• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
Protein Synthesis, Modification & Degradation
You are here: Product Lines > Protein Synthesis, Modification & Degradation
 
Toolbar - View Selection
 
 Items 180-200 of 608 Page 10 of 31 Select Page: << 6 7 8 9 10 11 12 13 14 15  >>  
APO-54N-039 Revised 10-Oct-08 New product
MDA5 (human) (IntraCellular) ELISA Kit
Add to Clipboard
SYNONYMS Melanoma Differentiation-associated Gene 5 (human) (IntraCellular) ELISA Kit
Helicard (human) (IntraCellular) ELISA Kit
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-039-KI01   1 Kit 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 0.4ng/ml (range 0.5 to 32ng/ml).
QUANTITY: For 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human MDA5 in cell extracts.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
NF-kB Pathway Other Products • Antiviral Signalling / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • ELISA & EIA Kits
 
 
ALX-260-091 Revised 10-Apr-08
MG-115
Add to Clipboard
SYNONYMS Z-LL-Nva-CHO
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY Proteasome / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-260-091-M001   1 mg 52.00 USD Add To Cart
ALX-260-091-M005   5 mg 153.00 USD Add To Cart
Product Specification
SEQUENCE: Z-Leu-Leu-Nva-CHO
FORMULA: C25H39N3O5
MW: 461.6
PURITY: ≥95% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Potent, reversible proteasome inhibitor. Specifically inhibits the chymotrypsin-like activity of the proteasome.
Product Specific Literature References
Inhibition of the chymotrypsin-like activity of the pituitary multicatalytic proteinase complex: A. Vinitsky, et al.; Biochemistry 31, 9421 (1992) Abstract
The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B: V.J. Palombella, et al.; Cell 78, 773 (1994) Abstract
Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules: K.L. Rock, et al.; Cell 78, 761 (1994) Abstract
Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae: D.H. Lee & A.L. Goldberg; J. Biol. Chem. 271, 27280 (1996) Abstract; Full Text
p53-dependent induction of apoptosis by proteasome inhibitors: U.G. Lopes, et al.; J. Biol. Chem. 272, 12893 (1997) Abstract; Full Text
Proteasome inhibition results in increased poly-ADP-ribosylation: implications for neuron death: J.N. Keller & W.R. Markesbery; J. Neurosci. Res. 61, 436 (2000) Abstract
Role of proteasomal degradation in the cell cycle-dependent regulation of DNA topoisomerase IIalpha expression: L. Salmena, et al.; Biochem. Pharmacol. 61, 795 (2001) Abstract
 
 
ALX-260-092 Revised 05-Jun-08
MG-132
Add to Clipboard
SYNONYMS Z-LLL-CHO
Z-Leu-Leu-Leu-CHO
PRODUCT LINE Ubiquitin & Proteasome
PRODUCT CATEGORY Proteasome / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-260-092-M001   1 mg 25.00 USD Add To Cart
ALX-260-092-M005   5 mg 60.00 USD Add To Cart
Product Specification
SEQUENCE: Z-Leu-Leu-Leu-CHO
FORMULA: C26H41N3O5
MW: 475.6
CAS NUMBER: 133407-82-6
PURITY: ≥95% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
IDENTITY: Identity determined by MS.
Product Description
Potent, reversible and cell permeable proteasome inhibitor. Inhibits NF-κB activation.
Product Specific Literature References
Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules: K.L. Rock, et al.; Cell 78, 761 (1994) Abstract
Multiple proteolytic systems, including the proteasome, contribute to CFTR processing: T.J. Jensen, et al.; Cell 83, 129 (1995) Abstract
The proteasome pathway is required for cytokine-induced endothelial-leukocyte adhesion molecule expression: M.A. Read, et al.; Immunity 2, 493 (1995) Abstract
Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae: D.H. Lee and A.L. Goldberg; J. Biol. Chem. 271, 27280 (1996) Abstract; Full Text
The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol: E.J. Wiertz, et al.; Cell 84, 769 (1996) Abstract
Proteasome inhibitors activate stress kinases and induce Hsp72. Diverse effects on apoptosis: A.B. Meriin, et al.; J. Biol. Chem. 273, 6373 (1998) Abstract; Full Text
Role of proteasomal degradation in the cell cycle-dependent regulation of DNA topoisomerase IIalpha expression: L. Salmena, et al.; Biochem. Pharmacol. 61, 795 (2001) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors
 
