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ALX-270-022
Revised 09-Jan-08
AEBSF . hydrochloride
SYNONYMS
4-(2-Aminoethyl)benzenesulfonylfluoride . HCl
Pefabloc
®
SC
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Proteases Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-270-022-M050
50 mg
40.00 USD
ALX-270-022-M250
250 mg
145.00 USD
ALX-270-022-G001
1 g
360.00 USD
Product Specification
FORMULA:
C
8
H
10
FNO
2
S . HCl
MW:
203.2 . 36.5
CAS NUMBER:
30827-99-7
PURITY:
≥98% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in water or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from moisture.
Product Description
Specific irreversible inhibitor of serine proteases including trypsin, chymotrypsin, plasmin, plasma kallikrein, and thrombin. Stable, non-toxic alternative to PMSF (Prod. No.
ALX-270-184
). Inhibitor of NAD(P)H oxidase. Activator of MAP kinases JNK and p38.
Product Specific Literature References
Irreversible enzyme inhibitors. 180. Irreversible inhibitors of the C'la component of complement derived from m-(phenoxypropoxy)benzamidine and phenoxyacetamide:
B.R. Baker & H.J. Cory; J. Med. Chem.
14
, 119 (1971)
Abstract
Serine protease inhibitors block priming of monocytes for enhanced release of superoxide:
P. Megyeri, et al.; Immunology
86
, 629 (1995)
Abstract
;
Full Text
Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF:
M. Citron, et al.; Neuron
17
, 171 (1996)
Abstract
Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor:
Y. Nakabo & M.J. Pabst; J. Leukoc. Biol.
60
, 328 (1996)
Abstract
;
Full Text
Inhibition of NADPH oxidase activation by 4-(2-aminoethyl)-benzenesulfonyl fluoride and related compounds:
V. Diatchuk, et al.; J. Biol. Chem.
272
, 13292 (1997)
Abstract
;
Full Text
4-(2-Aminoethyl)benzenesulfonyl fluoride attenuates tumor-necrosis-factor-alpha-induced blood-brain barrier opening:
P. Megyeri, et al.; Eur. J. Pharmacol.
374
, 207 (1999)
Abstract
Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis:
R. Buitrago-Rey, et al.; J. Antimicrob. Chemother.
49
, 871 (2002)
Abstract
;
Full Text
Heme oxygenase-1 gene activation by the NAD(P)H oxidase inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride via a protein kinase B, p38-dependent signaling pathway in monocytes:
N. Wijayanti, et al.; J. Biol. Chem.
280
, 21820 (2005)
Abstract
;
Full Text
Inhibitory effect of 4-(2-aminoethyl)-benzenesulfonyl fluoride, a serine protease inhibitor, on PI3K inhibitor-induced CHOP expression:
T. Hosoi, et al.; Eur. J. Pharmacol.
554
, 8 (2007)
Abstract
Further Categories Containing This Product:
Heme Oxygenase / Related Products
•
MAPK Pathway Activators
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Fatty Acid Amide Hydrolase [FAAH] / Related Products
ALX-152-006
Revised 23-Jan-07
Bradykinin
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
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ALX-152-006-M005
5 mg
22.00 USD
ALX-152-006-M025
25 mg
86.00 USD
Product Specification
SEQUENCE:
H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH
FORMULA:
C
50
H
73
N
15
O
11
MW:
1060.3
CAS NUMBER:
5979-11-3
MERCK INDEX:
14:
1359
PURITY:
≥98% (HPLC)
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in 5% acetic acid.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
Product Description
Activator of eNOS (NOS III).
Product Specific Literature References
Different activation of L-arginine pathway by bradykinin, serotonin, and clonidine in coronary arteries:
V. Richard, et al.; Am. J. Physiol.
259
, H1433 (1990)
Abstract
Bradykinin and ATP stimulate L-arginine uptake and nitric oxide release in vascular endothelial cells:
R.G. Bogle, et al.; BBRC
180
, 926 (1991)
Abstract
Activation of a small-conductance Ca(2+)-dependent K+ channel contributes to bradykinin-induced stimulation of nitric oxide synthesis in pig aortic endothelial cells:
K. Groschner, et al.; Biochim. Biophys. Acta
1137
, 162 (1992)
Abstract
G proteins in aortic endothelial cells and bradykinin-induced formation of nitric oxide:
J. Gil-Longo, et al.; Eur. J. Pharmacol.
247
, 119 (1993)
Abstract
Simultaneous measurements of Ca2+ and nitric oxide in bradykinin-stimulated vascular endothelial cells:
L.A. Blatter, et al.; Circ. Res.
