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Quantification Kits
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ALX-850-318 Revised 14-Feb-07
Antioxidant Assay Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents/Related Products
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ALX-850-318-KI01   1 Kit 349.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests)
APPLICATION: For the rapid measurement of antioxidant capacity in a variety of sample types, including plasma, serum, urine, saliva, and cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
This antioxidant assay is designed to measure the overall antioxidant capacity within a given sample. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS in comparison to trolox, a water-soluble tocopherol analog. By quantifying the cumulative effect of all antioxidants present, more relevant biological information is acquired compared with the measurement of individual components alone. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2’-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS®·+ by metmyoglobin.
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Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Quantification Kits
 
 
ALX-850-248 Revised 22-Apr-08
ApoSENSOR™ ADP/ATP Ratio Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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ALX-850-248-KI01   200 tests 455.00 USD Add To Cart
Product Specification
QUANTITY: 200 assays.
KIT/SET CONTAINS: 50ml Nucleotide Releasing Buffer, 1 vial ATP Monitoring Enzyme, 1 vial ADP Converting Enzyme, 500µl Enzyme Reconstitution Buffer.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Protect from light.
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Product Description
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.
This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements. The ADP/ATP ratio assay offers highly consistent results, with excellent correlation to other apoptosis markers (e. g. TUNEL-based techniques and caspase assays).
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MANUFACTURER Manufactured by BioVision.
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Quantification Kits
 
 
ALX-850-247 Revised 22-Apr-08
ApoSENSOR™ Cell Viability Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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ALX-850-247-KI01   200 tests 203.00 USD Add To Cart
ALX-850-247-KI02   1000 tests 609.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Nucleotide Releasing Buffer, ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, ATP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
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Product Description
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
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MANUFACTURER Manufactured by BioVision.
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Quantification Kits
 
 
ALX-850-038 Revised 28-Aug-06
Glutathione Assay Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Glutathione/Related Products
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ALX-850-038-KI01   1 Kit 349.00 USD Add To Cart
Product Specification
SENSITIVITY: 0-16µM GSH (or 0-8µM GSSG)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the measurement of glutathione (GSH) in plasma, tissue samples and cultured cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues: F. Tietze; Anal. Biochem. 27, 502 (1969) Abstract
Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine: O.W. Griffith; Anal. Biochem. 106, 207 (1980) Abstract
Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman's reagent: P. Eyer & D. Podhradsky; Anal. Biochem. 153, 57 (1986) Abstract
Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples: M.A. Baker, et al.; Anal. Biochem. 190, 360 (1990) Abstract
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Quantification Kits
 
 
ALX-850-026 Revised 28-Apr-08
Lipid Hydroperoxide (LPO) Assay Kit (100 determinations)
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Lipid Peroxidation
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ALX-850-026-KI01   1 Kit 368.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.25-5nmole
QUANTITY: 100 tests.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Description
Unlike other methods of lipid peroxidation measurement that rely on decomposition products, such as malondialdehyde (Prod. No. ALX-280-018) and 4-hydroxynonenal (Prod. No. ALX-270-245), this new assay features direct measurement of lipid hydroperoxides via a redox reaction. Any sample containing lipid hydroperoxide can be easily and reliably quantified spectrophotometrically using either glass or quartz cuvettes (λmax: 500nm; ε: 16,667M-1cm-1).
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Quantification Kits
 
 
ALX-850-203 Revised 28-Apr-08
Lipid Hydroperoxide (LPO) Assay Kit (96 wells)
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Lipid Peroxidation
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ALX-850-203-KI01   1 Kit 582.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.25-5nmole
QUANTITY: 96 wells (~80 tests).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Description
Unlike other methods of lipid peroxidation measurement that rely on decomposition products, such as malondialdehyde (Prod. No. ALX-280-018) and 4-hydroxynonenal (Prod. No. ALX-270-245), this new assay features direct measurement of lipid hydroperoxides via a redox reaction. Any sample containing lipid hydroperoxide can be easily and reliably quantified spectrophotometrically using either glass or quartz cuvettes (λmax: 500nm; ε: 16,667M-1cm-1). Contains a reusable glass 96 well plate (for use of organic solvents).
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Quantification Kits
 
 
ALX-850-001 Revised 14-Jan-08
Nitrate/Nitrite Colorimetric Assay Kit
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection/Related Products
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ALX-850-001-KI01   1 Kit 332.00 USD Add To Cart
Product Specification
QUANTITY: 2x96 wells, 3 plates (~160 tests).
APPLICATION: For the colorimetric measurement of total nitric oxide production. This kit has been validated in urine, culture media and plasma.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
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Product Description
Provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process. First step: conversion of nitrate to nitrite utilizing nitrate reductase. Second step: addition of Griess reagents and photometric measurement.

The kit includes sufficient reagents for measuring of total NO (nitrate + nitrite) in 2x96 wells, together with a third 96 well plate for measurement of nitrite only.
Product Specific Literature References
L-arginine transport by the microvillous plasma membrane of the syncytiotrophoblast from human placenta in relation to nitric oxide production: effects of gestation, preeclampsia, and intrauterine growth restriction: P.T. Ayuk, et al.; J. Clin. Endocrinol. Metab. 87, 747 (2002) Abstract
Nitric oxide participates in the intestinal pathology associated with murine syngeneic graft-versus-host disease: D.M. Flanagan, et al.; J. Leukoc. Biol. 72, 762 (2002) Abstract
Heme deficiency may be a factor in the mitochondrial and neuronal decay of aging: H. Atamna, et al.; PNAS 99, 14807 (2002) Abstract
Discoordinate regulation of renal nitric oxide synthase isoforms in ovariectomized mRen2.Lewis rats: L.M. Yamaleyeva, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 292, R819 (2007) Abstract
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Quantification Kits
 
 
ALX-850-022 Revised 24-Feb-05
Nitrate/Nitrite Colorimetric Assay Kit (LDH method)
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection/Related Products
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ALX-850-022-KI01   1 Kit 332.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the measurement of total nitric oxide (NO) production in urine, culture media and plasma.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
The use of LDH that destroys excess NADPH (an essential cofactor of NOS), makes this kit well suited for measurement of NOS activity, particularly in high-throughput screening applications using recombinant NOS.
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Quantification Kits
 
 
ALX-850-012 Revised 24-Feb-05
Nitrate/Nitrite Fluorometric Assay Kit
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection/Related Products
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ALX-850-012-KI01   1 Kit 368.00 USD Add To Cart
Product Specification
QUANTITY: 2 x 96 wells, 3 plates (~160 tests).
APPLICATION: For the fluorometric measurement of total nitric oxide production. The kit has been validated in culture media and plasma.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
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Product Description
Provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process.
First step: conversion of nitrate to nitrite utilizing nitrate reductase.
Second step: addition of DAN reagent and NaOH followed by fluorescent measurement. Lower limit of detection ~0.02µM.

The kit includes sufficient reagents for assay of total NO (nitrate + nitrite) in 2x96 wells, together with a third 96 well plate for measurement of nitrite only.
Product Specific Literature References
A spectrophotometric assay for nitrate using NADPH oxidation by Aspergillus nitrate reductase: M.B. Gilliam, et al.; Anal. Biochem. 212, 359 (1993) Abstract
A fluorometric assay for the measurement of nitrite in biological samples: T.P. Misko, et al.; Anal. Biochem. 214, 11 (1993) Abstract
Fluorimetric Determination of Nitric Oxide: A.M. Miles, et al.; Methods 7, 40 (1995)
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Quantification Kits
 
