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Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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ALX-420-030 Revised 24-Sep-08
BAY 41-2272
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SYNONYMS 3-(4-Amino-5-cyclopropylpyrimidine-2-yl)-1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridine
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-420-030-M005   5 mg 92.00 USD Add To Cart
ALX-420-030-M025   25 mg 358.00 USD Add To Cart
Product Specification
FORMULA: C20H17FN6
MW: 360.4
CAS NUMBER: 256376-24-6
PURITY: ≥98%
APPEARANCE: White to off-white needles.
SOLUBILITY: Soluble in DMSO, dichloromethane, 100% ethanol or 2-pyrrolidone; practically insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: IRRITANT.

Product Description
NO-independent activator of soluble guanylyl cyclase (sGC) [1,2]. Activates both isoforms α1β1 and α1β2 of sGC [3]. While the related compound YC-1 (Prod. No. ALX-420-025) also acts as a non-specific phosphodiesterase inhibitor, BAY 41-2272 has no effect on phosphodiesterases. Stimulation of sGC is not blocked by high concentrations of NO scavengers (e.g. PTIO, Prod. No. ALX-430-007) and the combination of  BAY 41-2272 with the NO donor DEA NONOate (Prod. No. ALX-430-034) potentiates the activation of sGC. Although BAY 41-2272 alone is not as strong a stimulator of sGC as NO, concentrations as low as 10-100nM stimulate sGC to a level that would be expected to cause biologically important increases in cGMP [1]. ODQ (Prod. No. ALX-270-034), a potent and selective inhibitor of sGC, completely inhibited the effect of BAY 41-2272 [1]. The activity of BAY 41-2272 is described in selected literature references [4-6].
Product Specific Literature References
[1] NO-independent regulatory site on soluble guanylate cyclase: J.P. Stasch, et al.; Nature 410, 212 (2001) Abstract
[2] NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272: E.M. Becker, et al.; BMC Pharmacol. 1, 13 (2001) Abstract; Full Text
NO-independent stimulators of soluble guanylate cyclase: A. Straub, et al.; Bioorg. Med. Chem. Lett. 11, 781 (2001) Abstract
[3] BAY 41-2272 activates two isoforms of nitric oxide-sensitive guanylyl cyclase: M. Koglin, et al.; BBRC 292, 1057 (2002) Abstract
[4] Cardiorenal and humoral properties of a novel direct soluble guanylate cyclase stimulator BAY 41-2272 in experimental congestive heart failure: G. Boerrigter, et al.; Circulation 107, 686 (2003) Abstract
[5] BAY 41-2272: a stimulator of soluble guanylyl cyclase induces nitric oxide-dependent penile erection in vivo: E. Bischoff, et al.; Urology 61, 464 (2003) Abstract
[6] Macrophage endothelial nitric oxide synthase auto-regulates cellular activation and pro-inflammatory protein expression: L. Connelly, et al.; J. Biol. Chem 278, 26480 (2003) Abstract; Full Text
Antiinflammatory activity of soluble guanylate cyclase: cGMP-dependent down-regulation of P-selectin expression and leukocyte recruitment: A. Ahluwalia, et al.; PNAS 101, 1386 (2004) Abstract; Full Text
Identification of residues crucially involved in soluble guanylate cyclase activation: C. Rothkegel, et al.; FEBS Lett. 580, 4205 (2006) Abstract
 
 
ALX-152-022 Revised 04-Jul-03
BPDEtide
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKG Substrates
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-152-022-M001   1 mg 60.00 USD Add To Cart
ALX-152-022-M005   5 mg 240.00 USD Add To Cart
Product Specification
SEQUENCE: H-Arg-Lys-Ile-Ser-Ala-Ser-Glu-Phe-Asp-Arg-Pro-Leu-Arg-OH
FORMULA: C68H115N23O20
MW: 1574.8
PURITY: ≥97%
APPEARANCE: White lyophilized powder.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Substrate for cGMP-dependent protein kinase corresponding to a peptide sequence surrounding the phosphorylation site of cGMP-binding cGMP-specific phosphodiesterase.
Product Specific Literature References
Studies of two different intrachain cGMP-binding sites of cGMP- dependent protein kinase: J.D Corbin & S.O. Dřskeland; J. Biol. Chem. 258, 11391 (1983) Abstract; Full Text
A phenylalanine in peptide substrates provides for selectivity between cGMP- and cAMP-dependent protein kinases: J.L. Colbran, et al.; J. Biol. Chem. 267, 9589 (1992) Abstract; Full Text
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products • Peptides
 
 
ALX-153-055 Revised 25-May-07
Carnosine
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SYNONYMS β-Alanyl-L-histidine
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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ALX-153-055-G001   1 g 55.00 USD Add To Cart
Product Specification
SEQUENCE: H-β-Ala-His-OH
FORMULA: C9H14N4O3
MW: 226.2
CAS NUMBER: 305-84-0
PURITY: ≥98%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in 80% acetic acid (10mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Antioxidant. Inhibits soluble guanylyl cyclase (sGC) by interacting with the heme iron to form a chelate complex.
Product Specific Literature References
Carnosine as a regulator of soluble guanylate cyclase: I.S. Severina, et al.; Biochemistry (Mosc) 65, 783 (2000) Abstract; Full Text
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Antioxidants, Flavonoids & Free Radical Scavengers Other Products
 
 
ALX-430-086 Revised 13-Dec-06
DD1
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SYNONYMS 3-Bromo-3,4,4-trimethyl-3,4-dihydrodiazete 1,2-dioxide
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide Donors
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ALX-430-086-M010   10 mg 30.00 USD Add To Cart
ALX-430-086-M050   50 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C5H9BrN2O2
MW: 209.0
PURITY: ≥98%
APPEARANCE: Crystals.
SOLUBILITY: Soluble in DMSO, 100% ethanol or water (10mM).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Guanylyl cyclase activator. Cell permeable thiol-induced nitric oxide donor with strong vasodilator effects.
Product Specific Literature References
Kinetics of nitric oxide liberation by 3,4-dihydro-1,2-diazete 1,2- dioxides and their vasodilatory properties in vitro and in vivo: D.I. Utepbergenov, et al.; BBRC 214, 1023 (1995) Abstract
Thiol-induced nitric oxide release from 3-halogeno-3,4-dihydrodiazete 1,2-dioxides: I.A. Kirilyuk, et al.; J. Med. Chem. 41, 1027 (1998) Abstract
Geldanamycin leads to superoxide formation by enzymatic and non-enzymatic redox cycling. Implications for studies of Hsp90 and endothelial cell nitric-oxide synthase: S. Dikalov, et al.; J. Biol. Chem. 277, 25480 (2002) Abstract; Full Text
Inflammatory cytokines induce apoptosis of corneal endothelium through nitric oxide: P. Sagoo, et al.; Invest. Ophthalmol. Vis. Sci. 45, 3964 (2004) Abstract; Full Text
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
 
 
ALX-430-087 Revised 23-Feb-05
DD2
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SYNONYMS 3-Bromo-4-methyl-3,4-hexamethylene-3,4-dihydrodiazete 1,2-dioxide
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide Donors
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ALX-430-087-M010   10 mg 30.00 USD Add To Cart
ALX-430-087-M050   50 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C7H11BrN2O2
MW: 235.1
PURITY: ≥98%
APPEARANCE: Crystals.
SOLUBILITY: Soluble in water (3.5mM), DMSO or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Guanylyl cyclase activator. Cell permeable thiol-induced nitric oxide donor with strong vasodilator effects.
Product Specific Literature References
Kinetics of nitric oxide liberation by 3,4-dihydro-1,2-diazete 1,2- dioxides and their vasodilatory properties in vitro and in vivo: D.I. Utepbergenov, et al.; BBRC 214, 1023 (1995) Abstract
Thiol-induced nitric oxide release from 3-halogeno-3,4-dihydrodiazete 1,2-dioxides: I.A. Kirilyuk, et al.; J. Med. Chem. 41, 1027 (1998) Abstract
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
 
 
ALX-850-005 Revised 24-Feb-05
cGMP EIA Kit
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY PKG Kits
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ALX-850-005-KI01   1 Kit 412.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the measurement of cGMP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products • ELISA & EIA Kits
 
 
ALX-480-059 Revised 09-Mar-06
Guanosine 3',5'-cyclic Monophosphate . sodium salt
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SYNONYMS cGMP . Na
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY cGMP Derivatives
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ALX-480-059-M025   25 mg 25.00 USD Add To Cart
ALX-480-059-M100   100 mg 95.00 USD Add To Cart
Product Specification
FORMULA: C10H11N5O7P . Na
MW: 344.2 . 23.0
CAS NUMBER: 40732-48-7
MERCK INDEX: 14: 2709
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Intracellular messenger implicated in mediating action of nitric oxide (NO) in many cell types. Target receptors for the second messenger guanosine 3',5'-cyclic monophosphate (cGMP) include protein kinase G (PKG) isozymes, cGMP-dependent ion channels as well as cGMP-responsive phosphodiesterases. In presence of corresponding phosphodiesterases cGMP is metabolized rapidly. Suppresses apoptosis.
Product Specific Literature References
Cyclic GMP metabolism and involvement in biological regulation: N.D. Goldberg & M.K. Haddox; Ann. Rev. Biochem. 46, 823 (1977) Abstract
Interleukin-1 beta suppresses apoptosis in rat ovarian follicles by increasing nitric oxide production: S.Y. Chun, et al.; Endocrinology 136, 3120 (1995) Abstract
Further Categories Containing This Product:
Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products • Apoptosis Inducers & Inhibitors Other Products
 