76
, 922 (1995)
Abstract
;
Full Text
Biosynthesis of endothelium-derived nitric oxide by bradykinin as endogenous precursor:
A.R. Volpe, et al.; Immunopharmacology
33
, 287 (1996)
Abstract
Nitric oxide as mediator of bradykinin-induced pancreatic circulatory and metabolic responses:
W.W. Pawlik, et al.; J. Physiol. Pharmacol.
48
, 751 (1997)
Abstract
Bradykinin-regulated interactions of the mitogen-activated protein kinase pathway with the endothelial nitric-oxide synthase:
S.G. Bernier, et al.; J. Biol. Chem.
275
, 30707 (2000)
Abstract
;
Full Text
Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation:
M.B. Harris, et al.; J. Biol. Chem.
276
, 16587 (2001)
Abstract
;
Full Text
Bradykinin down-regulates, whereas arginine analogs up-regulates, endothelial nitric-oxide synthase expression in coronary endothelial cells:
N.D. Vaziri, et al.; J. Pharmacol. Exp. Ther.
313
, 121 (2005)
Abstract
;
Full Text
Bradykinin mediates phosphorylation of eNOS in odontoblasts:
Y. Korkmaz, et al.; J. Dent. Res.
85
, 536 (2006)
Abstract
Further Categories Containing This Product:
Peptides
•
NOS Regulation
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
ALX-152-031
Revised 17-Nov-08
(Des-Arg1)-Bradykinin
SYNONYMS
Bradykinin (2-9)
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
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ALX-152-031-M001
1 mg
15.00 USD
ALX-152-031-M005
5 mg
50.00 USD
Product Specification
SEQUENCE:
H-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH
FORMULA:
C
44
H
61
N
11
O
10
MW:
904.0
CAS NUMBER:
16875-11-9
PEPTIDE CONTENT:
≥85%
PURITY:
≥98% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in water (1mg/ml).
QUALITY CONTROL:
Identity determined by MS.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Further Categories Containing This Product:
NOS Regulation
•
Peptides
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
ALX-152-032
Revised 08-Dec-06
(Des-Arg9)-Bradykinin
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
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ALX-152-032-M005
5 mg
75.00 USD
ALX-152-032-M025
25 mg
300.00 USD
Product Specification
SEQUENCE:
H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-OH
FORMULA:
C
44
H
61
N
11
O
10
MW:
904.0
CAS NUMBER:
15958-92-6
PURITY:
≥99%
APPEARANCE:
White lyophilized powder.
SOLUBILITY:
Soluble in water (1mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Further Categories Containing This Product:
Peptides
•
NOS Regulation
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
ALX-430-137
Revised 23-Oct-08
IM-54
SYNONYMS
2-(1
H
-Indol-3-yl)-3-pentylamino-maleimide
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Non-apoptotic Cell Death / Necrosis
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ALX-430-137-M001
1 mg
30.00 USD
ALX-430-137-M025
25 mg
420.00 USD
Product Specification
FORMULA:
C
19
H
23
N
3
O
2
MW:
325.4
PURITY:
≥98% (HPLC)
APPEARANCE:
Yellow powder.
SOLUBILITY:
Soluble in DMSO (100mg/ml), dichloomethane, 100% ethanol or methanol (10mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
Product Description
Cell permeable inhibitor of necrosis. Selectively blocks oxidative stress-induced necrotic cell death (~3µM IM-54 prevented ~50% cell death in HL60 cells exposed to 100µM H
2
O
2
). Does not protect against etoposide-induced apoptosis or display antioxidant properties. Does not affect S6K1 and PKC kinase activities at concentrations of 50µM.
