Blot (1:4'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor translocator: Characterization of two monoclonal antibodies to the ARNT: N.G. Hord & G. Perdew; Mol. Pharmacol. 46, 618 (1994) Abstract
Oxygen tension modulates beta-globin switching in embryoid bodies: S. Bichet, et al.; FASEB J. 13, 285 (1999) Abstract; Full Text
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell Sci. 112, 1203 (1999) Abstract; Full Text
Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein-mediated transactivation potential in a dimer context: W.P. Long, et al.; J. Biol. Chem. 274, 12391 (1999) Abstract; Full Text
DNA methylation represses the expression of the human erythropoietin gene by two different mechanisms: H. Yin and K.L. Blanchard; Blood 95, 111 (2000) Abstract; Full Text
A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses: Y.S. Chun, et al.; J. Cell Sci. 114, 4051 (2001) Abstract; Full Text
Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression: T. Sanchez-Elsner, et al.; J. Biol. Chem. 276, 38527 (2001) Abstract; Full Text
Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia: H. Suzuki, et al.; Oncogene 20, 5779 (2001) Abstract; Full Text
Rescue of hypoxia-inducible factor-1alpha-deficient tumor growth by wild-type cells is independent of vascular endothelial growth factor: G. Höpfl, et al.; Cancer Res. 62, 2962 (2002) Abstract; Full Text
The arylhydrocarbon receptor (AhR), but not the AhR-nuclear translocator (ARNT), is increased in hearts of patients with cardiomyopathy: M.R. Mehrabi, et al.; Virchows Arch. 441, 481 (2002) Abstract; Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products • Monoclonal Antibodies
 
 
ALX-804-283 Revised 10-Mar-08
Monoclonal Antibody to AIB1 (human) (AX15.3)
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SYNONYMS anti-Amplified in Breast Cancer 1 (human) MAb (AX15.3)
anti-SRC-3 (human) MAb (AX15.3)
anti-Steroid Receptor Coactivator-3 (human) MAb (AX15.3)
anti-NCOA3 (human) MAb (AX15.3)
anti-Nuclear Receptor Coactivator 3 (human) MAb (AX15.3)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Receptor Coactivators / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-283-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: AX15.3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Purified antibody in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant human AIB1 (amplified in breast cancer 1) (aa 605-1294).
SPECIFICITY: Recognizes human AIB1. Detects a band of ~155kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1: D.O. Azorsa, et al.; Hybridoma 18, 28 (1999) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-400 Revised 20-Sep-07
Monoclonal Antibody to Akt (dephosphorylated) (11A11)
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SYNONYMS anti-PKB (dephosphorylated) MAb (11A11)
anti-Protein Kinase B (dephosphorylated) MAb (11A11)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
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ALX-804-400-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 11A11
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal (QFSYSA) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474.
SPECIFICITY: Recognizes human, mouse, rat and dog Ser472/473/474-dephosphorylated Akt (see technical note). Does not cross-react with Ser472/473/474-phosphorylated Akt.
APPLICATION: Immunohistochemistry
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:

Included (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481        Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479         Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-553 Revised 01-Dec-05
Monoclonal Antibody to Akt (5C10)
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SYNONYMS anti-PKB MAb (5C10)
anti-Protein Kinase B MAb (5C10)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-553-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 5C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal human Akt (protein kinase B; PKB).
SPECIFICITY: Recognizes a c-terminal epitope (aa 466-480) of phosphorylated and non-phosphorylated human, mouse, rat and dog Akt isoforms (see Technical Note below).
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms.Thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3 respectively.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2: L461LELDQRTHFPQFS474YSASIRE481         Swiss-Prot-Link: P31751
Akt3:     D461NERRPHFPQFS472YSASGRE479      Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-303 Revised 18-Sep-07
Monoclonal Antibody to Akt (19G7)
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SYNONYMS anti-PKB MAb (19G7)
anti-Protein Kinase B MAb (19G7)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY