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Respiratory Chain / Related Products
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AG-N0812 Revised 21-Nov-08 New product
Nampt/Visfatin (mouse/rat) (IntraCellular) Dual ELISA Kit
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse/rat) (IntraCellular) Dual ELISA Kit
PBEF1 (mouse/rat) (IntraCellular) Dual ELISA Kit
Pre-B-Cell Colony Enhancing Factor 1 (mouse/rat) (IntraCellular) Dual ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-N0812EK-KI01   96 wells 640.00 USD Add To Cart
AG-N0812TP-KI01 Twin Plex 2x96 wells 940.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SENSITIVITY: 50pg/ml (range 0 to 64ng/ml)
SPECIFICITY: Does not cross-react with human Nampt/visfatin.
APPLICATION: For the quantitative determination of Nampt/visfatin in mouse or rat cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
NAD+ Metabolism / Related Products • ELISA & EIA Kits
 
 
AG-N0823 Revised 23-Oct-08 New product
Nampt/Visfatin (human) (IntraCellular) ELISA Kit
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PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-N0823EK-KI01   96 wells 640.00 USD Add To Cart
AG-N0823TP-KI01 Twin Plex 2x96 wells 940.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 30pg/ml (range 0 to 32ng/ml)
SPECIFICITY: Does not cross-react with mouse sera.
APPLICATION: For the quantitative determination of Nampt/Visfatin in human cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
NAD+ Metabolism / Related Products • ELISA & EIA Kits
 
 
AG-V0523 Revised 01-Dec-08
Nampt/Visfatin (human) ELISA Kit
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SYNONYMS Nicotinamide Phosphoribosyltransferase (human) ELISA Kit
PBEF1 (human) ELISA Kit
Pre-B-Cell Colony Enhancing Factor 1 (human) ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-V0523EK-KI01   96 wells 660.00 USD Add To Cart
AG-V0523TP-KI01 Twin Plex 2x96 wells 920.00 USD Add To Cart
AG-V0523PP-KI01 Penta Plex 5x96 wells 2'050.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 30pg/ml (range 0 to 16ng/ml)
SPECIFICITY: Does not cross-react with mouse Nampt/visfatin. Has been shown to specifically detect the physiologically relevant Nampt/visfatin dimer in human serum samples (see [2]).
APPLICATION: For the quantitative determination of human Nampt/visfatin in serum.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway: T. Yonezawa, et al.; FEBS Lett. 580, 6635 (2006) Abstract
[2] Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007) Abstract; Full Text
[3] Correlation of circulating full-length visfatin (PBEF/Nampt) with metabolic parameters in subjects with and without diabetes: a cross-sectional study: R. Retnakaran, et al.; Clin. Endocrinol. (Oxf) 96, 885 (2008) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
NAD+ Metabolism / Related Products • ELISA & EIA Kits
 
 
AG-VN0812 Revised 21-Nov-08 New product
Nampt/Visfatin (mouse/rat) Dual ELISA Kit
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse/rat) Dual ELISA Kit
PBEF1 (mouse/rat) Dual ELISA Kit
Pre-B-Cell Colony Enhancing Factor 1 (mouse/rat) Dual ELISA Kit
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-VN0812EK-KI01   96 wells 660.00 USD Add To Cart
AG-VN0812TP-KI01 Twin Plex 2x96 wells 920.00 USD Add To Cart
AG-VN0812PP-KI01 Penta Plex 5x96 wells 2'050.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SENSITIVITY: 50pg/ml (range 0 to 64ng/ml)
SPECIFICITY: Does not cross-react with human Nampt/visfatin.
APPLICATION: For the quantitative determination of Nampt/visfatin in mouse or rat serum.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
NAD+ Metabolism / Related Products • ELISA & EIA Kits
 
 
ALX-201-235 Revised 17-Aug-06
NAD Kinase (full length) (active) (human) (recombinant) (His)
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SYNONYMS NADK (full length) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (full length) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-235-C050   50 µg 320.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK is fused at the N-terminus to a His-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 weeks when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Enzymes • Kinases Other Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-236 Revised 17-Aug-06
NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
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SYNONYMS NADK (NT truncated) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-236-C050   50 µg 260.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK (aa 64-446) is fused at the N-terminus to a His-tag.
QUANTITY: Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Liquid. Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SPECIFIC ACTIVITY: ≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Enzymes • Kinases Other Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-237 Revised 12-Aug-08
NMNAT1 (human) (recombinant) (His)
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SYNONYMS NMN Adenylyltransferase 1 (human) (recombinant) (His)
Nicotinamide Mononucleotide Adenylyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
Ordering Information
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ALX-201-237-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 33.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT1 is fused at the N-terminus to a His-tag.
CONCENTRATION:

~1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
SPECIFIC ACTIVITY: 10-20U/mg
APPLICATION: Well suited for the synthesis of NAD due to high specific activity (10-20U/mg) and high substrate selectivity compared to NMNAT3 (human) (recombinant) (Prod. No. ALX-201-238).
Note: When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min. before the assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C. Unstable in solutions containing less than 250mM salt.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem. 276, 406 (2001) Abstract; Full Text
Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett. 492, 95 (2001) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9HAN9: NMNAT1 (human)
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Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-238 Revised 18-Mar-08
NMNAT3 (human) (recombinant)
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SYNONYMS NMN Adenylyltransferase 3 (human) (recombinant)
Nicotinamide Mononucleotide Adenylyltransferase 3 (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-238-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 30.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT3 is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.8mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
APPLICATION: Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1 (human) (recombinant) (Prod. No. ALX-201-237).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis: X. Zhang, et al.; J. Biol. Chem. 278, 13503 (2003) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96T66: NMNAT3 (human)
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Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-318 Revised 23-Oct-08
Nampt/Visfatin (mouse) (recombinant) (His)
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SYNONYMS Nicotinamide Phosphoribosyltransferase (mouse) (recombinant) (His)
PBEF1 (mouse) (recombinant) (His)
Pre-B-Cell Colony Enhancing Factor 1 (mouse) (recombinant) (His)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Visfatin / Nampt / PBEF / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-318-C050   50 µg 395.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Full length mouse Nampt/visfatin (aa 1-491) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
APPEARANCE: Lyophilized. Salt-free.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL test)
RECONSTITUTION: Reconstitute with an appropriate buffer.
BIOLOGICAL ACTIVITY: Shows adipogenic effects in differentiating 3T3-L1 cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol. 178, 1748 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot links Q9JKM0 & Q99KQ4: Pre-B-Cell Colony Enhancing Factor 1 (mouse)
MANUFACTURER Manufactured by AdipoGen, Inc.
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