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Oxidative Stress Markers & Reagents / Related Products
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ALX-200-600 Revised 12-Nov-07
Myeloperoxidase (human leukocytes)
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-600-U010   10 U 90.00 USD Add To Cart
ALX-200-600-U025   25 U 165.00 USD Add To Cart
ALX-200-600-5010   5x10 U 350.00 USD Add To Cart
ALX-200-600-5025   5x25 U 650.00 USD Add To Cart
Product Specification
MW: ~118kDa.
CAS NUMBER: 9003-99-0
EC: 1.11.1.7
SOURCE/HOST: Isolated from human leukocytes. Negative for HBsAG-, HCV- and HIV-antibodies.
PURITY: ≥95% (SDS-PAGE). 
FORMULATION: Lyophilized from 0.02M sodium acetate buffer, pH 6.0.
RECONSTITUTION: Reconstitute with 1ml distilled water.
SPECIFIC ACTIVITY: ~250-300U/mg protein. One unit is defined as the amount of enzyme that produces an increase in absorbance at 470nm of 1.0 per min. at 25°C, pH 7.0 (calculated from the initial rate of reaction using 1% guaiacol as substrate; initial H2O2 concentration 6mmol/l).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Specific Literature References
Guinea pig heterophil and eosinophil peroxidase: R.K. Desser, et al.; Arch. Biochem. Biophys. 148, 452 (1972) Abstract
Oxidation of amino acids and peptides in reaction with myeloperoxidase, chloride and hydrogen peroxide: R. Drozdz, et al.; Biochim. Biophys. Acta 957, 47 (1988) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P05164: Myeloperoxidase (human) (precursor)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
ALX-201-342 Revised 18-Mar-08
GPX3 (human) (recombinant)
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SYNONYMS Glutathione Peroxidase 3 (human) (recombinant)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-342-C010   10 µg 295.00 USD Add To Cart
ALX-201-342-C050   50 µg 795.00 USD Add To Cart
Product Specification
EC: 1.11.1.9
SOURCE/HOST: Produced in HEK 293 cells. Mature human GPX3 (glutathione peroxidase 3) is fused at the C-terminus to a FLAG®-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P22352: Glutathione peroxidase 3 (human) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-398 Revised 18-Mar-08
GPX3 (mouse) (recombinant)
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SYNONYMS Glutathione Peroxidase 3 (mouse) (recombinant)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-398-C010   10 µg 270.00 USD Add To Cart
ALX-201-398-C050   50 µg 795.00 USD Add To Cart
Product Specification
EC: 1.11.1.9
SOURCE/HOST: Produced in HEK 293 cells. Mature mouse GPX3 (glutathione peroxidase 3) (aa 1-226) is fused at the C-terminus to a FLAG®-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P46412: Glutathione peroxidase 3 (mouse) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-210-477 Revised 07-Jan-08
Polyclonal Antibody to GPX3
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SYNONYMS anti-Glutathione Peroxidase 3 PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-477-C100   100 µg 340.00 USD Add To Cart
ALX-210-477B-C050 Biotin 50 µg 340.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant mouse GPX3 (glutathione peroxidase 3).
SPECIFICITY: Recognizes mouse GPX3. Weakly cross-reacts with human GPX3.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:5’000)
Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY:

HANDLING: Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-767 Revised 30-Sep-08
Polyclonal Antibody to (E)-4-Hydroxynonenal
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SYNONYMS anti-HNE PAb
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-210-767-R100   100 µl 495.00 USD Add To Cart
Product Specification
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized. Neat serum containing 0.05% sodium azide.
RECONSTITUTION: Reconstitute with 100µl PBS.
IMMUNOGEN: Free recombinant HNE (E)-4-hydroxynonenal.
SPECIFICITY: Recognizes HNE-adducts.
APPLICATION: ELISA (1:1'000-1:10'000)
Immunohistochemistry (1:500-1:1'000)
Western Blot (1:500-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Immunochemical detection of 4-hydroxynonenal protein adducts in oxidized hepatocytes: K. Uchida, et al.; PNAS 90, 8742 (1993) Abstract
Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in human renal-cell carcinoma: K. Okamoto, et al.; Int. J. Cancer 58, 825 (1994) Abstract
Immunocytochemical detection of lipid peroxidation in phagosomes of human neutrophils: correlation with expression of flavocytochrome b: M.T. Quinn, et al.; J. Leukoc. Biol. 57, 415 (1995) Abstract
Characterization of epitopes recognized by 4-hydroxy-2-nonenal specific antibodies: K. Uchida, et al.; Arch. Biochem. Biophys. 324, 241 (1995) Abstract
Immunohistochemical detection of 4-hydroxynonenal protein adducts in Parkinson disease: A. Yoritaka, et al.; PNAS 93, 2696 (1996) Abstract
alpha-Phenyl-n-tert-butyl-nitrone attenuates hypoxic-ischemic white matter injury in the neonatal rat brain: S. Lin, et al.; Brain Res. 1007, 132 (2004) Abstract
Acquired Resistance to Acetaminophen Hepatotoxicity is Associated with Induction of Multidrug Resistance-Associated Protein 4 (Mrp4) in Proliferating Hepatocytes: L.M. Aleksunes, et al.; Toxicol. Sci. 104, 261 (2008) Abstract
Involvement of inducible nitric oxide synthase in hydroxyl radical-mediated lipid peroxidation in streptozotocin-induced diabetes: K. Stadler, et al.; Free Radic. Biol. Med. Epub ahead of print, (2008) Abstract
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Further Categories Containing This Product:
Lipid Peroxidation • Polyclonal Antibodies
 