 
ALX-850-037 Revised 26-Jun-08
Nitroso-thiol (RSNOs) Detection Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents/Related Products
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ALX-850-037-KI01   1 Kit 590.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Store kit at +4°C. Store standard (vial 5) at -20°C
Product Description
The assay of nitroso-thiols in many biological fluids, appears to provide a very useful index of NOS activity. The concentration of RSNOs has been shown to increase with localized induction of NOS and to fall rapidly upon administration of NOS inhibitors.
The kit provides a complete solution for the assay of high or low MW RSNOs. The assay is divided essentially into two steps. In the first step, the sample is prepared according to which subgroup of RSNO is desired. The S-N bond is then cleaved, resulting in the formation of an equivalent of nitrite, which is assayed using the Griess reaction. The intensity of the color formed is linearly related to the concentration of nitrite, and hence, to the original RSNO concentration.
Product Specific Literature References
Increased nitrosothiols in exhaled breath condensate in inflammatory airway diseases: M. Corradi, et al.; Am. J. Respir. Crit. Care Med. 163, 854 (2001) Abstract; Full Text
Dose-dependent onset and cessation of action of inhaled budesonide on exhaled nitric oxide and symptoms in mild asthma: S.A. Kharitonov, et al.; Thorax 57, 889 (2002) Abstract
General Information
MANUFACTURER Manufactured by Oxonon.
General Literature References
Detection of S-nitrosothiols by fluorometric and colorimetric methods: D.A. Wink, et al.; Methods Enzymol. 301, 201 (1999) Abstract
Fluorometric detection of S-nitrosothiols: P. Kostka and J.K. Park; Methods Enzymol. 301, 227 (1999) Abstract
Mechanisms of biological S-nitrosation and its measurement: T. Akaike; Free Radic. Res. 33, 461 (2000) Abstract
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Nitric Oxide & NOS Detection/Related Products • Quantification Kits
 
 
ALX-850-006 Revised 25-May-07
NOSdetect™ Assay Kit
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection/Related Products
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ALX-850-006-KI01   1 Kit 390.00 USD Add To Cart
Product Specification
QUANTITY: 50 assays.
APPLICATION: For the measurement of nitric oxide synthase (NOS) activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
Product Description
Measuring NOS activity by monitoring the conversion of L-arginine to L-citrulline is currently the standard assay for NOS activity in both crude and purified enzyme preparations. Advantages of the NOSdetect™ Assay Kit (Stratagene) include the use of radioactive substrates ([3H]-arginine or [14C]arginine) that enable sensitivity to the picomole level, as well as the specificity of the assay for the NOS pathway due to the direct enzymatic conversion of L-arginine to L-citrulline in eukaryotic cells. Additionally, the easy separation of neutrally charged L-citrulline from positively charged L-arginine allows hundreds of assays to be performed in less than an hour.
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MANUFACTURER Manufactured by Stratagene.
Further Categories Containing This Product:
NOS Other Products • Quantification Kits
 
 
ALX-850-287 Revised 16-Jul-08
OXI-TEK TBARS Assay Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents/Related Products
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ALX-850-287-KI01   1 Kit 416.00 USD Add To Cart
Product Specification
QUANTITY: 160 tests
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress. Malondialdehyde (MDA) forms a 1:2 adduct with thiobarbituric acid; the adduct can be measured by fluorometry. Biological specimens contain a mixture of TBARS, including lipid hydroperoxides and aldehydes. In practice, TBARS are expressed in equivalent numbers to MDA.
Product Specific Literature References
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment: T. Keiper, et al.; FASEB J. 19, 2078 (2005) Abstract; Full Text
Adiponectin mediates the suppressive effect of rosiglitazone on plasminogen activator inhibitor-1 production: R. L. Hoo, et al.; Arterioscler. Thromb. Vasc. Biol. 27, 2777 (2007) Abstract
Dyslipidemia inhibits Toll-like receptor-induced activation of CD8alpha-negative dendritic cells and protective Th1 type immunity: A.T. Shamshiev, et al.; J. Exp. Med. 204, 441 (2007) Abstract; Full Text
Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice: T. Murase, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R281 (2008) Abstract
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