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Items 18 of 18
ALX-210-069
Revised 16-Jul-08
Polyclonal Antibody to HIF-1α
SYNONYMS
anti-Hypoxia-inducible Factor 1α PAb
anti-ARNT-interacting Protein PAb
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
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ALX-210-069-C050
50 µg
220.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
2.8mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN:
Recombinant HIF-1α (hypoxia-inducible factor-1a) (aa 609-826).
SPECIFICITY:
Recognizes human and mouse HIF-1α.
APPLICATION:
Immunohistochemistry (1:200)
Immunoprecipitation (1-5µg)
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Loss of HIF-1alpha in endothelial cells disrupts a hypoxia-driven VEGF autocrine loop necessary for tumorigenesis:
N. Tang, et al.; Cancer Cell
6
, 485 (2004)
Abstract
Epidermal sensing of oxygen is essential for systemic hypoxic response:
A.T. Boutin, et al.; Cell
133
, 206 (2008)
Abstract
Critical Role of HIF-1alpha in Keratinocyte Defense against Bacterial Infection:
C. Peyssonnaux, et al.; J. Invest. Dermatol.
128
, 1964 (2008)
Abstract
General Literature References
Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin
:
C. C. Hudson, et al.; Mol. Cell Biol.
22
, 7004 (2002)
Abstract
;
Full Text
Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products
•
Polyclonal Antibodies
ALX-210-150
Revised 15-Oct-07
Polyclonal Antibody to Ah Receptor Nuclear Translocator (mouse)
SYNONYMS
anti-ARNT (mouse) PAb
anti-HIF-1β (mouse) PAb
anti-Hypoxia-inducible Factor 1β (mouse) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Aryl Hydrocarbon Receptor / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-210-150-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 771-789 (N
771
SYNNEEFPDLTMFPPFSE
789
) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY:
Recognizes mouse ARNT.
APPLICATION:
Western Blot (1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
General Literature References
Cloning of a factor required for activity of the Ah (Dioxin) receptor
:
E. Hoffman, et al.; Science
252
, 954 (1991)
Abstract
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocation: characterization of two monoclonal antibodies to the ARNT
:
N.G. Hord and G. Perdew; Mol. Pharmacol.
46
, 618 (1994)
Abstract
cDNA cloning and tissue-specific expression of a novel basic helix/loop/helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear transporter
:
K. Hirose, et al.; Mol. Cell. Biol.
16
, 1706 (196)
Abstract
Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts
:
C. Vaziri, et al.; J. Biol. Chem.
271
, 25921 (1996)
Abstract
;
Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products
•
Polyclonal Antibodies
ALX-215-058
Revised 12-Sep-07
Polyclonal Antibody to HIF-2α (human)
SYNONYMS
anti-Hypoxia-inducible Factor 2α (human) PAb
anti-EPAS-1 (human) PAb
anti-Endothelial PAS Domain-containing Protein 1 (human) PAb
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
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ALX-215-058-R200
200 µl
267.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 426-443 of human HIF-2α (hypoxia-inducible factor 2α).
SPECIFICITY:
Recognizes human HIF-2α. Detects a band of ~90kDa.
APPLICATION:
Western Blot (1:500-1:1'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 6 months when stored at -4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Images
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Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products
•
Polyclonal Antibodies
ALX-270-371
Revised 18-Jun-07
Dimethyloxalyl glycine
SYNONYMS
DMOG
N-(Methoxyoxoacetyl)-glycine methyl ester
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-270-371-M010
10 mg
18.00 USD
ALX-270-371-M050
50 mg
72.00 USD
ALX-270-371-M100
100 mg
120.00 USD
Product Specification
FORMULA:
C
6
H
9
NO
5
MW:
175.1
CAS NUMBER:
89464-63-1
PURITY:
≥98%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in 100% ethanol, DMSO or dimethyl formamide; also soluble in PBS.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1
year when stored at -20°C. Stock solutions are stable for at least 6 months if stored at -20°C.
Product Description
Cell permeable, competitive inhibitor of PHDs (prolyl hydroxylase domain-containing proteins; HIF-PHs), which destructs HIF-1α when hydroxylated at a specific proline residue (P
564
). Stabilizes HIF-1α expression at normal oxygen tensions in cultured cells at concentrations between 0.1 and 1mM. Expected to act pro-angiogenic.
