ALEXIS Biochemicals: Monoclonal Antibody to BAP31 (CC-1)

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ALX-201-269

Revised 18-Mar-08

Calreticulin (rabbit) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calreticulin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-269-C025		25 µg	170.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	1.2mg/ml
PURITY:	≥99%
FORMULATION:	Liquid. In 100mM HEPES, pH 7.5, containing 150mM NaCl and 5mM CaCl₂.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Delineation of the lectin site of the molecular chaperone calreticulin: S.P. Thomson and D.B. Williams; Cell Stress Chaperones 10, 242 (2005) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P15253: Calreticulin (rabbit)

Further Categories Containing This Product:

ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Recombinant Proteins / Fusion Proteins • Endoplasmatic Reticulum Stress

ALX-210-126

Revised 07-Aug-07

Polyclonal Antibody to Calreticulin
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calreticulin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-126-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Others
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Antiserum containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human calreticulin.
SPECIFICITY:	Recognizes human, mouse, rat, rabbit and monkey calreticulin.
APPLICATION:	Immunohistochemistry (1:100) Immunocytochemistry Immunoprecipitation Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils: O. Stendahl, et al.; Science 265, 1439 (1994) Abstract
Cell surface calreticulin is a putative mannoside lectin which triggers mouse melanoma cell spreading: T.K. White, et al.; J. Biol. Chem. 270, 15926 (1995) Abstract; Full Text
Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin: A. Otteken & B. Moss; J. Biol. Chem. 271, 97 (1996) Abstract; Full Text

Further Categories Containing This Product:

ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Endoplasmatic Reticulum Stress • Polyclonal Antibodies

ALX-210-141

Revised 10-Jan-05

Polyclonal Antibody to KDEL/GRP78
PRODUCT LINE	Stress & Heat Shock Proteins
PRODUCT CATEGORY	Stress & Heat Shock Proteins Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-141-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Mouse Rat Others
SOURCE/HOST:	From rabbit.
FORMULATION:	Lyophilized. Purified IgG containing 0.05% sodium azide.
RECONSTITUTION:	Reconstitute in PBS.
IMMUNOGEN:	Synthetic peptide corresponding to aa 643-654 (CT⁶⁴³GEEDTSEKDEL⁶⁵⁴) of rat GRP78 (glucose regulated protein 78).
SPECIFICITY:	Recognizes mouse, rat and hamster endoplasmic reticulum (ER) targeting sequence KDEL. Does not cross-react with human endoplasmic reticulum (ER) targeting sequence KDEL.
APPLICATION:	Immunocytochemistry Immunoprecipitation Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Polyclonal Antibodies

ALX-210-170/1

Revised 19-May-08

Polyclonal Antibody to Calreticulin (NT)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Calreticulin / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-170/1-R100		100 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog Others
SOURCE/HOST:	From chicken.
CONCENTRATION:	1.1mg/ml
PURITY DETAIL:	Epitope-affinity purified IgY.
FORMULATION:	Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 24-43 (K²⁴EQFLDGDAWTNRWVESKHK⁴³) of mouse calreticulin.
SPECIFICITY:	Recognizes human, mouse, rat, dog and hamster calreticulin. Detects a band of ~60-66kDa by Western blot.
APPLICATION:	Immunocytochemistry Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
BLOCKING PEPTIDE:	For Blocking Peptide See Prod. No. ALX-153-017.

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Related Products

ALX-153-016

Blocking Peptide for PAb to Calreticulin (CT) (Prod. No. ALX-210-171)

Further Categories Containing This Product:

Endoplasmatic Reticulum Stress • ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Polyclonal Antibodies

ALX-804-601

Revised 20-Jun-08

Monoclonal Antibody to BAP31 (A1/182)
SYNONYMS	anti-BCR-associated Protein BAP31 MAb (A1/182) anti-p28 BAP31 MAb (A1/182) anti-CDM MAb (A1/182) anti-DXS1357E MAb (A1/182) anti-6C6-AG Tumor-associated Antigen MAb (A1/182)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	B Cell Receptor [BCR] & Co-Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-601-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Monkey
CLONE:	A1/182
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml after reconstitution.
FORMULATION:	Lyophilized. Protein G-affinity purified and gel filtrated (FPLC).
RECONSTITUTION:	Reconstitute with 100µl PBS containing 0.1% sodium azide.
IMMUNOGEN:	BAP31 (BCR associated protein BAP031) from Vero monkey cells.
SPECIFICITY:	Recognizes human and monkey BAP31.
APPLICATION:	ELISA Flow Cytometry Immunocytochemistry (1:1’000) Immunohistochemistry (frozen sections) Immunoprecipitation (2µl) Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci. 111, 3411 (1998) Abstract; Full Text
A novel direct interaction of endoplasmic reticulum with microtubules: D.R. Klopfenstein, et al.; EMBO J. 17, 6168 (1998) Abstract; Full Text
Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment: D.R. Klopfenstein, et al.; J. Cell Biol. 153, 1287 (2001) Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004) Abstract; Full Text
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005) Abstract; Full Text
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008) Abstract

General Information

BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca²⁺-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.

General Literature References

Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000) Abstract; Full Text
Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol. 160, 1115 (2003) Abstract; Full Text
Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell 19, 1825 (2008) Abstract; Full Text

Further Categories Containing This Product:

Endoplasmatic Reticulum Stress • ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products

ALX-804-602

Revised 19-Jun-08

Monoclonal Antibody to ERGIC-53 (G1/93)
SYNONYMS	anti-ER-Golgi Intermediate Compartment 53kDa Protein MAb (G1/93) anti-LMAN1 MAb (G1/93)
PRODUCT LINE	Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY	ER-Golgi Intermediate Compartment [ERGIC] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-602-C100		100 µg	346.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Pig Monkey
CLONE:	G1/93
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95%
PURITY DETAIL:	Affinity purified (HYDRA column) and gel filtrated (FPLC).
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with 100µl PBS containing 0.1% sodium azide.
IMMUNOGEN:	Human ERGIC-53 (ER-golgi intermediate compartment 53kDa protein) from Caco-2 cell golgi membrane fraction.
SPECIFICITY:	Recognizes human ERGIC-53. Cross-reacts with pig and monkey ERGIC-53.
APPLICATION:	ELISA Flow Cytometry Immunocytochemistry (1:1’000) Immunohistochemistry (frozen sections) Immunoprecipitation (2µl) Western Blot (1:250)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Description

The ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 is a mannose-specific membrane lectin operating as a cargo receptor for the transport of glycoproteins from the ER to the ERGIC. ERGIC-53 can also be used for studying aspects of protein trafficking in the secretory pathway.

Product Specific Literature References

Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus: A. Schweizer, et al.; J. Cell Biol. 107, 1643 (1988) Abstract; Full Text
The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi: A. Schweizer, et al.; J. Cell Biol. 113, 45 (1991) Abstract; Full Text
A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53: F. Kappeler, et al.; J. Biol. Chem. 269, 6279 (1994) Abstract; Full Text
Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway: C. Itin, et al.; J. Cell Biol. 131, 57 (1995) Abstract; Full Text
ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins: C. Itin, et al.; Mol. Biol. Cell 7, 483 (1996) Abstract; Full Text
The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII: F. Kappeler, et al.; J. Biol. Chem. 272, 31801 (1997) Abstract; Full Text
Abnormal acidification of melanoma cells induces tyrosinase retention in the early secretory pathway: R. Halaban, et al.; J. Biol. Chem. 277, 14821 (2002) Abstract; Full Text
pH-induced conversion of the transport lectin ERGIC-53 triggers glycoprotein release: C. Appenzeller-Herzog, et al.; J. Biol. Chem. 279, 12943 (2004) Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004) Abstract; Full Text
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005) Abstract; Full Text
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008) Abstract

General Literature References

ERGIC-53 and traffic in the secretory pathway: H.P. Hauri, et al.; J. Cell Sci. 113, 587 (2000) Abstract; Full Text

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-604

Revised 31-Oct-06

Monoclonal Antibody to CLIMP-63 (G1/296)
SYNONYMS	anti-ER Protein p63 MAb (G1/296) anti-Cytoskeleton-linking Membrane Protein of 63kDa MAb (G1/296)
PRODUCT LINE	Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY	ER-Golgi Intermediate Compartment [ERGIC] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-604-C100		100 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Monkey
CLONE:	G1/296
ISOTYPE:	Mouse IgG2a
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	>95%
PURITY DETAIL:	Protein G-affinity purified and gel filtrated (FPLC).
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with 100µl PBS containing 0.1% sodium azide.
IMMUNOGEN:	Human CLIMP-63 (cytoskeleton-linking membrane protein of 63kDa) from Caco-2 cells golgi membrane fraction.
SPECIFICITY:	Recognizes human CLIMP-63. Cross-reacts with monkey CLIMP-63.
APPLICATION:	ELISA Flow Cytometry Immunocytochemistry (1:1’000) Immunohistochemistry (frozen sections) Immunoprecipitation (2µl) Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Description

The cytoskeleton-linking membrane protein of 62kDa (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.

Product Specific Literature References

Characterization of a novel 63 kDa membrane protein. Implications for the organization of the ER-to-Golgi pathway: A. Schweizer, et al.; J. Cell Sci. 104, 671 (1993) Abstract; Full Text
A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein: A. Schweizer, et al.; J. Cell Sci. 104, 685 (1993) Abstract; Full Text
Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers: A. Schweizer, et al.; J. Cell Biol. 126, 25 (1994) Abstract; Full Text
Reassessment of the subcellular localization of p63: A. Schweizer, et al.; J. Cell Sci. 108, 2477 (1995) Abstract; Full Text
A novel direct interaction of endoplasmic reticulum with microtubules: D.R. Klopfenstein, et al.; EMBO J. 17, 6168 (1998) Abstract; Full Text
Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment: D.R. Klopfenstein, et al.; J. Cell Biol. 153, 1287 (2001) Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004) Abstract; Full Text

Further Categories Containing This Product:

Golgi Apparatus • Monoclonal Antibodies

ALX-804-812

Revised 20-Jun-08

Monoclonal Antibody to BAP31 (CC-1)
SYNONYMS	anti-B Cell Receptor-associated Protein BAP31 MAb (CC-1) anti-BCR-associated Protein BAP31 MAb (CC-1) anti-p28 BAP31 MAb (CC-1) anti-CDM Protein MAb (CC-1) anti-6C6-AG Tumor-associated Antigen MAb (CC-1) anti-DXS1357E MAb (CC-1)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	B Cell Receptor [BCR] & Co-Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-812-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Goat Sheep Monkey Others
CLONE:	CC-1
ISOTYPE:	Rat IgG2a
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	0.5mg/ml
PURITY:	>95% (SDS-PAGE)
FORMULATION:	Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:	Solubilized membranes of the IMR-32 human neuroblastoma cell line.
SPECIFICITY:	Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. Does not recognize mouse and rat BAP31.
APPLICATION:	ELISA (capture) Immunocytochemistry (1:1'000-1:10'000; works on endogenous) Immunohistochemistry (frozen sections (1:1'000-1:10'000); works on endogenous) Immunoprecipitation Western Blot (1:1'000-1:6'000; works on endogenous).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells: H.A. Manley and V.A. Lennon; J. Histochem. Cytochem. 49, 1235 (2001) Abstract; Full Text
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils: K. Zen, et al.; J. Biol. Chem. 279, 44924 (2004) Abstract; Full Text
Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules: J.J. Ladasky, et al.; J. Immunol. 177, 6172 (2006) Abstract

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000) Abstract;