 
ALX-157-010 Revised 24-Feb-05
Guanylin (human)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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ALX-157-010-MC01   0.1 mg 115.00 USD Add To Cart
ALX-157-010-M001   1 mg 450.00 USD Add To Cart
Product Specification
SEQUENCE: H-Pro-Gly-Thr-Cys-Glu-Ile-Cys-Ala-Tyr-Ala-Ala-Cys-Thr-Gly-Cys-OH
(Disulfide bonds between Cys4-Cys12 and Cys7-Cys15).
FORMULA: C58H87N15O21S4
MW: 1458.7
PURITY: ≥96%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Endogenous intestinal guanylyl cyclase activator.
Product Specific Literature References
Guanylin: an endogenous activator of intestinal guanylate cyclase: M.G. Currie, et al.; PNAS 89, 947 (1992) Abstract
Precursor structure, expression, and tissue distribution of human guanylin: F.J. de Sauvage, et al.; PNAS 89, 9089 (1992) Abstract
Human guanylin: cDNA isolation, structure, and activity: R.C. Wiegand, et al.; FEBS Lett. 311, 150 (1992) Abstract
Further Categories Containing This Product:
Peptides
 
 
ALX-157-011 Revised 24-Feb-05
Guanylin (rat, mouse)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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ALX-157-011-MC01   0.1 mg 115.00 USD Add To Cart
ALX-157-011-M001   1 mg 450.00 USD Add To Cart
Product Specification
SEQUENCE: H-Pro-Asn-Thr-Cys-Glu-Ile-Cys-Ala-Tyr-Ala-Ala-Cys-Thr-Gly-Cys-OH
(Disulfide bonds between Cys4-Cys12 and Cys7-Cys15).
FORMULA: C60H90N16O22S4
MW: 1515.8
CAS NUMBER: 144940-98-7
PURITY: ≥99%
APPEARANCE: White lyophilized powder.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Endogenous intestinal guanylyl cyclase activator.
Product Specific Literature References
Guanylin: an endogenous activator of intestinal guanylate cyclase: M.G. Currie, et al.; PNAS 89, 947 (1992) Abstract
Cloning and expression of guanylin. Its existence in various mammalian tissues: S. Schulz, et al.; J. Biol. Chem. 267, 16019 (1992) Abstract; Full Text
Rat guanylin cDNA characterization of the precursor of an endogenous activator of intestinal guanylate cyclase: R.C. Wiegand, et al.; BBRC 185, 812 (1992) Abstract
Human guanylin: cDNA isolation, structure, and activity: R.C. Wiegand, et al; FEBS Lett. 311, 150 (1992) Abstract
Further Categories Containing This Product:
Peptides
 
 
ALX-850-241 Revised 25-Jul-06
Guanylyl Cyclase (α1β1), Soluble Detection Set - Replaced by Prod. No. ALX-210-786
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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not sold in USA
Related Products
Further Categories Containing This Product:
Sets Other Products
 
 
ALX-201-180 Revised 29-Jul-08
Guanylyl Cyclase (α1β1), Soluble (human) (recombinant) (His) (enriched)
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SYNONYMS GTP Pyrophosphate-lyase (cycling), Soluble (human) (recombinant) (His) (enriched)
sGCα1/sGCβ1 (human) (recombinant) (His) (enriched)
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Guanylyl Cyclases, Guanosine Phosphate Metabolism / Related Products
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ALX-201-180-R050   50 µl 240.00 USD Add To Cart
Product Specification
EC: 4.6.1.2
SOURCE/HOST: Produced in Sf9 cells. Fused at the C-terminus to a His-tag.
CONCENTRATION: ≥1.5mg/ml (total protein)
FORMULATION: Liquid. Enriched fraction in 50mM TEA, pH 7.4 containing 1mM EDTA/EGTA, 5mM GSH and 30% glycerol.
SPECIFIC ACTIVITY: - Basal: ≥0.001µmol/mg/min.
- 200µM DEA NONOate (Prod. No. ALX-430-034): ≥0.5µmol/mg/min.
- 1µM BAY41-2272 (Prod. No. ALX-420-030): ≥0.01µmol/mg/min.
APPLICATION: Enzymatic studies and drug screening.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: We do not recommend to store for longer periods after opening and thawing of the product.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981) Abstract
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998) Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999) Abstract
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000) Abstract; Full Text
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Prepared by one column purification.

Swiss-prot link:
Human α1 subunit-accession AAB94794, locus CYG3_human
Human β1 subunit-accession AAH47620, locus CYG1_human