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ALX-163-011
Revised 14-Jul-08
NBD Peptide / NF-κB Blocker (cell permeable)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Inhibitors
Ordering Information
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ALX-163-011-1
1 Vial
240.00 USD
Product Specification
SEQUENCE:
GRKKRRQRRR-GG-TALDWSWLQTE-amide
FORMULA:
C
121
H
202
N
48
O
32
MW:
2841.2
QUANTITY:
50µl
CONCENTRATION:
10mM
PURITY:
≥95% (HPLC)
FORMULATION:
Liquid. In sterile PBS.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Protect from moisture. Keep on ice at all times.
POSITIVE CONTROL:
Prod. No.
ALX-168-009/1
Product Description
For increased cell permeability the peptide was covalently linked to the 10 aa recognized by the TAT transporter.
Product Specific Literature References
Selective inhibition of NF-kappaB blocks osteoclastogenesis and prevents inflammatory bone destruction in vivo:
E. Jimi, et al.; Nature Med.
10
, 617 (2004)
Abstract
Selective inhibition of NF-kappaB activation by a peptide that blocks the interaction of NEMO with the IkappaB kinase complex:
M.J. May, et al.; Science
289
, 1550 (2000)
Abstract
Characterization of the Ikappa B-kinase NEMO binding domain:
M.J. May, et al.; J. Biol. Chem.
277
, 45992 (2002)
Abstract
Related Products
ALX-168-009/1
L-TAT Control Peptide (for Prod. No.
ALX-159-600
)
Further Categories Containing This Product:
Peptides
ALX-167-024
Revised 17-May-06
SN50
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Inhibitors
Ordering Information
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ALX-167-024-C500
500 µg
125.00 USD
Product Specification
SEQUENCE:
H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH
FORMULA:
C
129
H
230
N
36
O
29
S
MW:
2781.5
PEPTIDE CONTENT:
50-75%
PURITY:
≥96% (HPLC)
APPEARANCE:
White to off-white solid.
FORMULATION:
Counter Ion:
Trifluoroacetate salt.
SOLUBILITY:
Soluble in distilled water, 100% ethanol or ethanol:water 1:1.
RECONSTITUTION:
Reconstitute in sterile distilled water.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Peptides in solution have limited stability.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Inhibits translocation of the NF-κB active complex into the nucleus. In mouse endothelial LE-II cells induced with LPS, inhibition of NF-κB nuclear translocation was 88% at 50µg/ml (18µM). In human monocytic THP-1 cells induced by LPS or TNF-α there was 85% inhibition at 100µg/ml.
For control peptide, see SN50M (Prod. No.
ALX-167-025
).
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence:
Y.Z. Lin, et al.; J. Biol. Chem.
270
, 14255 (1995)
Abstract
;
Full Text
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism:
K.K. Meja, et al.; Br. J. Pharmacol.
131
, 1143 (2000)
Abstract
Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis:
M. Niwa, et al.; Eur. J. Pharmacol
407
, 211 (2000)
Abstract
Activation of nuclear factor kappaB and induction of apoptosis by tumor necrosis factor-alpha in the mouse uterine epithelial WEG-1 cell line:
S. Pampfer, et al.; Biol. Reprod.
63
, 879 (2000)
Abstract
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells
:
Y. Dai, et al.; Blood
103
, 2761 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Nuclear localization sequence (NLS) aa 360-369 (AAVALLPAVLLALLAPVQRKRQKLMP) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF).
Further Categories Containing This Product:
Peptides
•
Nuclear Envelope & Nuclear Transport
ALX-167-025
Revised 17-May-06
SN50M Control Peptide
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Inhibitors
Ordering Information
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ALX-167-025-C500
500 µg
125.00 USD
Product Specification
SEQUENCE:
H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Pro-Val-Gln-Arg-Asn-Gly-Gln-Lys-Leu-Met-Pro-OH
FORMULA:
C
123
H
215
N
33
O
30
S
MW:
2668.4
PEPTIDE CONTENT:
70-90%
PURITY:
≥97% (HPLC)
APPEARANCE:
White to off-white solid.
FORMULATION:
Counter Ion:
Trifluoroacetate salt.
SOLUBILITY:
Soluble in water.
RECONSTITUTION:
Reconstitute in sterile distilled water.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Peptides in solution have limited stability.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Inactive control peptide for SN50 (Prod. No.
ALX-167-024
). In mouse endothelial LE-II cells, induced with LPS, 50µg/ml SN50M had no measurable effect on NF-κB translocation. In human monocytic THP-1 cells induced by LPS or TNF-α there was slight inhibition at 100µg/ml.
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence
:
Y.Z. Lin, et al.; J. Biol. Chem.
270
, 14255 (1995)
Abstract
;
Full Text
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells
:
Y. Dai, et al.; Blood
103
, 2761 (2004)
Abstract
Further Categories Containing This Product:
Peptides
•
Nuclear Envelope & Nuclear Transport
ALX-201-056
Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
SYNONYMS
Interleukin 1
β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
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ALX-201-056-U025
25 U
231.00 USD
ALX-201-056-U100
100 U
385.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-
p
NA (
p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-
p
NA (Prod. No.
ALX-260-026
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P29466:
Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492:
Caspase-1 (mouse)
Further Categories Containing This Product:
Inflammasome
•
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-094
Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-094-U025
25 U
203.00 USD
ALX-201-094-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P51878:
Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500:
Caspase-5 (mouse)
Further Categories Containing This Product:
Inflammasome
•
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-284
Revised 18-Mar-08
NF-κB (p65) (human) (recombinant) (His)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers:
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ALX-201-284-C002
2 µg
280.00 USD
Product Specification
SOURCE/HOST:
Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION:
0.5mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY:
~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1:
P.O. Hassa, et al.; J. Biol. Chem.
278
, 45145 (2003)
Abstract
;
Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription:
P.O. Hassa, et al.; J. Biol. Chem.
280
, 40450 (2005)
Abstract
;
Full Text
Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression:
M. Covic, et al.; EMBO J.
24
, 85 (2005)
Abstract
;
Full Text
MYBBP1a is a novel repressor of NF-κB:
H. R. Owen, et al.; J. Mol. Biol.
366
, 725 (2007)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q04206:
NF-κB (human) (p65 subunit)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-285
Revised 09-Oct-08
NF-κB (p50) (human) (recombinant) (His)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Transcription Factors / Related Products
Ordering Information
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ALX-201-285-C002
2 µg
280.00 USD
Product Specification
SOURCE/HOST:
Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION:
0.2mg/ml
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 125mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY:
~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1:
P.O. Hassa, et al.; J. Biol. Chem.
278
, 45145 (2003)
Abstract
;
Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription:
P.O. Hassa, et al.; J. Biol. Chem.
280
, 40450 (2005)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P19838:
NF-κB (human) (p50 subunit)
AfCS Signalling Gateway link A002937:
NF-κB (mouse) (p50 subunit)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-408
Revised 27-Mar-08
Ribosomal Protein S3 (human) (recombinant) (His)
SYNONYMS
RPS3 (human) (recombinant) (His)
40S Ribosomal Protein S3
IMR-90 Ribosomal Protein S3
PRODUCT LINE
Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY
Ribosomal Proteins / Related Products
Ordering Information
Product Numbers:
Format:
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Quantity:
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ALX-201-408-C010
10 µg
330.00 USD
ALX-201-408-C050
50 µg
740.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Mature peptide of human ribosomal protein S3 (aa 1-243) is fused at the C-terminus to a His-tag.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in 25mM TRIS-HCl, pH 8.5, containing 1mM DTT.
ENDOTOXIN CONTENT:
<1EU/µg protein (LAL-test).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation:
T.S. Kim, et al.; Mol. Biol. Cell
17
, 824 (2006)
Abstract
;
Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK:
S.H. Kim & J. Kim; Biochim. Biophys. Acta
1763
, 823 (2006)
Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation:
F. Wan, et al.; Cell
131
, 927 (2007)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P23396:
Ribosomal Protein S3 (human)
MANUFACTURER
Manufactured by AdipoGen, Inc.
Related Products
ALX-201-410
Ribosomal Protein S3 (human) (recombinant)
ALX-210-481
Polyclonal Antibody to Ribosomal Protein S3 (human)
ALX-804-733
Monoclonal Antibody to Ribosomal Protein S3 (human) (RP159-1)
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products
•
DNA Endonucleases / Related Products
•
Recombinant Proteins / Fusion Proteins
•
NF-kB Pathway Other Products
•
MAPK Pathway Other Products
ALX-201-410
Revised 13-Feb-08
Ribosomal Protein S3 (human) (recombinant)
PRODUCT LINE
Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY
Ribosomal Proteins / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-410-C010
10 µg
330.00 USD
ALX-201-410-C050
50 µg
740.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. The mature peptide of human ribosomal protein S3 (aa 1-243) is fused at the N-terminus to a tag.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. 0.2µm-filtered solution in 25mM TRIS-HCl, pH 8.5, containing 1mM DTT.
ENDOTOXIN CONTENT:
<1EU/µg protein (LAL-test).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation:
T.S. Kim, et al.; Mol. Biol. Cell
174
, 824 (2006)
Abstract
;
Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK:
S.H. Kim & J. Kim; Biochim. Biophys. Acta
1763
, 823 (2006)
Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation:
F. Wan, et al.; Cell
131
, 927 (2007)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P23396:
Ribosomal Protein S3 (human)
MANUFACTURER
Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products
•
DNA Endonucleases / Related Products
•
Recombinant Proteins / Fusion Proteins
•
NF-kB Pathway Other Products
•
MAPK Pathway Other Products
ALX-210-017
Revised 12-Apr-07
Polyclonal Antibody to NALP1 (human) (CT) (Bur 242)
SYNONYMS
anti-NACHT-, LRR- and PYD-containing Protein 1 (human) (CT) PAb (Bur 242)
anti-DEFCAP (human) (CT) PAb (Bur 242)
anti-NAC (human) (CT) PAb (Bur 242)
anti-CARD7 (human) (CT) PAb (Bur 242)
anti-Caspase Recruitment Domain-containing Protein 7 (human) (CT) PAb (Bur 242)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Inflammasome
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-210-017-R050
50 µl
190.00 USD
Product Specification