 
ALX-210-802 Revised 06-Dec-06
Polyclonal Antibody to 8-Hydroxyguanosine
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-210-802-R100   100 µl 210.00 USD Add To Cart
Product Specification
SOURCE/HOST: From goat.
FORMULATION: Liquid. Neat serum containing 0.1% sodium azide.
IMMUNOGEN: Synthetic 8-hydroxyguanosine-conjugate.
SPECIFICITY: Recognizes 8-hydroxyguanosine and 8-hydroxy-2'-deoxyguanosine. Partially cross-reacts with 8-mercaptoguanosine and slightly with 8-bromoguanosine. Does not cross-react with other naturally occuring nucleotides.
APPLICATION: ELISA (1:250'000)
Immunohistochemistry (frozen sections, paraffin sections (1:200))
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging and neurodegenerative conditions, such as Alzheimer's disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.
Further Categories Containing This Product:
Nucleic Acid Oxidation & Alkylation / Related Products • Polyclonal Antibodies
 
 
AG-G0631 Revised 11-Jan-08
GPX3 (human) ELISA Kit
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SYNONYMS Glutathione Peroxidase 3 (human) ELISA Kit
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-G0631EK-KI01   96 wells 590.00 USD Add To Cart
AG-G0631TP-KI01 Twin Plex 2x96 wells 890.00 USD Add To Cart
AG-G0631PP-KI01 Penta Plex 5x96 wells 1'870.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SENSITIVITY: 100pg/ml (range 0 to 64ng/ml)
APPLICATION: For the quantitative determination of GPX3 in human serum, plasma or cell culture supernatants.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
AG-G0832 Revised 22-Apr-08
GPX3 (mouse) ELISA Kit
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SYNONYMS Glutathione Peroxidase 3 (mouse) ELISA Kit
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
AG-G0832EK-KI01   96 wells 620.00 USD Add To Cart
AG-G0832TP-KI01 Twin Plex 2x96 wells 880.00 USD Add To Cart
AG-G0832PP-KI01 Penta Plex 5x96 wells 1'870.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SENSITIVITY: 150pg/ml (range 0 to 64ng/ml)
SPECIFICITY: Does not cross-react with human or rat sera.
APPLICATION: For the quantitative determination of GPX3 in mouse serum, plasma or cell culture supernatants.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-850-037 Revised 26-Jun-08
Nitroso-thiol (RSNOs) Detection Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-850-037-KI01   1 Kit 590.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Store kit at +4°C. Store standard (vial 5) at -20°C
Product Description
The assay of nitroso-thiols in many biological fluids, appears to provide a very useful index of NOS activity. The concentration of RSNOs has been shown to increase with localized induction of NOS and to fall rapidly upon administration of NOS inhibitors.
The kit provides a complete solution for the assay of high or low MW RSNOs. The assay is divided essentially into two steps. In the first step, the sample is prepared according to which subgroup of RSNO is desired. The S-N bond is then cleaved, resulting in the formation of an equivalent of nitrite, which is assayed using the Griess reaction. The intensity of the color formed is linearly related to the concentration of nitrite, and hence, to the original RSNO concentration.
Product Specific Literature References
Increased nitrosothiols in exhaled breath condensate in inflammatory airway diseases: M. Corradi, et al.; Am. J. Respir. Crit. Care Med. 163, 854 (2001) Abstract; Full Text
Dose-dependent onset and cessation of action of inhaled budesonide on exhaled nitric oxide and symptoms in mild asthma: S.A. Kharitonov, et al.; Thorax 57, 889 (2002) Abstract
General Information
MANUFACTURER Manufactured by Oxonon.
General Literature References
Detection of S-nitrosothiols by fluorometric and colorimetric methods: D.A. Wink, et al.; Methods Enzymol. 301, 201 (1999) Abstract
Fluorometric detection of S-nitrosothiols: P. Kostka and J.K. Park; Methods Enzymol. 301, 227 (1999) Abstract
Mechanisms of biological S-nitrosation and its measurement: T. Akaike; Free Radic. Res. 33, 461 (2000) Abstract
Further Categories Containing This Product:
Nitric Oxide & NOS Detection / Related Products • Quantification Kits
 
 
ALX-850-287 Revised 16-Jul-08
OXI-TEK TBARS Assay Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-850-287-KI01   1 Kit 416.00 USD Add To Cart
Product Specification
QUANTITY: 160 tests
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress. Malondialdehyde (MDA) forms a 1:2 adduct with thiobarbituric acid; the adduct can be measured by fluorometry. Biological specimens contain a mixture of TBARS, including lipid hydroperoxides and aldehydes. In practice, TBARS are expressed in equivalent numbers to MDA.
Product Specific Literature References
The role of junctional adhesion molecule-C (JAM-C) in oxidized LDL-mediated leukocyte recruitment: T. Keiper, et al.; FASEB J. 19, 2078 (2005) Abstract; Full Text
Adiponectin mediates the suppressive effect of rosiglitazone on plasminogen activator inhibitor-1 production: R. L. Hoo, et al.; Arterioscler. Thromb. Vasc. Biol. 27, 2777 (2007) Abstract
Dyslipidemia inhibits Toll-like receptor-induced activation of CD8alpha-negative dendritic cells and protective Th1 type immunity: A.T. Shamshiev, et al.; J. Exp. Med. 204, 441 (2007) Abstract; Full Text
Tea catechin ingestion combined with habitual exercise suppresses the aging-associated decline in physical performance in senescence-accelerated mice: T. Murase, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R281 (2008) Abstract
General Information
MANUFACTURER Manufactured by Zeptometrix Corporation.
Further Categories Containing This Product:
Lipid Peroxidation • Quantification Kits