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Stress & Heat Shock Proteins
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ALX-804-074 Revised 09-Jul-08
Monoclonal Antibody to HSP70 (universal) (5A5)
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SYNONYMS anti-Heat Shock Protein 70 (universal) MAb (5A5)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-074-R250   250 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE: 5A5
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human HSP70 (heat shock protein 70).
SPECIFICITY: Recognizes several members of the HSP70 gene family, including HSP70, HSC70, GRP78, and following heat shock, HSP72 in a broad range of species, including human, mouse, avian, frog, fish, drosophila, and yeast HSP70. Detects bands from ~70 to ~78kDa by Western blot, representing different members of the HSP70 family. 2-D-gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts.
CROSSREACTIVITY PROTEINS: HSP70, HSC70, GRP78, HSP72 (following heat shock)
APPLICATION: Gel Shift Assay
Immunocytochemistry (1:100)
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-077 Revised 15-Jun-06
Monoclonal Antibody to mtHSP70 (JG1)
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SYNONYMS anti-Heat Shock Protein 70 (mitochondrial) MAb (JG1)
anti-GRP75 MAb (JG1)
anti-Mortalin MAb (JG1)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Mitochondrial Heat Shock Proteins [mHSPs] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-077-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
Monkey
CLONE: JG1
ISOTYPE: Mouse IgG3
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to C-terminal aa 615-633 (G615SGSSGTGEQKEDQKEEKQ633) of mouse mtHSP70 (mitochondrial heat shock protein 70).
SPECIFICITY: Recognizes human, mouse, dog and monkey mtHSP70. Detects a band of ~75kDa by Western blot.
APPLICATION: Immunocytochemistry (1:50)
Immunoprecipitation
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Generation and characterization of monoclonal antibodies specific for members of the mammalian 70-kDa heat shock protein family: J.M. Green, et al.; Hybridoma 14, 347 (1995) Abstract
Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1 or SURF1: S.L. Williams, et al.; J. Biol. Chem. 279, 7462 (2004) Abstract
The life span determinant p66Shc localizes to mitochondria where it associates with mitochondrial heat shock protein 70 and regulates trans-membrane potential: F. Orsini, et al.; J. Biol. Chem. 279, 25689 (2004) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-241 Revised 30-May-08
Monoclonal Antibody to JNK/p38 (phosphorylated) (9H8)
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SYNONYMS anti-SAPK1/2 (phosphorylated) MAb (9H8)
anti-SAP Kinase 1/2 (phosphorylated) MAb (9H8)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY JNK [SAPK1] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-241-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9H8
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of phosphorylated JNK and p38.
SPECIFICITY: Recognizes the TG/PpT motif of activated, monophosphorylated and the dually phosphorylated site pTG/PpT of human and mouse JNK and p38. Does not cross-react with the non-phosphorylated form of the protein or with activated ERK1 or ERK2.
APPLICATION: Immunohistochemistry
Immunoprecipitation
Western Blot: Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL: Included (Prod. No. ALX-840-037).
Product Description
Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines
Further Categories Containing This Product:
Phospho-specific Antibodies • p38 Kinases / Related Products • Monoclonal Antibodies
 
 
ALX-804-285 Revised 13-Jul-06
Monoclonal Antibody to Mcl-1 (human) (13)
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SYNONYMS anti-Myeloid Cell Leukemia-1 (human) MAb (13)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Mcl-1 / Related Products
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ALX-804-285-L010   10 ml 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 13
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Tissue culture supernatant containing 0.05% sodium azide.
IMMUNOGEN: Recombinant peptide from rat Mcl-1 (myeloid cell leukemia-1).
SPECIFICITY: Recognizes human Mcl-1. Detects a doublet of ~40-42kDa by Western blot.
APPLICATION: Western Blot (1:10)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Formation of a fine precipitate may occur.
HANDLING: Avoid freeze/thaw cycles.
Product Description
 