Product Specific Literature References
Inhibition of hydrogen peroxide-induced necrotic cell death with 3-amino-2-indolylmaleimide derivatives:
K. Dodo, et al.; Bioorg. Med. Chem. Lett.
15
, 3114 (2005)
Abstract
Further Categories Containing This Product:
Cell Stress, Oxidative Stress & Redox Signalling Other Products
ALX-460-007
Revised 02-Feb-07
Menadione
SYNONYMS
Vitamin K
3
2-Methyl-1,4-naphthoquinone
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Vitamins
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ALX-460-007-G010
10 g
10.00 USD
Product Specification
FORMULA:
C
11
H
8
O
2
MW:
172.2
CAS NUMBER:
58-27-5
MERCK INDEX:
14:
5831
RTECS:
QL9100000
PURITY:
≥98% (HPLC)
APPEARANCE:
Yellow crystals.
SOLUBILITY:
Soluble in 96% ethanol (16mg/ml) or DMSO (1mg/ml); almost insoluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
HANDLING:
Protect from light.
HAZARD:
HARMFUL.
Product Description
Cytotoxic in various cell lines. Induces apoptosis via elevation of peroxide and superoxide radical levels. Affects gap-junctional intercellular communication by mediation of tyrosine phosphorylation.
Product Specific Literature References
Vitamin K3 induces cell cycle arrest and cell death by inhibiting Cdc25 phosphatase:
F.Y. Wu and T.P.; Eur. J. Cancer
35
, 1388 (1999)
Abstract
Apoptosis-inducing activity of vitamin C and vitamin K:
H. Sakagami, et al.; Cell Mol. Biol.
46
, 129 (2000)
Abstract
Cytotoxic effect through fas/APO-1 expression due to vitamin K in human glioma cells:
L.K. Sun, et al.; J. Neurooncol.
47
, 31 (2000)
Abstract
Cytotoxic activity of vitamins K1, K2 and K3 against human oral tumor cell lines:
H. Okayasu, et al.; Anticancer Res.
21
, 2387 (2001)
Abstract
2-Methyl-1,4-naphthoquinone, vitamin K(3), decreases gap-junctional intercellular communication via activation of the epidermal growth factor receptor/extracellular signal-regulated kinase cascade:
L.O. Klotz, et al.; Cancer Res.
62
, 4922 (2002)
Abstract
;
Full Text
Menadione metabolism to thiodione in hepatoblastoma by scanning electrochemical microscopy:
J. Mauzeroll, et al.; PNAS
101
, 17582 (2004)
Abstract
;
Full Text
Menadione-induced reactive oxygen species generation via redox cycling promotes apoptosis of murine pancreatic acinar cells:
D.N. Criddle, et al.; J. Biol. Chem.
281
, 40485 (2006)
Abstract
;
Full Text
Further Categories Containing This Product:
Antitumor Agents (DNA Interaction & Gene Regulation)
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Antitumor Agents (Apoptosis Inducers)
ALX-804-647
Revised 13-Oct-06
Monoclonal Antibody to Bradykinin (MBK3)
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
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ALX-804-647-R100
100 µl
210.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE:
MBK3
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Undiluted ascites.
IMMUNOGEN:
Bradykinin (H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH).
SPECIFICITY:
Recognizes bradykinin from various species.
APPLICATION:
Western Blot (1:1’000-1:4’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Anti-idiotypic antibodies bearing the internal image of a bradykinin epitope. Production, characterization, and interaction with the kinin receptor:
M. Haasemann, et al.; J. Immunol.
147
, 3882 (1991)
Abstract
Related Products
ALX-152-006
Bradykinin
Further Categories Containing This Product:
NOS Regulation
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Monoclonal Antibodies
ALX-803-302
Revised 06-Aug-07
Monoclonal Antibody to CHP1 (5E9G5)
SYNONYMS
anti-CHORD Containing Protein-1 MAb (5E9G5)
anti-CHORDC1 MAb (5E9G5)
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Cell Stress, Oxidative Stress & Redox Signalling Other Products
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ALX-803-302-C100
100 µg
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
5E9G5
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.2mg/ml
PURITY:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL:
Protein A-affinity purified.
IMMUNOGEN:
Recombinant mouse CHP1 (CHORD containing protein-1).
SPECIFICITY:
Recognizes human and mouse CHP1.
