ALEXIS Biochemicals: Monoclonal Antibody to STAT1 (human) (SM2)

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JAK-binding Receptors / Related Products

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ALX-270-168

Revised 18-May-06

Tyrphostin AG 490
SYNONYMS	Tyrphostin B42 N-Benzyl-3,4-dihydroxy-benzylidenecyanoacetamide α-Cyano-(3,4-dihydroxy)-]N-benzylcinnamamide
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Tyrphostins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-168-M005		5 mg	45.00 USD
ALX-270-168-M025		25 mg	190.00 USD

Product Specification

FORMULA:	C₁₇H₁₄N₂O₃
MW:	294.3
CAS NUMBER:	133550-30-8
PURITY:	≥98%
APPEARANCE:	Yellow solid.
SOLUBILITY:	Soluble in DMSO and acetone; slightly soluble in 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Selectively inhibits CDK2 activation leading to a potent cell cycle arrest at late G1 and S phase. Cell permeable inhibitor of JAK-2 tyrosine kinase. Does not significantly inhibit other kinases, such as Lck, Lyn, Btk, Syk or Src. Blocks IL-7 induced JAK kinase activity in T cells (JAK-1, JAK-3) and the consequent phosphorylation of PI-3 kinase. Valuable tool for studying the cellular role of JAK kinases in signal transduction.

Product Specific Literature References

Tyrphostins I: synthesis and biological activity of protein tyrosine kinase inhibitors: A. Gazit, et al.; J. Med. Chem. 32, 2344 (1989) Abstract
Tyrosine phosphorylation is an obligatory event in IL-2 secretion [published erratum appears in J Immunol 1991 May 1;146(9):3260]: A. Levitzki; Biochem. Pharmacol. 40, 913 (1990) Abstract
Activation of phosphatidylinositol-3 kinase by ligation of the interleukin-7 receptor is dependent on protein tyrosine kinase activity: H. Dadi, et al.; Blood 84, 1579 (1994) Abstract; Full Text
JAK3 protein tyrosine kinase mediates interleukin-7-induced activation of phosphatidylinositol-3’ kinase: N. Sharfe, et al.; Blood 86, 2077 (1995) Abstract; Full Text
Inhibition of acute lymphoblastic leukaemia by a Jak-2 inhibitor: N. Meydan, et al.; Nature 379, 645 (1996) Abstract
Tyrphostin AG-490 inhibits cytokine-mediated JAK3/STAT5a/b signal transduction and cellular proliferation of antigen-activated human T cells: R.A. Kirken, et al.; J. Leukoc. Biol. 65, 891 (1999) Abstract
JAK3, STAT, and MAPK signaling pathways as novel molecular targets for the tyrphostin AG-490 regulation of IL-2-mediated T cell response: L.H. Wang, et al.; J. Immunol. 162, 3897 (1999) Abstract
JAK2 tyrosine kinase inhibitor tyrphostin AG490 downregulates the mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription (STAT) pathways and induces apoptosis in myeloma cells: J. De Vos, et al.; Br. J. Haematol. 109, 823 (2000) Abstract

Further Categories Containing This Product:

Janus Kinases [JAKs] / Related Products • Tyrosine Kinase Inhibitors • CDK & Cyclin Inhibitors • Cell Cycle Blockers & Inhibitors / Related Products

ALX-270-425

Revised 10-Apr-08

Indirubin-3’-(2,3-dihydroxypropyl)oximether
SYNONYMS	E804
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Src Family Kinases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-425-M001		1 mg	120.00 USD

Product Specification

FORMULA:	C₁₉H₁₇N₃O₄
MW:	351.4
PURITY:	≥96% (¹H-NMR)
FORMULATION:	Dark red solid.
SOLUBILITY:	Soluble in DMSO (1M).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Inhibitor of c-Src (IC₅₀=0.43µM) blocking downstream STAT3 signalling. Induces apoptosis in human cancer cells.

Product Specific Literature References

Indirubin derivatives inhibit Stat3 signaling and induce apoptosis in human cancer cells: S. Nam, et al.; PNAS 102, 5998 (2005) Abstract

Further Categories Containing This Product:

JAK / STAT Signalling Pathway Other Products • Apoptosis Inducers & Inhibitors Other Products

ALX-270-483

Revised 07-Apr-08

WP1066
SYNONYMS	3-(6-Bromo-2-pyridinyl)-2-cyano-N-[(1S)-1-phenylethyl]-2-propenamide
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Antitumor Agents (Apoptosis Inducers)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-483-M001		1 mg	190.00 USD

Product Specification

FORMULA:	C₁₇H₁₄BrN₃O
MW:	356.2
CAS NUMBER:	857064-38-1
PURITY:	≥95%
APPEARANCE:	Yellow solid.
SOLUBILITY:	Soluble in DMSO (>5mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
IDENTITY:	Identity determined by ¹H-NMR.

Product Description

Cell permeable STAT3 inhibitor. Activates Bax. Shows potent anticancer effect on malignant glioma cells. Induces apoptosis and inhibits the growth of tumors. Acts as a potent immune adjuvant by upregulating CD80 and CD86 and inducing proliferation of effector T cells.

Product Specific Literature References

A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients: S.F. Hussain, et al.; Cancer Res. 67, 9630 (2007) Abstract
A novel inhibitor of the STAT3 pathway induces apoptosis in malignant glioma cells both in vitro and in vivo: A. Iwamaru, et al.; Oncogene 26, 2435 (2007) Abstract

Further Categories Containing This Product:

ALX-159-001

Revised 01-Mar-06

IL-2 Receptor Fragment (human)
SYNONYMS	Interleukin-2 Receptor Fragment (human)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Receptors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-159-001-M001		1 mg	80.00 USD

Product Specification

SEQUENCE:	H-Gln-Arg-Arg-Gln-Arg-Lys-Ser-Arg-Arg-Thr-Ile-OH
FORMULA:	C₅₉H₁₁₃N₂₉O₁₆
MW:	1484.7
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Substrate for protein kinase C (PKC). The peptide is derived from the cytoplasmic C-terminus of the human interleukin-2 receptor.

Product Specific Literature References

Phosphorylation of the human interleukin-2 receptor and a synthetic peptide identical to its C-terminal, cytoplasmic domain: B. Gallis, et al.; J. Biol. Chem. 261, 5075 (1986) Abstract; Full Text

Further Categories Containing This Product:

PKC Substrates • CD25 [IL-2Ralpha] / Related Products • Peptides

ALX-201-367

Revised 07-Oct-08

ST2 (human):Fc (human) (recombinant)
SYNONYMS	IL-1 Receptor-like 1 (human):Fc (human) (recombinant) Interleukin-1 Receptor-like 1 (human):Fc (human) (recombinant) IL1RL1 (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Receptors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-367-C050		50 µg	410.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. The signal peptide and the sequence of isoform 2 of human ST2 (IL1RL1) (aa 1-328) are fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:	0.25mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
BIOLOGICAL ACTIVITY:	Interacts with human IL-33 as confirmed by pull-down assay (see figure 2).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -20°C.

Product Images

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General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q01638: Interleukin-1 receptor-like 1 (human) [Precursor]
MANUFACTURER	Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Interleukin-33 [IL-33] & Receptors / Related Products

ALX-201-370

Revised 07-Oct-08

ST2 (human) (recombinant)
SYNONYMS	IL-1 Receptor-like 1 (human) (recombinant) Interleukin-1 Receptor-like 1 (human) (recombinant) IL1RL1 (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Receptors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-370-C050		50 µg	795.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. The signal peptide and the sequence of isoform 2 of human ST2 (IL1RL1) (aa 1-328) are fused at the C-terminus to a FLAG^®-tag.
CONCENTRATION:	0.5mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Images

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General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q01638: Interleukin-1 receptor-like 1 (human) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Interleukin-33 [IL-33] & Receptors / Related Products

ALX-522-057

Revised 01-Oct-08

IL-1 Receptor Type I (human):Fc (human) (recombinant)
SYNONYMS	IL-1RI (human):Fc (human) (recombinant) Interleukin-1 Receptor Type I (human):Fc (human) (recombinant) CD121a (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Receptors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-057-C050		50 µg	365.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
MW:	~83kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. Human IL-1RI (interleukin-1 receptor type I) (aa 21-336) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water.
SPECIFICITY:	Binds to human IL-1 (interleukin-1).
BIOLOGICAL ACTIVITY:	Blocks IL-1 activity.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Cytokine receptors and signal transduction: A. Miyajima, et al.; Annu. Rev. Immunol. 10, 295 (1992) Abstract
cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily: J.E. Sims, et al.; Science 241, 585 (1988) Abstract
The interleukin-1 receptor/Toll-like receptor superfamily: signal transduction during inflammation and host defense: L.A. O'Neill; Sci. STKE 2000, RE1 (2000) Abstract
IL-1 and IL-18 receptors, and their extended family: J.E. Sims, et al.; Curr. Opin. Immunol. 14, 117 (2002) Abstract

ALX-522-098	IL-33 (human) (recombinant)
ALX-522-101	IL-33 (mouse) (recombinant)
ALX-804-840	Monoclonal Antibody to IL-33 (Nessy-1)
ALX-210-933	Polyclonal Antibody to IL-33 (AT110)

SPECIES CROSSREACTIVITY:	Human
CLONE:	ST33868
ISOTYPE:	Mouse IgG1κ
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant human ST2.
SPECIFICITY:	Recognizes human ST2.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:5’000) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 1-2 weeks when stored at +4°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	Produced in CHO cells. Mouse ST2 (aa 27-332) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to mouse and human IL-33.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

ST2 is a transmembrane receptor of the new IL-1 family member IL-33. It induces the NF-κB pathway upon its activation and is involved in T helper type 2 response driving the production of Th2-associated cytokines. ST2 is predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P14719: Interleukin-1 Receptor-like 1 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

SOURCE/HOST:	Produced in E. coli. Full length human SOCS-3 (suppressor of cytokine signalling-3) (aa 1-225) is fused at the C-terminus to a His-tag.
CONCENTRATION:
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in 20mM sodium phosphate, pH 6.5, containing 20mM sodium chloride, 50mM glutamate, 50mM arginine, 2mM dithiothreitol, 1mM EDTA.
ENDOTOXIN CONTENT:	<1.0EU/µg protein (LAL test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O14543: Suppressor of Cytokine Signaling 3 (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.