Product Specific Literature References
[1] MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2: K.M. Kozopas, et al.; PNAS 90, 3516 (1993) Abstract; Full Text
[2] Regulation of MCL1 through a serum response factor/Elk-1-mediated mechanism links expression of a viability-promoting member of the BCL2 family to the induction of hematopoietic cell differentiation: K.J. Townsend, et al.; J. Biol. Chem. 274, 1801 (1999) Abstract; Full Text
[3] Exon skipping in Mcl-1 results in a bcl-2 homology domain 3 only gene product that promotes cell death: C.D. Bingle, et al.; J. Biol. Chem. 275, 22136 (2000) Abstract; Full Text
[4] MCL-1S, a splicing variant of the antiapoptotic BCL-2 family member MCL-1, encodes a proapoptotic protein possessing only the BH3 domain: J. Bae, et al.; J. Biol. Chem. 275, 25255 (2000) Abstract; Full Text
General Information
Mcl-1 (myeloid cell leukemia-1) was first discovered as an early induction gene during the differentiation of a human myeloid leukemia cell line [1] and belongs to the Bcl-2 family. Whereas full-length Mcl-1 possesses BH1-, BH2- and BH3-domains and acts anti-apoptotic, a splice variant exists that through exon skipping only possesses the BH3-domain and so becomes pro-apoptotic [2,3].
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-852 Revised 28-Apr-08
Monoclonal Antibody to Omentin 1 (Saly-2)
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SYNONYMS anti-Intelectin-1 MAb (Saly-2)
anti-Intestinal Lactoferrin Receptor MAb (Saly-2)
anti-Galactofuranose-binding Lectin MAb (Saly-2)
anti-Endothelial Lectin HL-1 MAb (Saly-2)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Omentin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-852-C100   100 µg 360.00 USD Add To Cart
ALX-804-852B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: Saly-2
ISOTYPE: Mouse IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human omentin 1 (intelectin-1) (aa 17-231).
SPECIFICITY: Recognizes human omentin 1 and rat omentin. Cross-reacts with human omentin 2.
APPLICATION: ELISA (detection)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
General Information
Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been recently identified as a novel visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38 kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab. 290, E1253 (2006) Abstract
Related Products
Further Categories Containing This Product:
PI(3)K-Akt-mTOR Pathway Other Products • Monoclonal Antibodies
 
 
ALX-804-850 Revised 16-Aug-07
Monoclonal Antibody to Omentin 1 (human) (Saly-1)
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SYNONYMS anti-Intelectin-1 (human) MAb (Saly-1)
anti-Intestinal Lactoferrin Receptor (human) MAb (Saly-1)
anti-Galactofuranose-binding Lectin (human) MAb (Saly-1)
anti-Endothelial Lectin HL-1 (human) MAb (Saly-1)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Omentin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-850-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Saly-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human omentin 1 (intelectin-1) (aa 17-231).
SPECIFICITY: Recognizes human omentin 1.
APPLICATION: ELISA (capture)
Immunohistochemistry (paraffin sections)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
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General Information
Omentin 1 (also named intelectin-1, endothelial lectin HL-1 and intestinal lactoferrin receptor) has been recently identified as a novel visceral fat (or omental fat) depot-specific adipokine. Omentin 1 is a secreted protein of ~38 kDa containing a fibrinogen-related domain. Omentin 1 is highly expressed in the omental fat, and much less in intestine, lung and heart. Addition of recombinant omentin in vitro did not affect basal, but enhanced insulin-stimulated glucose uptake in subcutaneous as well as in omental human adipocytes. Omentin 1 triggers AKT signalling in the absence of insulin.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action: R.Z. Yang, et al.; Am. J. Physiol. Endocrinol. Metab. 290, E1253 (2006) Abstract
Related Products
Further Categories Containing This Product:
PI(3)K-Akt-mTOR Pathway Other Products • Monoclonal Antibodies
 
 
ALX-804-186 Revised 04-Sep-07
Monoclonal Antibody to p53 (phosphorylated) (pSer392) (9F4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-186-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1 ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of p53 phosphorylated at Ser392.
SPECIFICITY: Recognizes activated human p53 phosphorylated at Ser392. Does not cross-react with the non-phosphorylated form of p53 or with unrelated serine-phosphorylated proteins.
APPLICATION: Western Blot: 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies • Tumor Markers
 
 
ALX-801-060 Revised 20-May-08
Monoclonal Antibody to p53 (human) (BP53-12)
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SYNONYMS anti-p53 (human) MAb (BP53-12)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-060-C100   100 µg 210.00 USD Add To Cart
ALX-801-060F-C100 FITC 100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: BP53-12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human wild-type p53 protein.
SPECIFICITY: Antibody BP53-12 detects a denaturation resistant epitope (aa 16-25) in the human p53 protein.
APPLICATION: BP53-12:
ELISA
Immunocytochemistry
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1-2µg/ml)
BP53-12-FITC:
Flow Cytometry
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies: J. Bartek, et al.; J. Pathol. 169, 27 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-013 Revised 29-Sep-05
Monoclonal Antibody to PDI (RL77)
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SYNONYMS anti-Protein Disulfide-isomerase MAb (RL77)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Synthesis, Modification & Degradation Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-013-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: RL77
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Ascites fluid containing 0.05% sodium azide.
IMMUNOGEN: Purified rat liver PDI (protein disulphide-isomerase).
SPECIFICITY: Recognizes human, mouse, rat and hamster and Xenopus laevis PDI. Detects a band of ~59kDa (rat liver PDI) or ~61KDa (human liver PDI).
APPLICATION: Immunohistochemistry (paraffin sections (1:100))
Immunoprecipitation (inhibits activity of PDI in vitro)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies: C.S. Kaetzel, et al.; Biochem. J. 241, 39 (1987) Abstract
General Information
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Monoclonal Antibodies
 
 
ALX-804-012 Revised 09-Jun-08
Monoclonal Antibody to PDI (RL90)
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SYNONYMS anti-Protein Disulfide-isomerase MAb (RL90)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY