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Oxidative Stress
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ALX-350-028 Revised 12-Sep-08
Curcumin (high purity)
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SYNONYMS 1,7-bis(4-Hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione
Diferuloylmethane
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-028-M010   10 mg 15.00 USD Add To Cart
ALX-350-028-M050   50 mg 60.00 USD Add To Cart
ALX-350-028-M250   250 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C21H20O6
MW: 368.4
CAS NUMBER: 458-37-7
MERCK INDEX: 14: 2673
SOURCE/HOST: Isolated from turmeric (Curcuma longa).
PURITY: ≥98.5% (Note: This highly purified product is free of demethoxy- and bis-demethoxycurcumin and does not contain 30-40% bioactive impurities)
APPEARANCE: Orange-yellow crystalline powder.
SOLUBILITY: Soluble in acetic acid or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HANDLING: Protect from light.

Product Description
Dual inhibitor of cyclooxygenase (COX) and 5-lipoxygenase. Also inhibits glutathione S-transferase, induction of nitric oxide (NO) in activated macrophages and inhibits EGF-induced tyrosine phosphorylation of EGF receptors. Antioxidant. Inhibitor of NAD(P)H:quinone oxidoreductase 1 (NQO1), disrupting the binding of NQO1 to wild type p53 inducing ubiquitin-independent degradation of p53 and inhibits p53-mediated apoptosis in normal thymocytes and myeloid leukemic cells.
Product Specific Literature References
Modification of certain inflammation-induced biochemical changes by curcumin: R. Srivastava & R.C. Srimal; Indian J. Med. Res. 81, 215 (1985) Abstract
Inhibition of 5-hydroxy-eicosatetraenoic acid (5-HETE) formation in intact human neutrophils by naturally-occurring diarylheptanoids: inhibitory activities of curcuminoids and yakuchinones: D.L. Flynn, et al.; Prostagl. Leukotr. Med. 22, 357 (1986) Abstract
Inhibitory effect of curcumin on epidermal growth factor receptor kinase activity in A431 cells: L. Korutla & R. Kumar; Biochim. Biophys. Acta 1224, 597 (1994) Abstract
Curcumin, an anti-tumour promoter and anti-inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages: I. Brouet & H. Okshima; BBRC 206, 533 (1995) Abstract
Cytotoxicity and cytoprotective activities of natural compounds. The case of curcumin: J.N. Commandeur & N.P. Vermeulen; Xenobiotica 26, 667 (1996) Abstract
Nitric oxide scavenging by curcuminoids: Sreejayan & M.N. Rao; J. Pharm. Pharmacol. 49, 105 (1997) Abstract
Inhibitory effects of curcumin on tumorigenesis in mice: M.T. Huang, et al.; J. Cell. Biochem. (Suppl.) 27, 26 (1997) Abstract
In vivo inhibition of nitric oxide synthase gene expression by curcumin, a cancer preventive natural product with anti-inflammatory properties: M.M. Chan, et al.; Biochem. Pharmacol. 55, 1955 (1998) Abstract
Effect of curcumin on the production of nitric oxide by cultured rat mammary gland: M. Onoda & H. Inano; Nitric Oxide 4, 505 (2000) Abstract
Suppression of nitric oxide oxidation to nitrite by curcumin is due to the sequestration of the reaction intermediate nitrogen dioxide, not nitric oxide: B.D. Johnston & E.G. DeMaster; Nitric Oxide 8, 231 (2003) Abstract
Biological properties of curcumin-cellular and molecular mechanisms of action: B. Joe, et al.; Crit. Rev. Food Sci. Nutr. 44, 97 (2004), (Review) Abstract
Curcumin, a major constituent of turmeric, corrects cystic fibrosis defects: M.E. Egan, et al.; Science 304, 600 (2004) Abstract
Inhibition of NAD(P)H:quinone oxidoreductase 1 activity and induction of p53 degradation by the natural phenolic compound curcumin: P. Tsvetkov, et al.; PNAS 102, 5535 (2005) Abstract
Immunomodulatory effects of curcumin: V.S. Yadav, et al.; Immunopharmacol. Immunotoxicol. 27, 485 (2005) Abstract
Curcumin, an atoxic antioxidant and natural NFkappaB, cyclooxygenase-2, lipooxygenase, and inducible nitric oxide synthase inhibitor: a shield against acute and chronic diseases: S. Bengmark; JPEN J. Parenter. Enteral. Nutr. 30, 45 (2006), (Review) Abstract
Curcumin is an inhibitor of p300 histone acetylatransferase: M.G. Marcu, et al.; Med. Chem. 2, 169 (2006) Abstract
Multiple biological activities of curcumin: a short review: R.K. Maheshwari, et al.; Life Sci. 78, 2081 (2006), (Review) Abstract
Curcumin inhibits hypoxia-induced angiogenesis via down-regulation of HIF-1: M.K. Bae, et al.; Oncol. Rep. 15, 1557 (2006) Abstract
Notch-1 down-regulation by curcumin is associated with the inhibition of cell growth and the induction of apoptosis in pancreatic cancer cells: Z. Wang, et al.; Cancer 106, 2503 (2006) Abstract
Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species: M. Panchatcharam, et al.; Mol. Cell Biochem. 290, 87 (2006) Abstract
Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia: K.K. Jung, et al.; Life Sci. 79, 2022 (2006) Abstract
The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats: T. Morimoto, et al.; J. Clin. Invest. 118, 868 (2008) Abstract; Full Text
 
 
ALX-202-022 Revised 01-Oct-07
Cu/Zn-Superoxide Dismutase (bovine liver)
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SYNONYMS SOD (Cu/Zn) (bovine liver)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Superoxide Dismutases [SODs] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-202-022-UT50   5 KU 40.00 USD Add To Cart
ALX-202-022-5T50   5x5 KU 160.00 USD Add To Cart
ALX-202-022-UZ50   50 KU 170.00 USD Add To Cart
ALX-202-022-5Z50   5x50 KU 660.00 USD Add To Cart
Product Specification
MW: ~31kDa.
CAS NUMBER: 9054-89-11
EC: 1.15.1.1
SOURCE/HOST: Isolated from bovine liver.
FORMULATION: Lyophilized. Essentially salt free.
RECONSTITUTION: Reconstitute with distilled water, saline or neutral buffer to give a clear slightly blue solution.
SPECIFIC ACTIVITY: ~4'600U/mg protein. One unit is defined as the amount of enzyme that inhibits the rate of reduction of ferricytochrome c by 50% at 25°C, pH 7.8 (J.M. McCord & I. Fridovich; J. Biol. Chem. 244, 6049 (1969)).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY:

Reconstituted product should be stored at +4° and is stable for up to 2 days.

HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P00442: Cu/Zn Superoxide Dismutase (bovine)
Further Categories Containing This Product:
Natural ProteinsEnzymes
 
 
ALX-850-033 Revised 17-Apr-08
Cu/Zn-Superoxide Dismutase ELISA Kit
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SYNONYMS SOD (Cu/Zn) ELISA Kit
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Superoxide Dismutases [SODs] / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-033-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.04ng/ml (range 0.08 to 5ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human Cu/Zn SOD in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Late stages of apoptosis are characterized by the loss of membrane integrity and a release of the intracellular Cu/Zn SOD.
Product Specific Literature References
A highly sensitive non-radioactive cytotoxicity assay for human target cells: L. Franke & T. Porstmann; J. Immunol. Methods 171, 259 (1994) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-385-003 Revised 03-Apr-08
Cyanidin chloride
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SYNONYMS 3,3',4',5,7-Pentahydroxy-flavylium chloride
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Anthocyanidins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-385-003-M010   10 mg 195.00 USD Add To Cart
ALX-385-003-M050   50 mg 780.00 USD Add To Cart
Product Specification
FORMULA: C15H11ClO6
MW: 322.7
CAS NUMBER: 528-58-5
MERCK INDEX: 14: 2687
RTECS: LK9824000
PURITY: ≥97%
APPEARANCE: Dark red to black solid.
SOLUBILITY: Soluble in methanol or 0.1% hydrochloric acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
IDENTITY: Identity determined by 1H-NMR, 13C-NMR and MS.

