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Nitrotyrosine / Related Products
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ALX-106-020 Revised 28-Jan-05
3-Nitro-L-tyrosine
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-106-020-G001   1 g 25.00 USD Add To Cart
ALX-106-020-G005   5 g 90.00 USD Add To Cart
Product Specification
FORMULA: C9H10N2O5
MW: 226.2
CAS NUMBER: 621-44-3
PURITY: ≥98%
APPEARANCE: Yellow to yellow-green powder.
SOLUBILITY: Soluble in acidic aqueous solution (e. g. 1N hydrochloric acid).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C

Product Description
Nitrotyrosine is the major product from the spontaneous reaction of peroxynitrite with tyrosine. Formation of nitrotyrosine in vivo can indicate the formation of peroxynitrite by a nitric oxide (NO)-dependent oxidative damage.
Product Specific Literature References
Peroxynitrite-mediated tyrosine nitration catalyzed by superoxide dismutase: H. Ischiropoulos, et al.; Arch. Biochem. Biophys. 298, 431 (1992) Abstract
Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry: J.S. Beckman, et al.; Biol. Chem. Hoppe-Seyler 375, 81 (1994) Abstract
Peroxynitrite-mediated oxidative protein modifications: H. Ischiropoulos & A.B. Al-Mehdi; FEBS Lett. 364, 279 (1995) Abstract
Detection and quantitation of nitrotyrosine residues in proteins: in vivo marker of peroxynitrite: J.P. Crow, et al.; Meth. Enzymol. 269, 185 (1996) Abstract
Antibodies that recognize nitrotyrosine: Y.Z. Ye, et al.; Meth. Enzymol. 269, 201 (1996) Abstract
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Special Amino Acids
 
 
ALX-840-013 Revised 26-Aug-08
Nitrotyrosine-modified BSA (Positive Control for Prod. No. ALX-804-204)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-840-013-C200   200 µg 94.00 USD Add To Cart
Product Specification
CONCENTRATION: 0.4mg/ml.
FORMULATION: Liquid. In TBS, pH 7.4.
APPLICATION: Positive control for Western blot (50ng/lane) application using MAb to nitrotyrosine (CC22.8C7) (Prod. No. ALX-804-204).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Amount of protein loaded may need to be adjusted depending on the antibody concentration, the detection method utilized (i.e. ECL or color precipitation), and the amount of time required to visualize nitrotyrosine from experimental samples on the film or membrane. E. g., at an antibody concentration of 1mg/ml, 50 ng of peroxynitrite-treated BSA forms a dark band using the NBT/BCIP detection method.
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Positive Controls (Proteins)
 
 
ALX-840-014 Revised 27-Nov-07
Nitrotyrosine-modified BSA (Positive Control for Prod. No. ALX-804-208)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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ALX-840-014-1   1 Vial 269.00 USD Add To Cart
Product Specification
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.09% sodium azide.
APPLICATION: Western Blot (50ng/lane (mini gel) for HRPO / ECL detection)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1 year after receipt when stored at -20°C. Thaw aliquots at +37°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
Further Categories Containing This Product:
Positive Controls (Proteins)
 
 
ALX-804-204 Revised 07-Oct-08
Monoclonal Antibody to Nitrotyrosine (CC22.8C7)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-204-C050   50 µg 126.00 USD Add To Cart
ALX-804-204B-C100 Biotin 100 µg 357.00 USD Add To Cart
ALX-804-204-C200   200 µg 448.00 USD Add To Cart
Product Specification
CLONE: CC22.8C7
ISOTYPE: Mouse IgG2b
CONCENTRATION:

 
FORMULATION: CC22.8C7: Liquid. In PBS, pH 7.2, containing 50% glycerol, 0.1mg/ml BSA and 0.02% sodium azide.
CC22.8C7-Biotin:  Liquid. In PBS, pH 7.2, containing 0.5mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN: Peroxynitrite-treated KLH (keyhole limpet hemocyanin).
SPECIFICITY: Recognizes nitrotyrosine-containing proteins.
APPLICATION: ELISA [1]
Immunohistochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1-2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: CC22.8C7: Stable for at least 2 years after receipt when stored at -20°C.
CC22.8C7-Biotin:  Stable for at least 1 year after receipt when stored at +4°C.
POSITIVE CONTROL: For Positive Control see Prod. No. ALX-840-013.
Product Specific Literature References
[1] Enzyme immunoassays for the investigation of protein nitration by air pollutants: T. Franze, et al.; Analyst. 128, 824 (2003) Abstract
General Information
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide (6.7 x 109/M/sec) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including atherosclerotic lesions of human coronary arteries, postischemic heart, and placenta during preeclampsia. Increased nitration of proteins in motor neurons has been identified in patients with ALS (amyotrophic lateral sclerosis) and may be due to mutations in superoxide dismutase.
General Literature References
Oxidative chemistry of peroxynitrite: J.S. Beckman, et al.; Meth. Enzymol. 233, 229 (1994) Abstract
Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry: J.S. Beckman, et al.; Biol. Chem. Hoppe-Seyler 375, 81 (1994) Abstract
Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly: J.S. Beckman and W.H. Koppenol; Am. J. Physiol. 271, C1424 (1996) Abstract
Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury: P. Wang and J.L. Zweier; J. Biol. Chem. 271, 29223 (1996) Abstract; Full Text
Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amytrophic lateral sclerosis: An immunohistochemical study: S.M. Chou, et al.; J. Chem. Neuroanat. 10, 249 (1996) Abstract
Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action: L. Myatt, et al.; Hypertension 28, 488 (1996) Abstract
Increased 3-nitrotyrosine and oxidative damage in mice with a human copper/zinc superoxide dismutase mutation: R.J. Ferrante, et al.; Ann. Neurol. 42, 326 (1997) Abstract
Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis: K. Abe, et al.; Neurol. Res. 19, 124 (1997) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-208 Revised 03-Jul-07
Monoclonal Antibody to Nitrotyrosine (NOY-7A5)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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ALX-804-208-C100   100 µg 380.00 USD Add To Cart
Product Specification
CLONE: NOY-7A5
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: KLH (keyhole limpet hemocyanin) modified with nitrotyrosine.
SPECIFICITY: Recognizes nitrotyrosine-modified proteins.
APPLICATION: Western Blot (use 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL: For Positive Control see Prod. No. ALX-840-014.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-416 Revised 11-Sep-08
Monoclonal Antibody to Nitrotyrosine [Affinity Sorbent] (CC22.8C7)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
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ALX-804-416-C200   200 µg 403.00 USD Add To Cart
Product Specification
CLONE: CC22.8C7
ISOTYPE: Mouse IgG
FORMULATION: 200µg of anti-Nitrotyrosine MAb conjugated to 200µl of protein A agarose. Supplied in 200μl TBS, pH 7.4.
IMMUNOGEN: Peroxynitrite treated KLH (keyhole limpet hemocyanin).
SPECIFICITY: Recognizes nitrotyrosine-containing proteins.
APPLICATION: Immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Typical procedure for Immunoprecipitation:

1. Dilute the protein sample (cell lysate, tissues homogenate, plasma, etc.) to ~1mg/ml in TBS or PBS in a
     microcentrifuge tube.
2. Add 20-40µl of gel slurry to 0.5-1ml (0.5-1mg protein) and incubate 1-2 hours at room temperature or
     4°C overnight with occasional mixing or gentle rocking.
3. Collect the beads by brief centrifugation (<1 minute) in a microcentrifuge. Carefully drain or pipet off the
     supernatant and wash the beads 3 times with TBS or PBS.
4. Resuspend the beads in 25-50µl of 1X Laemmli sample buffer and boil for 5 minutes. (Alternatively the
     samples can be frozen for later use).
5. Briefly centrifuge the sample to pellet the beads. The supernatant can now be directly used for
     SDS-PAGE.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-505 Revised 16-Jan-06
Monoclonal Antibody to 3-Nitrotyrosine (39B6)
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SYNONYMS anti-3NTyr MAb (39B6)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitrotyrosine / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-505-C050   50 µg 98.00 USD Add To Cart
ALX-804-505-C200   200 µg 230.00 USD Add To Cart
Product Specification
CLONE: 39B6
ISOTYPE: Mouse IgG2a
CONCENTRATION: 0.5mg/ml
FORMULATION: Liquid. In PBS containing 0.09% sodium azide.
IMMUNOGEN: 3-(4-Hydroxy-3-nitrophenylacetamido) propionic acid-BSA conjugate.
SPECIFICITY: Recognizes 3-nitrotyrosine moieties. Not species specific.
APPLICATION: ELISA (with antigen coated plates)
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot (1:5'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control see Prod. No. ALX-840-014.
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Product Specific Literature References
Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC: I. Girault, et al.; Free Radical Biology & Medicine 31, 1375 (2001) Abstract
Inducible Nitric Oxide Synthase and Nitrotyrosine in Listeric Encephalitis: A Cross-species Study in Ruminants: H. Pfister, et al.; Vet. Pathology 39, 190 (2002) Abstract; Full Text
Comparison of nitrotyrosine antibodies and development of immunoassays for the detection of nitrated proteins: T. Franze, et al.; Analyst 129, 589 (2004) Abstract
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
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