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Oxidative Stress
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ALX-804-031 Revised 14-Dec-06
Monoclonal Antibody to Gp96/GRP94 (9G10)
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SYNONYMS anti-Glycoprotein 96 MAb (9G10)
anti-Glucose Regulated Protein 94 MAb (9G10)
anti-ERp99 MAb (9G10)
anti-Endoplasmin MAb (9G10)
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY Endoplasmatic Reticulum Stress
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-031-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
CLONE: 9G10
ISOTYPE: Rat IgG2a
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Chicken oviduct Gp96 (GRP94).
SPECIFICITY: Recognizes mouse, rat, bovine, chicken and hamster Gp96 (GRP94). Detects a band of ~94kDa by Western blot.
APPLICATION: Immunocytochemistry (1:250)
Immunoprecipitation
Western Blot (1:5’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein: D.P. Edwards, et al.; Biochemistry 23, 4427 (1984) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-063 Revised 30-Nov-05
Monoclonal Antibody to Glutathione S-transferase (4H3)
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SYNONYMS anti-GST MAb (4H3)
PRODUCT LINE Cancer
PRODUCT CATEGORY Glutathione S-Transferase
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ALX-804-063-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Others
CLONE: 4H3
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2 x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water.
IMMUNOGEN: Recombinant Schistosoma japonicum GST (glutathione S-transferase).
SPECIFICITY: Recognizes GST of Schistosoma japonicum that is encoded by the pGEX expression vectors. Does not cross-react with mammalian (human, mouse or rat) GST.
APPLICATION: Immunoprecipitation
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Glutathione / Related Products • Monoclonal Antibodies
 
 
ALX-804-108 Revised 18-Sep-07
Monoclonal Antibody to Ah Receptor Nuclear Translocator (2B10)
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SYNONYMS anti-ARNT MAb (2B10)
anti-HIF-1β MAb (2B10)
anti-Hypoxia-inducible Factor 1β MAb (2B10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-108-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 2B10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 771-789 (N771SYNNEEFPDLTMFPPFSE789) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY: Recognizes human, mouse and monkey ARNT. Detects a band of ~87kDa by Western blot.
APPLICATION: Immunocytochemistry (1:1'000)
Immunoprecipitation
Western Blot (1:4'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor translocator: Characterization of two monoclonal antibodies to the ARNT: N.G. Hord & G. Perdew; Mol. Pharmacol. 46, 618 (1994) Abstract
Oxygen tension modulates beta-globin switching in embryoid bodies: S. Bichet, et al.; FASEB J. 13, 285 (1999) Abstract; Full Text
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell Sci. 112, 1203 (1999) Abstract; Full Text
Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein-mediated transactivation potential in a dimer context: W.P. Long, et al.; J. Biol. Chem. 274, 12391 (1999) Abstract; Full Text
DNA methylation represses the expression of the human erythropoietin gene by two different mechanisms: H. Yin and K.L. Blanchard; Blood 95, 111 (2000) Abstract; Full Text
A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses: Y.S. Chun, et al.; J. Cell Sci. 114, 4051 (2001) Abstract; Full Text
Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression: T. Sanchez-Elsner, et al.; J. Biol. Chem. 276, 38527 (2001) Abstract; Full Text
Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia: H. Suzuki, et al.; Oncogene 20, 5779 (2001) Abstract; Full Text
Rescue of hypoxia-inducible factor-1alpha-deficient tumor growth by wild-type cells is independent of vascular endothelial growth factor: G. Höpfl, et al.; Cancer Res. 62, 2962 (2002) Abstract; Full Text
The arylhydrocarbon receptor (AhR), but not the AhR-nuclear translocator (ARNT), is increased in hearts of patients with cardiomyopathy: M.R. Mehrabi, et al.; Virchows Arch. 441, 481 (2002) Abstract; Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products • Monoclonal Antibodies
 
 
ALX-804-122 Revised 26-Feb-07
Monoclonal Antibody to Cytochrome c (apo) (SJL2-4(apo))
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SYNONYMS anti-Cyt c (apo) MAb (SJL2-4(apo))
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytochrome c
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ALX-804-122-C100   100 µg 260.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Goat
Sheep
Monkey
CLONE: SJL2-4(apo)
ISOTYPE: Mouse IgG2b
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Horse cytochrome c.
SPECIFICITY: Recognizes human, mouse, rat, sheep, goat and monkey apocytochrome c.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C undiluted.
HANDLING: Do not freeze/thaw.
Product Images
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Product Specific Literature References
Differential effects of two anti-apo-cytochrome c-specific monoclonal antibodies on the function of apo-cytochrome c-specific murine T cell clones: G. Corradin, et al.; J. Immunol. 133, 2915 (1984) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997) Abstract
Cytochrome c: can't live with it-can't live without it: J.C. Reed; Cell 91, 559 (1997) Abstract
Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked: J. Yang, et al.; Science 275, 1129 (1997) Abstract
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3: H. Zou, et al.; Cell 90, 405 (1997) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-216 Revised 24-Oct-08
Monoclonal Antibody to HIF-1α (mgc3)
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SYNONYMS anti-Hypoxia-inducible Factor 1α MAb (mgc3)
anti-ARNT-interacting Protein MAb (mgc3)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Hypoxia-inducible Factor [HIF] / Related Products
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ALX-804-216-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
Monkey
CLONE: mgc3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Human HIF-1α (hypoxia-inducible factor 1α) (aa 530-826).
SPECIFICITY: Recognizes human, mouse, monkey, bovine and pig HIF-1α. Detects a band of ~116kDa by Western blot after hypoxic induction. Does not cross-react with HIF-1β or HIF-2α.
APPLICATION: Gel Supershift Assay (mouse HIF-1α only)
Immunocytochemistry (1:200)
Immunoprecipitation
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
[1] General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobuilins raised against thy hypoxia-inducible factor 1alpha: G. Camenisch, et al.; FASEB J. 13, 81 (1999) Abstract; Full Text
[2] Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell. Sci. 112, 1203 (1999) Abstract; Full Text
General Information
Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1α (HIF-1α) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1α rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1β.
Further Categories Containing This Product:
Aryl Hydrocarbon Receptor / Related Products • Monoclonal Antibodies
 
 
ALX-804-245 Revised 19-May-08
Monoclonal Antibody to Cytochrome c (denatured) (7H8.2C12)
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SYNONYMS anti-Cyt c (denatured) MAb (7H8.2C12)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytochrome c
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ALX-804-245-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Others
CLONE: 7H8.2C12
ISOTYPE: Mouse IgG2b
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity-purified.
FORMULATION: Liquid. In PBS containing 0.5% BSA and 0.05% sodium azide.
IMMUNOGEN: Non-native horse cytochrome c.
SPECIFICITY: Recognizes denatured cytochrome c from human, mouse, rat, pig, hamster, pigeon, horse, Drosophila and other species. Recognizes total cytochrome C which includes both apocytochrome c (in the cytosol without heme attached) and holocytochrome c (in the mitochondria with heme attached). Detects a band of ~15kDa by Western blot.
APPLICATION: Western Blot (1:500-1:1’000).
For immunocytochemistry, immunoprecipitation and functional application use MAb to Cytochrome c (native) (Blocking) (6H2.B4) (Prod. No. ALX-804-295).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Specific Literature References
A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells: R. Jemmerson, et al.; Eur. J. Immunol. 21, 143 (1991) Abstract
Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells: C.J. Narvaez and J. Welsh; J. Biol. Chem. 276, 9101 (2001) Abstract; Full Text
Mifepristone pretreatment overcomes resistance of prostate cancer cells to tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL): M.A. Eid, et al.; Mol. Cancer Ther. 1, 831 (2002) Abstract
Release of intact, monomeric cytochrome c from apoptotic and necrotic cells: R. Jemmerson, et al.; Cell Death Differ. 9, 538 (2002) Abstract
Styrylpyrone Derivative (SPD) induces apoptosis in a caspase-7-dependent manner in the human breast cancer cell line MCF-7: A.T. Lee, et al.; Cancer Cel Int. 3, 16 (2003) Abstract; Full Text
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis: C.Y. Liu, et al.; Ann. NY Acad. Sci. 1011, 133 (2004) Abstract
Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli: Y. Liang, et al.; Cell. Signal. 17, 243 (2005 17, 243 (2005) Abstract
Further Categories Containing This Product:
Mitochondria-based Apoptosis Detection • Monoclonal Antibodies
 
 
ALX-804-248 Revised 01-Oct-07
Monoclonal Antibody to TRAF2 (human) (33A1293)
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SYNONYMS anti-Tumor Necrosis Factor (TNF) Receptor-associated Factor 2 (human) (33A1293)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TRAFs / Related Products
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ALX-804-248-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 33A1293
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human TRAF2 (tumor necrosis factor (TNF) receptor-associated factor 2) (aa 205-222).
SPECIFICITY: Recognizes human TRAF2. Detects a band of ~57kDa by Western blot.
APPLICATION: Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Images
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Product Specific Literature References
Release of full-length 55-kDa TNF receptor 1 in exosome-like vesicles: a mechanism for generation of soluble cytokine receptors: F.I. Hawari, et al.; PNAS 101, 1297 (2004) Abstract; Full Text
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Monoclonal Antibodies • Autophagy Other Products
 
 
ALX-804-265 Revised 11-Mar-08
Monoclonal Antibody to MnSOD (human) (MnS-1)
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SYNONYMS anti-Manganese Superoxide Dismutase (human) MAb (MnS-1)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Superoxide Dismutases [SODs] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-265-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: MnS-1
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Human MnSOD (manganese superoxide dismutase).
SPECIFICITY: Recognizes human MnSOD.
APPLICATION: Immunohistochemistry (frozen sections): 0.5-10µg/ml. Use to stain both indirectly or directly acetone-fixed cryostat sections or cell smears; recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedure or the three stage immunoperoxidase technique on acetone-fixed cryostat sections.
Western Blot.
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-295 Revised 21-Sep-05
Monoclonal Antibody to Cytochrome c (native) (blocking) (6H2.B4)
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SYNONYMS anti-Cyt c (native) MAb (blocking) (6H2.B4)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytochrome c
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-295-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 6H2.B4
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Tissue-derived mouse cytochrome c.
SPECIFICITY: Recognizes native human, mouse and rat cytochrome c and immunoprecipitates the native protein of ~15kDa. Does not cross-react with denatured cytochrome c.
APPLICATION: Immunocytochemistry (see [4,6])
Immunohistochemistry (frozen section (2-4µg/ml))
Immunoprecipitation (2µg/106 cells)
Functional Application (blocks pro-apoptotic effect of cytosolic cytochrome c)
Note: Not recommended for Western blot. For Western blot use MAb to Cytochrome c (denatured) (7H8.2C12) (Prod. No. ALX-804-245).
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Product Specific Literature References
[1] Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c: S.C. Goshorn, et al.; J. Biol. Chem. 266, 2134 (1991) Abstract; Full Text
[2] Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c: X. Liu, et al.; Cell 86, 147 (1996) Abstract
[3] XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage: R. Datta, et al.; J. Biol. Chem. 275, 31733 (2000) Abstract; Full Text
[4] Activation of calpain I converts excitotoxic neuron death into a caspase-independent cell death: S. Lankiewicz, et al.; J. Biol. Chem. 275, 17064 (2000) Abstract; Full Text
[5] Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors: M. Maccarrone, et al.; J. Biol. Chem. 275, 31938 (2000) Abstract; Full Text
[6] Abrin triggers cell death by inactivating a thiol-specific antioxidant protein: S.F. Shih, et al.; J. Biol. Chem. 276, 21780 (2001) Abstract; Full Text
[7] Quantitation of mitochondrial alterations associated with apoptosis: M. Castedo, et al.; J. Immunol. Meth. 265, 39 (2002) Abstract
[8] Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells: W.H. Chan, et al.; J. Cell Biochem. 90, 327 (2003) Abstract
[9] Anti-apoptotic effects of curcumin on photosensitized human epidermal carcinoma A431 cells: W.H. Chan and H.J. Wu; J. Cell Biochem. 92, 200 (2004) Abstract
[10] Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispensable with ectopic expression of Smac or downregulation of Bcl-XL: R. Rashmi, et al.; Carcinogenesis 26, 713 (2005) Abstract
Further Categories Containing This Product:
Mitochondria-based Apoptosis Detection • Monoclonal Antibodies
 
 
ALX-804-381 Revised 16-Jun-08
Monoclonal Antibody to ATF6 (70B1413.1)
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SYNONYMS anti-Activating Transcription Factor 6 MAb (70B1413.1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products