Product Specific Literature References
Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
:
P. Jaakkola, et al.; Science
292
, 468 (2001)
Abstract
A conserved family of prolyl-4-hydroxylases that modify HIF
:
R.K. Bruick & S.L. McKnight; Science
294
, 1337 (2001)
Abstract
HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing
:
M. Ivan, et al.; Science
292
, 464 (2001)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
A stock solution may be made by dissolving the DMOG in an organic solvent purged with an inert gas. DMOG is soluble in organic solvents such as ethanol, DMSO and dimethyl formamide.The solubility of DMOG in these solvents is at least 30mg/ml. DMOG will be stable for at least six months in these solvents if stored at -20°C.
Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of DMOG can be prepared by directly dissolving the crystalline compound powder in aqueous buffers. The solubility of DMOG in PBS (pH 7.2) is at least 10mg/ml.
ALX-270-372
Revised 08-Jul-08
2,4-Diethylpyridine dicarboxylate
SYNONYMS
2,4-DPD
2,4-Pyridinedicarboxylic acid diethyl ester
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-270-372-M010
10 mg
23.00 USD
ALX-270-372-M025
25 mg
53.00 USD
ALX-270-372-M100
100 mg
177.00 USD
Product Specification
FORMULA:
C
11
H
13
NO
4
MW:
223.2
CAS NUMBER:
41438-38-4
CONCENTRATION:
50mg/ml
PURITY:
≥98%
FORMULATION:
Liquid. Solution in ethanol.
SOLUBILITY:
Soluble in 100% ethanol (50mg/ml), DMSO (20mg/ml) or dimethyl formamide (30mg/ml); slightly soluble in PBS, pH 7.2 (1mg/ml).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at –20°C. We do not recommend storing aqueous solutions for more than one day.
Product Description
2,4-DPD is a cell permeable, competitive inhibitor of the oxygen-sensing enzyme HIF prolyl hydroxylase (HIF-PH), which destructs HIF-1α when hydroxylated at a specific proline residue (P564). 2,4-DPD inhibits the hydroxylation of P564 by acting as a competitive inhibitor of the HIF-PH cofactor α-keto glutarate, with effective concentrations in the low µM range. 2,4-DPD is therefore expected to act as a pro-angiogenic compound, acting via the HIF-1α system.
Product Specific Literature References
Prolyl 4-hydroxylase is required for viability and morphogenesis in Caenorhabditis elegans
:
L. Friedman, et al.; PNAS
97
, 4736 (2000)
Abstract
;
Full Text
HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing
:
M. Ivan, et al.; Science
292
, 464 (2001)
Abstract
Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
:
P. Jaakkola, et al.; Science
292
, 468 (2001)
Abstract
A conserved family of prolyl-4-hydroxylases that modify HIF
:
R.K. Bruick & S.L. McKnight; Science
294
, 1337 (2001)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
To change the solvent, simply evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
ALX-270-412
Revised 23-Mar-06
N-Oxalylglycine
SYNONYMS
NOG
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-270-412-M010
10 mg
40.00 USD
ALX-270-412-M050
50 mg
120.00 USD
Product Specification
FORMULA:
C
4
H
5
NO
5
MW:
147.1
CAS NUMBER:
5262-39-5
PURITY:
≥98%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in water or DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HAZARD:
IRRITANT.
Product Description
Cell permeable, competitive inhibitor of PHDs (prolyl hydroxylase domain-containing proteins; HIF-PHs).
Product Specific Literature References
Novel inhibitors of prolyl 4-hydroxylase. 3. Inhibition by the substrate analogue N-oxaloglycine and its derivatives
:
C.J. Cunliffe, et al.; J. Med. Chem.
35
, 2652 (1992)
Abstract
General Literature References
Inhibition of prolyl 4-hydroxylase by oxalyl amino acid derivatives in vitro, in isolated microsomes and in embryonic chicken tissues
:
E. Baader, et al.; Biochem. J.
300
, 525 (1994)
Abstract
Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
:
P. Jaakkola, et al.; Science
292
, 468 (2001)
Abstract
C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation
:
A.C. Epstein, et al.; Cell
107
, 43 (2001)
Abstract
Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha
:
J.M. Elkins, et al.; J. Biol. Chem.
278
, 1802 (2003)
Abstract
Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases
:
P. Koivunen, et al.; J. Biol. Chem.
279
, 9899 (2004)
Abstract
ALX-270-413
Revised 23-Mar-06
N-Oxalyl-L-alanine
SYNONYMS
NOLA
N-Oxalyl-2S-alanine
NOxSA
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
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ALX-270-413-M010
10 mg
60.00 USD
ALX-270-413-M050
50 mg
180.00 USD
Product Specification
FORMULA:
C
5
H
7
NO
5
. H
2
O
MW:
161.1 . 18.0
PURITY:
≥98% (TLC)
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in water or DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Very hygroscopic.
Product Description
Cell permeable, competitive inhibitor of PHDs (prolyl hydroxylase domain-containing proteins; HIF-PHs). Less potent inhibitor of factor inhibiting HIF asparagine hydroxylase (FIH, IC
50
=2.5mM).
Product Specific Literature References
Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
:
P. Jaakkola, et al.; Science
292
, 468 (2001)
Abstract
Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha
:
J.M. Elkins, et al.; J. Biol. Chem.
278
, 1802 (2003)
Abstract
ALX-270-414
Revised 06-Jul-06
N-Oxalyl-D-alanine
SYNONYMS
NODA
N-Oxalyl-2R-alanine
NOxRA
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Hypoxia-inducible Factor [HIF] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-270-414-M010
10 mg
80.00 USD
ALX-270-414-M050
50 mg
240.00 USD
Product Specification
FORMULA:
C
5
H
7
NO
5
. H
2
O
MW:
161.1 . 18.0
PURITY:
≥98%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in water or DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Very hygroscopic.
Product Description
Cell permeable, potent competitive inhibitor of factor inhibiting HIF asparagine hydroxylase (FIH, IC
50
<0.4mM). Does not inhibit PHDs (prolyl hydroxylase domain-containing proteins; HIF-PHs).
Product Specific Literature References
Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation
:
P. Jaakkola, et al.; Science
292
, 468 (2001)
Abstract
Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha
:
J.M. Elkins, et al.; J. Biol. Chem.
278
, 1802 (2003)
Abstract
ALX-350-092
Revised 03-Apr-08
Emetine . dihydrochloride
SYNONYMS
6’,7’,10,11-Tetramethoxyemetan . 2HCl
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - DNA Replication Inhibitors
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ALX-350-092-M050
50 mg
10.00 USD
Product Specification
FORMULA:
C
29
H
40
N
2
O
4
. 2HCl
MW:
480.7 . 73.0
CAS NUMBER:
316-42-7
MERCK INDEX:
14:
3559
SOURCE/HOST:
Isolated from ground roots of
Uragoga ipecacuanha
.
PURITY:
≥98%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in water or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light. Hygroscopic.
HAZARD:
TOXIC.
Product Description
Irreversibly blocks protein synthesis by inhibiting the movement of ribosome along the mRNA. Induces hypotension by blocking adrenoreceptors. Inhibits DNA replication in the early S phase. Inhibits HIF-1 activation by hypoxia. Induces apoptosis in leukemia cells. Antiamebic.
Product Specific Literature References
Inhibitors of protein biosynthesis. V. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells:
A.P. Grollman; J. Biol. Chem.
243
, 4089 (1968)
Abstract
Cytometric analysis of DNA replication inhibited by emetine and cyclosporin A::
A: T. Schweighoffer, et al.; Histochemistry
96
, 93 (1991)
Abstract
Emetine allows identification of origins of mammalian DNA replication by imbalanced DNA synthesis, not through conservative nucleosome segregation:
W.C. Burhans, et al.; EMBO J.
10
, 4351 (1991)
Abstract
Role of newly synthesized MHC class II molecules in antigen-specific antigen presentation by B cells:
T. Kokuho, et al.; Immunobiology
193
, 42 (1995)
Abstract
Terpenoid tetrahydroisoquinoline alkaloids emetine, klugine, and isocephaeline inhibit the activation of hypoxia-inducible factor-1 in breast tumor cells:
Y.D. Zhou, et al.; J. Nat. Prod.
68
, 947 (2005)
Abstract
The alkaloid emetine as a promising agent for the induction and enhancement of drug-induced apoptosis in leukemia cells:
M. Moller, et al.; Oncol. Rep.
18
, 737 (2007)
Abstract
Characteristics of apoptosis induction by the alkaloid emetine in human tumour cell lines:
M. Moller & M. Wink; Planta Med.
73
, 1389 (2007)
Abstract
Further Categories Containing This Product:
Other Toxins
•
Adrenergics & Adrenergic Receptors / Related Products
•
Translation Inhibitors
•
Hypoxia-inducible Factor [HIF] / Related Products
•
Natural Products - Other Anti-infective Agents
•
Parasitic Diseases Other Products
•
Natural Products for Neurological Research
•
DNA Replication Inhibitors
•
Natural Products - Apoptosis Inducers & Inhibitors
•
Alkaloids
ALX-350-128
Revised 03-Apr-08
Chetomin
SYNONYMS
Chaetomin
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Antibiotics - Immunomodulators
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ALX-350-128-M001
1 mg
185.00 USD