Product Description
Antioxidant flavonoid. Nitric oxide (NO) scavenger.
Product Specific Literature References
Flavonoids as scavengers of nitric oxide radical: S.A.B.E. Acker, et al.; BBRC 214, 755 (1995) Abstract
Structure-antioxidant activity relationships of flavonoids and phenolic acids: C.A. Rice-Evans, et al.; Free Radical Biol. & Med. 20, 933 (1996), (Review) Abstract
Dietary flavonoids: bioavailability, metabolic effects, and safety: J.A. Ross & C.M. Kasum; Annu. Rev. Nutr. 22, 19 (2002), (Review) Abstract
Cyanidins: metabolism and biological properties: F. Galvano, et al.; J. Nutr. Biochem. 15, 2 (2004), (Review) Abstract
 
 
ALX-850-261 Revised 11-Apr-08
Cytochrome c (human) ELISA Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytochrome c
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-261-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.05ng/ml (range 0.08 to 5ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human cytochrome c in cell culture lysates, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps of apoptosis initiation in cells. Cytochrome c release in the cytosol occurs prior to the activation of caspases and DNA fragmentation which is considered the hallmark of apoptosis.
General Information
Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Cytochrome c release in the cytosol occurs prior to the activation of caspases and DNA fragmentation which is considered the hallmark of apoptosis.
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
Mitochondria-based Apoptosis DetectionELISA & EIA Kits
 
 
ALX-850-277 Revised 03-Feb-05
Cytochrome c Releasing Apoptosis Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytochrome c
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-277-KI01   1 Kit 539.00 USD Add To Cart
Product Specification
QUANTITY: 100 tests.
KIT/SET CONTAINS: 10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial 500X Protease Inhibitor Cocktail, 0.5ml Cytochrome c Antibody (0.2mg/ml).
APPLICATION: For the detection of cytochrome c translocation from mitochondria into cytosol during apoptosis.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. Cytochrome c released from mitochondria into cytosol is determined by Western blot using the cytochrome c antibody provided in the kit. The procedure is simple, straightforward and requires no ultracentrifugation.
General Information
MANUFACTURER

Manufactured by BioVision.

Further Categories Containing This Product:
Mitochondria-based Apoptosis DetectionDetection Kits
 
 
ALX-350-009 Revised 03-Apr-08
Daidzein
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SYNONYMS 4',7-Dihydroxyisoflavone
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Protein Kinase Inhibitors
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-009-M010   10 mg 20.00 USD Add To Cart
ALX-350-009-M025   25 mg 40.00 USD Add To Cart
ALX-350-009-M050   50 mg 60.00 USD Add To Cart
Product Specification
FORMULA: C15H10O4
MW: 254.2
CAS NUMBER: 486-66-8
MERCK INDEX: 14: 2801
RTECS: DJ3100040
SOURCE/HOST: Synthetic.
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO; slightly soluble in 100% ethanol; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: IRRITANT.

Product Description
Inactive analog of the tyrosine kinase inhibitor genistein (Prod. No. ALX-350-006). Shows anti-inflammatory effect.
Product Specific Literature References
Genistein, a specific inhibitor of tyrosine-specific protein kinases: T. Akiyama, et al.; J. Biol. Chem. 262, 5592 (1987) Abstract; Full Text
Role of tyrosine kinases in IgE-mediated signal transduction in human lung mast cells and basophils: S.E. Lavens, et al.; Am. J. Respir. Cell. Mol. Biol. 7, 637 (1992) Abstract
Tyrosine kinase inhibitors block calcium channel currents in vascular smooth muscle cells: S. Wijetunge, et al.; BBRC 189, 1620 (1992) Abstract
Genistein and daidzein, and their ß-glycoside conjugates: anti-tumor isoflavones in soybean foods from American and Asian diets: L. Coward, et al.; J. Agric. Food Chem. 41, 1961 (1993)
Stimulation of pS2 expression by diet-derived compounds: N. Sathyamoorthy, et al.; Cancer Res. 54, 957 (1994) Abstract
Daidzein inhibits insulin- or insulin-like growth factor-1-mediated signaling in cell cycle progression of Swiss 3T3 cells: K. Higashi and H. Ogawara; Biochim. Biophys. Acta 1221, 29 (1994) Abstract
Decreased circulating levels of tumor necrosis factor-alpha in postmenopausal women during consumption of soy-containing isoflavones: Y. Huang, et al.; J. Clin. Endocrinol. Metab. 90, 3956 (2005) Abstract
Gut bacterial metabolism of the soy isoflavone daidzein: exploring the relevance to human health: C. Atkinson, et al.; Exp. Biol. Med. (Maywood) 230, 155 (2005), Review Abstract
Clinical review: a critical evaluation of the role of soy protein and isoflavone supplementation in the control of plasma cholesterol concentrations: A. Dewell, et al.; J. Clin. Endocrinol. Metab. 91, 772 (2006), Review Abstract
Phytoestrogens and lipoproteins in women: C.N. Bairey Merz, et al.; J. Clin. Endocrinol. Metab. 91, 2209 (2006) Abstract
 
 
ALX-350-248 Revised 23-Jun-05
Daidzin
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SYNONYMS Daidzein-7-O-glucoside
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Chemopreventive Agents
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-248-M002   2 mg 75.00 USD Add To Cart
ALX-350-248-M010   10 mg 290.00 USD Add To Cart
Product Specification
FORMULA: C21H20O9
MW: 416.4
CAS NUMBER: 552-66-9
MERCK INDEX: 14: 2801
PURITY: ≥98%
APPEARANCE: White to beige powder.
SOLUBILITY: Soluble in DMSO or dilute aqueous base; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C

Product Description
Glucoside of the isoflavone daidzein (Prod. No. ALX-350-009) found in soy beans.
Product Specific Literature References
Metabolism of puerarin and daidzin by human intestinal bacteria and their relation to in vitro cytotoxicity: D.H. Kim, et al.; Biol. Pharm. Bull. 21, 628 (1998) Abstract
Daidzin and its antidipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria: W.M. Keung & B.L. Vallee; PNAS 95, 2198 (1998) Abstract
Daidzein and genistein but not their glucosides are absorbed from the rat stomach: M.K. Piskula, et al.; FEBS Lett. 447, 287 (1999) Abstract
Further Categories Containing This Product:
Active Substances from Fruit and VegetablesIsoflavones
 
 
ALX-270-281 Revised 28-Sep-06
Daphnetin
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SYNONYMS 7,8-Dihydroxycoumarin
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY EGFR Kinase Inhibitors
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-281-M005   5 mg 25.00 USD Add To Cart
ALX-270-281-M025   25 mg 95.00 USD Add To Cart
Product Specification
FORMULA: C9H6O4
MW: 178.1
CAS NUMBER: 486-35-1
RTECS: GN6382600
PURITY: ≥97%
APPEARANCE: White to rose powder.
SOLUBILITY: Soluble in 100% ethanol, methanol or dimethyl formamide.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C

Product Description
Inhibitor of protein kinases. EGF receptor tyrosine kinase (IC50=7.87µM), PKA (IC50=9.33µM), PKC (IC50=25.01µM). Antioxidant. Antimalarial agent.
Product Specific Literature References
Daphnetin: a novel antimalarial agent with in vitro and in vivo activity: Y.Z. Yang, et al.; Am. J. Trop. Med. Hyg. 46<