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ALX-620-060
Revised 25-Sep-08
DAF-2T
SYNONYMS
Diaminofluorescein-2T
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-060-C100
100 µg
160.00 USD
Product Specification
FORMULA:
C
20
H
11
N
3
O
5
MW:
373.3
CONCENTRATION:
~0.5mM
PURITY:
≥98% (HPLC)
FORMULATION:
Pale yellow liquid. In 0.54ml DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:
After opening, prepare aliquots and store at -20°C. Protect from light. Keep under inert gas.
Product Images
Please click on thumbnails to enlarge.
Product Description
Fluorescent compound (Ex(max): 495nm; Em(max): 515nm) produced by the reaction between DAF-2 (Prod. No.
ALX-620-052
) and nitric oxide (NO). Control compound for NO detection using DAF-2 (Prod. No.
ALX-620-052
) or DAF-2 DA (cell permeable) (Prod. No.
ALX-620-056
).
Not for sale in Japan.
Product Specific Literature References
Fluorescent indicators for imaging nitric oxide production:
H. Kojima, et al.; Angew. Chem. Int. Ed. Engl.
38
, 3209 (1999)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-070
Revised 25-Sep-08
DAF-FM
SYNONYMS
Diaminofluorescein-FM
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-070-M001
1 mg
495.00 USD
Product Specification
FORMULA:
C
21
H
14
F
2
N
2
O
5
MW:
412.3
CONCENTRATION:
~7mM
PURITY:
≥98% (HPLC)
FORMULATION:
Liquid. In 0.35ml DMSO.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Prepare 1’000-fold dilution (~7µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:
Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Product Description
Highly sensitive fluorescent probe (Ex: 500nm; Em: 515nm) for the detection of nitric oxide (NO). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable.
Not for sale in Japan.
Product Specific Literature References
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry:
Y. Itoh, et al.; Anal. Biochem.
287
, 203 (2000)
Abstract
Fluorescent indicators for nitric oxide
:
H. Kojima & N. Nagano; Advanced Materials
12
, 763 (2000)
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore:
H. Kojima, et al.; Anal. Chem.
73
, 1967 (2001)
Abstract
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia:
H. Kojima, et al.; J. Neurochem.
76
, 1404 (2001)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-071
Revised 25-Sep-08
DAF-FM DA (cell permeable)
SYNONYMS
Diaminofluorescein-FM . diacetate
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-071-M001
1 mg
495.00 USD
Product Specification
FORMULA:
C
25
H
18
F
2
N
2
O
7
MW:
496.4
CONCENTRATION:
~5mM
PURITY:
≥98% (HPLC)
FORMULATION:
Liquid. In 0.4ml DMSO.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:
Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.
Product Description
Cell permeable analog of DAF-FM (Prod. No.
ALX-620-070
), that is hydrolyzed to DAF-FM by intracellular esterases (Ex: 500nm; Em: 515nm). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable.
Not for sale in Japan.
Product Specific Literature References
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry:
Y. Itoh, et al.; Anal. Biochem.
287
, 203 (2000)
Abstract
Fluorescent indicators for nitric oxide
:
H. Kojima & T. Nagano; Advanced Materials
12
, 763 (2000)
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia:
H. Kojima, et al.; J. Neurochem.
76
, 1404 (2001)
Abstract
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore:
H. Kojima, et al.; Anal. Chem.
73
, 1967 (2001)
Abstract
Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe:
S. Lepiller et al.; Free Radic. Biol. Med.
43
, 619 (2007)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-610-023
Revised 10-Jul-08
DAN-1 EE . hydrochloride
SYNONYMS
4-((3-Amino-2-naphthalenyl)aminomethyl)benzoic acid ethyl ester . HCl
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-610-023-M005
5 mg
162.00 USD
Product Specification
FORMULA:
C
20
H
20
N
2
O
2
. HCl
MW:
320.4 . 36.5
PURITY:
≥95%
FORMULATION:
Liquid. Solution in ethanol.
SOLUBILITY:
30mg/ml soluble in DMSO or dimethyl formamide; slightly soluble in PBS, pH 7.2 (0.5mg/ml).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.
Product Description
Fluorescent indicator for the bioimaging of nitric oxide (NO). Upon entry into the cell, DAN-1 EE is transformed by cytosolic esterases into the less cell permeable DAN-1. Intracellular formation of NO can be monitored (Ex(max): 360-380nm; Em(max): 420-450nm).
Product Specific Literature References
Development of a fluorescent indicator for the bioimaging of nitric oxide:
H. Kojima, et al.; Biol. Pharm. Bull.
20
, 1229 (1997)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-064
Revised 17-Dec-04
Dansyl-NECA
SYNONYMS
1-Deoxy-1-[6-[[6-[[[5-(dimethylamino)-1-naphthalenyl]sulfonyl]amino]hexyl]amino]-9
H
-purin-9-yl]-N-ethyl-β-D-ribofuranuronamide
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Adenosine Receptors / Related Products
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ALX-620-064-MC05
0.5 mg
80.00 USD
Product Specification
FORMULA:
C
30
H
40
N
8
O
6
S
MW:
640.8
CAS NUMBER:
219982-12-4
PURITY:
≥98%
APPEARANCE:
Yellow solid.
SOLUBILITY:
Soluble in DMSO or methanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Protect from light.
Product Description
Fluorescent ligand for adenosine receptors with a marked selectivity for the A
1
receptor subtype. Has been successfully applied in fluorescence microscopy assays.
Product Specific Literature References
Fluorescent probes for adenosine receptors: synthesis and biology of N6-dansylaminoalkyl-substituted NECA derivatives:
M. Macchia, et al.; Bioorg. Med. Chem. Lett.
8
, 3223 (1998)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-067
Revised 25-Sep-08
DAR-4M
SYNONYMS
Diaminorhodamine-4M
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-067-M001
1 mg
495.00 USD
Product Specification
FORMULA:
C
25
H
26
N
4
O
3
MW:
430.5
CONCENTRATION:
~5mM
PURITY:
≥98% (HPLC)
FORMULATION:
Pale yellow liquid. In DMSO.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:
After opening, prepare aliquots and store at -20°C. Keep under inert gas. Protect from light. Warm to room temperature before opening.
HAZARD:
IRRITANT.
Product Description
Fluorescent probe (Ex: ~560nm; Em: 575nm) for the detection of nitric oxide (NO). It can be used to detect NO directly in biopsy tissues, cultured cells, and tissue sections. Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM.
Not for sale in Japan.
Product Specific Literature References
Fluorescent indicators for nitric oxide:
H. Kojima & N. Nagano ; Advanced Materials
12
, 763 (2000)
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore:
H. Kojima, et al.; Anal. Chem.
73
, 1967 (2001)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-069
Revised 25-Sep-08
DAR-4M AM (cell permeable)
SYNONYMS
Diaminorhodamine-4M AM
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-620-069-M001
1 mg
530.00 USD
Product Specification
FORMULA:
C
28
H
31
N
4
O
5
. I
MW:
503.6 . 126.9
CONCENTRATION:
~5mM
PURITY:
≥
98% (HPLC)
FORMULATION:
Red liquid. In 0.32ml DMSO.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING:
Keep under inert gas. Protect from light. After opening, prepare aliquots and store at -20°C. Warm to room temperature before opening.
HAZARD:
IRRITANT.
Product Description
Cell permeable analog of DAR-4M (Prod. No.
ALX-620-067
). Can be used to detect nitric oxide (NO) directly in cell specimens prepared from various sources, such as the brain (Ex: ~560nm; Em: 575nm). Stable in a pH range of 4-12. Low decrease in fluorescence intensity over time. Detection limit 7nM.
Not for sale in Japan.
Product Specific Literature References
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore:
H. Kojima, et al.; Anal. Chem.
73
, 1967 (2001)
Abstract
Fluorescent indicators for nitric oxide:
H. Kojima & N. Nagano ; Advanced Materials
12
, 763 (2000)
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-620-062
Revised 01-Jun-06
2-(4-Dehydroxy)coelenterazine
SYNONYMS
Coelenterazine
h
PRODUCT LINE
Oxidative Stress
PRODUCT CATEGORY
Peroxynitrite / Scavengers / Detection
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ALX-620-062-C050
50 µg
110.00 USD
Product Specification
FORMULA:
C
26
H
21
N
3
O
2
MW:
407.5
PURITY:
≥95%
APPEARANCE:
Yellow to orange solid.
SOLUBILITY:
Soluble in 100% ethanol or methanol; slightly soluble in water. Avoid DMSO as product is unstable in this solvent.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light. Keep under inert gas. Keep calcium free when stored in solution.
HAZARD:
IRRITANT.
Product Description
Derivative of coelenterazine (Prod. No.
ALX-620-063
) with ~20 times higher luminescence intensity.
Product Specific Literature References
Semi-synthetic aequorins with improved sensitivity to Ca2+ ions:
O. Shimomura, et al.; Biochem. J.
261
, 913 (1989)
Abstract
Preparation and handling of aequorin solutions for the measurement of cellular Ca2+:
O. Shimomura; Cell Calcium
12
, 635 (1991)
Abstract
Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins:
M.R. Knight, et al.; J. Cell. Biol.
121
, 83 (1993)
Abstract
Intracellular Ca2+ signals in Dictyostelium chemotaxis are mediated exclusively by Ca2+ influx:
T. Nebl & P.R. Fisher; J. Cell Sci.
110
, 2845 (1997)
Abstract
Further Categories Containing This Product:
Calcium Indicators / Probes
•
Antioxidants, Flavonoids & Free Radical Scavengers Other Products
•
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
•
Oxidative Stress Markers & Reagents / Related Products
ALX-610-001
Revised 23-Feb-07
2,3-Diaminonaphthalene
SYNONYMS
DAN
2,3-Naphthalenediamine
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitric Oxide & NOS Detection / Related Products
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ALX-610-001-M100
100 mg
40.00 USD
Product Specification
FORMULA:
C
10
H
10
N
2
MW:
158.2
CAS NUMBER:
771-97-1
PURITY:
≥95% (GC)
APPEARANCE:
Off-white to light brown solid.
SOLUBILITY:
Soluble in pyridine, DMSO or dimethylformamide.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HAZARD:
MAY BE CARCINOGENIC. TOXIC.
Product Description
Inhibitor of nitric oxide (NO) formation. The relatively nonfluorescent DAN reacts rapidly with the NO-derived N-nitrosating agent(s) to yield the highly fluorescent product 2,3-naphthotriazole (NAT).
Product Specific Literature References
A fluorometric assay for the measurement of nitrite in biological samples:
T.P. Misko, et al.; Anal. Biochem.
214
, 11 (1993)
Abstract
Determination of nitric oxide using fluorescence spectroscopy:
A.M. Miles, et al.; Meth. Enzymol.
268
, 105 (1996)
Abstract
Determination of selectivity of reactive nitrogen oxide species for various substrates:
D.A. Wink, et al.; Meth. Enzymol.
268
, 120 (1996)
Abstract
Intensity-independent fluorometric detection of cellular nitric oxide release:
P.J. Andrew, et al.; FEBS Lett.
408
, 319 (1997)
Abstract
Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and S-nitrosothiols:
M. Marzinzig, et al.; Nitric Oxide
1
, 177 (1997)
Abstract
Improved nitric oxide detection using 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors:
N. Nakatsubo, et al.; Biol. Pharm. Bull.
21
, 1247 (1998)
Abstract
The oxidative and nitrosative chemistry of the nitric oxide/superoxide reaction in the presence of bicarbonate:
D. Jourd'heuil, et al.; Arch. Biochem. Biophys.
365
, 92 (1999)
Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
ALX-610-022
Revised 25-Apr-08
2',7'-Dichlorodihydrofluorescein diacetate
SYNONYMS
DCDHF diacetate
2',7'-Dichlorofluorescein diacetate
PRODUCT LINE
Other Products
PRODUCT CATEGORY
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-022-M050
50 mg
43.00 USD
Product Specification
FORMULA:
C
24
H
16
O
7
Cl
2
MW:
487.3
CAS NUMBER:
4091-99-0
PURITY:
≥95%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
25mg/ml soluble in DMSO, 100% ethanol or dimethyl formamide; sparingly soluble in aqueous buffers.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stock solution in organic solvents is stable for at least 6 months if stored at -20°C. For maximum solubility in aqueous buffers, dissolve directly in 0.1M disodium carbonate (7.7mg/ml) and then immediately diluted with PBS, pH 7.2, to the desired concentration or pH. Upon neutralization of the solution the concentration may not be greater than 1mg/ml. We do not recommend storing the aqueous solution for more than one day.
Product Description
Cell permeable, sensitive indicator of peroxynitrite formation. After hydrolysis of the diacetate groups by cytosolic esterases or base-catalyzed cleavage of the diacetate groups, DCDHF is oxidized by peroxynitrite to the highly fluorescent product dichlorofluorescein (DCF). Formation of DCF can be monitored by fluorescence spectroscopy (Ex(max): 502nm, Em(max): 523nm), or by absorbance spectroscopy at 500nm (ε=79,500M
-1
cm
-1
). Neither nitric oxide, superoxide nor hydrogen peroxide alone appear to oxidize DCDHF.
Product Specific Literature References
Peroxynitrite-mediated oxidation of dihydrorhodamine 123:
N.W. Kooy, et al.; Free Radic. Biol. Med.
16
, 149 (1994)
Abstract
Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species:
J.P. Crow; Nitric Oxide
1
, 145 (1997)
Abstract
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate:
S.L. Hempel, et al.; Free Radic. Biol. Med.
27
, 146 (1999)
Abstract
Detection of reactive nitrogen species using 2,7-dichlorodihydrofluorescein and dihydrorhodamine 123:
H. Ischiropoulos, et al.; Meth. Enzymol.
301
, 367 (1999)
Abstract
Further Categories Containing This Product:
Peroxynitrite / Scavengers / Detection
ALX-610-021
Revised 04-Nov-05
Dihydrorhodamine 123