APPLICATION:
ELISA
Immunocytochemistry (5µg/ml)
Immunohistochemistry (5µg/ml)
Immunoprecipitation (5µg per 5x10
6
cells)
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Literature References
Chp-1 and melusin, two CHORD containing proteins in vertebrates:
M. Brancaccio, et al.; FEBS Lett.
551
, 47 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products
•
Nod-complex / Related Products
•
Monoclonal Antibodies
ALX-803-301
Revised 31-Oct-06
Monoclonal Antibody to Melusin (human) (5E1)
SYNONYMS
anti-Integrin β1-binding Protein 2 (human) MAb (5E1)
anti-ITGB1BP2 (human) MAb (5E1)
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Cell Stress, Oxidative Stress & Redox Signalling Other Products
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ALX-803-301-C100
100 µg
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
5E1
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.2mg/ml
PURITY:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL:
Protein A-affinity purified.
IMMUNOGEN:
Recombinant human melusin (integrin β1-binding protein 2).
SPECIFICITY:
Recognizes human melusin. Cross-reacts weakly with mouse melusin.
APPLICATION:
ELISA
Immunocytochemistry (5µg/ml)
Immunohistochemistry (5µg/ml)
Immunoprecipitation
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
GSK-3 Other Products
•
Monoclonal Antibodies
ALX-804-872
Revised 13-Oct-08
Monoclonal Antibody to TIGAR (human) (Woody-1)
SYNONYMS
anti-TP53-induced Glycolysis and Apoptosis Regulator (human) MAb (Woody-1)
PRODUCT LINE
Obesity & Adipokines
PRODUCT CATEGORY
Glycobiology Other Products
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ALX-804-872-C100
100 µg
380.00 USD
Product Specification
CLONE:
Woody-1
ISOTYPE:
Mouse IgG2b
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human TIGAR (aa 1-270).
SPECIFICITY:
Recognizes human TIGAR.
APPLICATION:
Immunoprecipitation (1:200)
Western Blot (1:1‘000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
TIGAR, a p53-inducible regulator of glycolysis and apoptosis:
Bensaad, et al.; Cell
126
, 107 (2006)
Abstract
;
Full Text
Related Products
ALX-210-947
Polyclonal Antibody to TIGAR (AT122)
Further Categories Containing This Product:
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Monoclonal Antibodies
•
Cell Death / Apoptosis / Autophagy Other Products
•
p53 Other Products
ALX-210-872
Revised 11-Nov-05
Polyclonal Antibody to Bradykinin B2 Receptor
SYNONYMS
anti-B
2
R PAb
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
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ALX-210-872-R200
200 µl
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Undiluted serum containing 0.01% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 356-391 of human B
2
R (bradykinin B
2
receptor).
SPECIFICITY:
Recognizes human, mouse and rat B
2
R.
APPLICATION:
Immunoprecipitation (use 2-5µl of undiluted antiserum per precipitate)
Western Blot (1:100-1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Ligand-induced phosphorylation/dephosphorylation of the endogenous bradykinin B2 receptor from human fibroblasts
:
A. Blaukat, et al.; J. Biol. Chem.
271
, 32366 (1996)
Abstract
;
Full Text
Overexpression and functional characterization of kinin receptors reveal subtype-specific phosphorylation
:
A. Blaukat, et al.; Biochemistry
38
, 1300 (1999)
Abstract
Bradykinin-induced internalization of the human B2 receptor requires phosphorylation of three serine and two threonine residues at its carboxyl tail
:
A. Pizard, et al.; J. Biol. Chem.
274
, 13738 (1999)
Abstract
;
Full Text
Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function
:
A. Blaukat, et al.; J. Biol. Chem.
276
, 40431 (2001)
Abstract
;
Full Text
Functional specialization of calreticulin domains
:
K. Nakamura, et al.; J. Cell Biol.
154
, 961 (2001)
Abstract
Further Categories Containing This Product:
NOS Regulation
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Polyclonal Antibodies
ALX-210-205
Revised 15-May-07
Polyclonal Antibody to Peroxisomal Membrane Protein 70 (mouse)
SYNONYMS
anti-PMP70 (mouse) PAb
anti-ABCD3 (mouse) PAb
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Cell Stress, Oxidative Stress & Redox Signalling Other Products