 
ALX-201-065 Revised 18-Sep-08
MGMT (human) (recombinant)
Add to Clipboard
SYNONYMS O6-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-065-R050   50 µl 320.00 USD Add To Cart
Product Specification
EC: 2.1.1.63
SOURCE/HOST: Produced in E. coli. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY: Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION: Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY: 100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O6-methylguanine from DNA.
QUALITY CONTROL: The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P16455: MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-380-026 Revised 26-Jun-08
Monensin . sodium salt
Add to Clipboard
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Ionophores
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-026-M100   100 mg 25.00 USD Add To Cart
ALX-380-026-G001   1 g 63.00 USD Add To Cart
Product Specification
FORMULA: C36H61O11 . Na
MW: 669.9 . 23.0
CAS NUMBER: 22373-78-0
MERCK INDEX: 14: 6246
PURITY: ≥90%
APPEARANCE: White to light yellow powder.
SOLUBILITY: Soluble in chloroform or DMSO. Clear solution (5%) in methanol with heat. Slightly soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Antibiotic that functions as Na+ ionophore. Blocks glycoprotein secretion. Inhibits proliferation of several lymphoma cell lines through cell cycle arrest and apoptosis.
Product Specific Literature References
Monensin, a new biologically active compound. I. Discovery and isolation: M. E. Haney, Jr. & M. M. Hoehn; Antimicrobial Agents Chemother. (Bethesda) 7, 349 (1967) Abstract
Solution chemistry of monensin and its alkali metal ion complexes. Potentiometric and spectroscopic studies
: P.G. Gertenbach & A.I. Popov; JACS 97, 4738 (1975)
Undersulfated proteoglycans are secreted by cultured chondrocytes in the presence of the ionophore monensin: K. Tajiri, et al.; J. Biol. Chem. 255, 6036 (1980) Abstract; Full Text
Expression of membrane IGM by a human B lymphoblastoid cell line in the presence of monensin: R.T. Kubo and M.L. Pigeon; Mol. Immunol. 20, 345 (1983) Abstract
Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity: H. H. Mollenhauer, et al.; Biochim. Biophys. Acta 1031, 225 (1990), Review Abstract
Monensin-mediated growth inhibition in human lymphoma cells through cell cycle arrest and apoptosis: W. H. Park, et al.; Br. J. Haematol. 119, 400 (2002) Abstract
Further Categories Containing This Product:
Cell Cycle Blockers & Inhibitors / Related Products • Vesicles & Membrane Trafficking Other Products • Natural Products for Cell Cycle Research • Antibiotics - Apoptosis Inducers & Inhibitors
 
 
ALX-804-137 Revised 07-Dec-05
Monoclonal Antibody to A20 (human) (8E8.38)
Add to Clipboard
PRODUCT LINE Inflammation
PRODUCT CATEGORY TLRs Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-137-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 8E8.38
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Zinc finger domain corresponding to aa 375-537 of human A20.
SPECIFICITY: Recognizes human A20.
APPLICATION: Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Description
A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4 [1].  A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2 [2-4], which interfere with NF-kB activation upstream of the IKK complex.
Product Specific Literature References
A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome: F.S. Zetoune, et al.; Cytokine 15, 282 (2001) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein: S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun. 3303, 586 (2003) Abstract
[2] The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN: K. Heyninck, et al.; J. Cell Biol. 145, 1471 (1999) Abstract; Full Text
[3] Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2: S. Van Huffel, et al.; J Biol Chem 276, 30216 (2001) Abstract; Full Text
[4] Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1: K. Heyninck, et al.; FEBS Lett. 536, 135 (2003) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products
 
 
ALX-804-391 Revised 02-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (10D8)
Add to Clipboard
SYNONYMS anti-GlcNAc MAb (10D8)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-391-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 10D8
ISOTYPE: Mouse IgM
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: N-Acetylglucosamine.
SPECIFICITY: Recognizes human, mouse and rat N-acetylglucosamine.
APPLICATION: Western Blot (1 µg/ml for AP/BCIP/NBT (MTT) detection; 0.5 µg/ml for HRPO/ECL detection).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-276 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (HGAC85)
Add to Clipboard
SYNONYMS anti-Glc-NAc (O-linked) MAb (HGAC85)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-276-C100   100 µg 379.00 USD Add To Cart
Product Specification
CLONE: HGAC85
ISOTYPE: Mouse IgG3
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Heat killed, pepsin treated group A streptococci.
SPECIFICITY: Recognizes β-1,3 linked O-GlcNAc (O-linked N-acetylglucosamine) residues of streptococcal group A carbohydrates as well as O-GlcNAc glycosylated proteins.
APPLICATION: ELISA
Immunocytochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1µg/ml)
Note: Do not use with diluents containing glycosylated proteins!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier: X. Zhang & V. Bennett; J. Biol. Chem. 271, 31391 (1996) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-111 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)
Add to Clipboard
SYNONYMS anti-Glc-NAc (O-linked) MAb (RL2)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-111-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: RL2
ISOTYPE: Mouse IgG1
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Pore complex-lamina fraction purified from rat liver nuclear envelopes.
SPECIFICITY: Recognizes human, mouse and rat nuclear pore complex (NPC), cytoplasmic and intracellular O-GlcNAc glycosylated proteins. Detects bands of ~210, ~180, ~145, ~100, ~63, ~58, ~54 and ~45kDa by Western blot.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (1:200))
Immunoprecipitation
Western Blot (1:1’000)
Note: Do not use with diluents containing glycosylated proteins.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies identify a group of nuclear pore complex glycoproteins: C.M. Snow, et al.; J. Cell. Biol. 104, 1143 (1987) Abstract
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1: G. Majumdar, et al.; Diabetes 53, 3184 (2004) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION By Western blot, ALX-804-111 recognizes up to eight different proteins from the nuclear pore complex (NPC) of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-027 Revised 11-Jan-05
Monoclonal Antibody to α-Adaptin (AC1-M11)
Add to Clipboard
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-027-R500   500 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Monkey
Others
CLONE: AC1-M11
ISOTYPE: Mouse IgG2a
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified adaptors.
SPECIFICITY: Recognizes human, mouse, rat, pig, monkey, bovine, chicken and frog α-adaptin. Recognizes the products of both α-adaptin genes, α-A and α-C as well as an alternatively spliced isoform of α-A found in neurons.
APPLICATION: Immunocytochemistry (1:100)
Immunoprecipitation
Western Blot (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies: M.S. Robinson; J. Cell. Biol. 104, 887 (1987) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies