ALEXIS Biochemicals: Monoclonal Antibody to Cytochrome c (native) (blocking) (6H2.B4)

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ALX-850-261

Revised 11-Apr-08

Cytochrome c (human) ELISA Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Cytochrome c

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-261-KI01		1 Kit	619.00 USD

Product Specification

SENSITIVITY:	0.05ng/ml (range 0.08 to 5ng/ml)
QUANTITY:	96 wells (~80 tests).
APPLICATION:	For the quantitative determination of human cytochrome c in cell culture lysates, serum and other body fluids.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Description

Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps of apoptosis initiation in cells. Cytochrome c release in the cytosol occurs prior to the activation of caspases and DNA fragmentation which is considered the hallmark of apoptosis.

General Information

Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps for initiating apoptosis in cells. Cytochrome c release in the cytosol occurs prior to the activation of caspases and DNA fragmentation which is considered the hallmark of apoptosis.
MANUFACTURER	Manufactured by Bender MedSystems.

Further Categories Containing This Product:

Mitochondria-based Apoptosis Detection • ELISA & EIA Kits

ALX-850-277

Revised 03-Feb-05

Cytochrome c Releasing Apoptosis Assay Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Cytochrome c

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-277-KI01		1 Kit	539.00 USD

Product Specification

QUANTITY:	100 tests.
KIT/SET CONTAINS:	10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 110µl DTT (1M), 1 vial 500X Protease Inhibitor Cocktail, 0.5ml Cytochrome c Antibody (0.2mg/ml).
APPLICATION:	For the detection of cytochrome c translocation from mitochondria into cytosol during apoptosis.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

Product Description

The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol. Cytochrome c released from mitochondria into cytosol is determined by Western blot using the cytochrome c antibody provided in the kit. The procedure is simple, straightforward and requires no ultracentrifugation.

General Information

MANUFACTURER

Manufactured by BioVision.

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Mitochondria-based Apoptosis Detection • Detection Kits

ALX-620-065

Revised 07-Mar-07

JC-1 . chloride
SYNONYMS	5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine chloride
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Mitochondria-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-065-M005		5 mg	198.00 USD

Product Specification

FORMULA:	C₂₅H₂₇Cl₅N₄
MW:	561.5
PURITY:	≥97% (HPLC)
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light, especially when in solution.

Product Description

For product description and literature references, see JC-1 . iodide (Prod. No. ALX-620-053).

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Dyes / Stains / Fluorescent Probes / Fluorescent Labels

ALX-620-053

Revised 16-Feb-05

JC-1 . iodide
SYNONYMS	5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Mitochondria-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-053-M005		5 mg	198.00 USD

Product Specification

FORMULA:	C₂₅H₂₇Cl₄IN₄
MW:	652.2
CAS NUMBER:	47729-63-5
PURITY:	≥98% (HPLC)
APPEARANCE:	Red solid.
SOLUBILITY:	Soluble in DMSO or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Keep cool and dry. Protect from light, especially when in solution.

Product Description

JC-1 is a very sensitive fluorescent probe to test Pgp activity. In acute myeloid leukemia (AML) samples JC-1 is capable of distinguishing between resistant, intermediate and sensitve groups, whereas rhodamine 123 only recognizes the resistent group. As in cell lines, the red emission band (597nm) of JC-1 appears to be more convenient for detection of low-level resistance in AML than other probes, such as rhodamine 123 (529nm) or calcein-AM (517nm).

Green fluorescent JC-1 existing as a monomer (λ_em=527nm) at low concentrations or at low membrane potential. However, at higher concentrations (aqueous solutions above 0.1µM) or higher potentials, JC-1 forms red fluorescent 'J-aggregates', which exhibit a broad excitation spectrum and a very narrow emission spectrum (λ_em=590nm). JC-1 stains mitochondria in living cells in a membrane potential-dependent function. JC-1 has been used in a functional assay of multidrug resistance cells and in apoptosis related mitochondrial modifications.

Product Specific Literature References

Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1: S.T. Smiley, et al.; PNAS 88, 3671 (1991) Abstract
J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential: M. Reers, et al.; Biochemistry 30, 4480 (1991) Abstract
A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1): A. Cossarizza, et al.; BBRC 197, 40 (1993) Abstract
Mitochondrial mass and membrane potential in coelomocytes from the earthworm Eisenia foetida: studies with fluorescent probes in single intact cells: A. Cossarizza, et al.; BBRC 214, 503 (1995) Abstract
Mitochondrial membrane potential monitored by JC-1 dye: M. Reers, et al.; Methods Enzymol. 260, 406 (1995) Abstract
Functional assay of multidrug resistant cells using JC-1, a carbocyanine fluorescent probe: J.M. Kühnel, et al.; Leukemia 11, 1147 (1997) Abstract
JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis: S. Salvioli, et al.; FEBS Lett. 411, 77 (1997) Abstract
Mitochondria and apoptosis: D.R. Green & J.C. Reed; Science 281, 1309 (1998) Abstract
Flow cytometric measurement of mitochondrial mass and function: a novel method for assessing chemoresistance: M. Mancini, et al.; Ann. Surg. Oncol. 5, 287 (1998) Abstract
Flavonoid-related modulators of multidrug resistance: synthesis, pharmacological activity, and structure-activity relationships: J. Ferte, et al.; J. Med. Chem. 42, 478 (1999) Abstract
UVA1 radiation triggers two different final apoptotic pathways: D.E. Godar; J. Invest. Dermatol. 112, 3 (1999) Abstract
A rapid method for the evaluation of compounds with mitochondria-protective properties: R. Nuydens, et al.; J. Neurosci. Methods 92, 153 (1999) Abstract
JC-1: a very sensitive fluorescent probe to test Pgp activity in adult acute myeloid leukemia: O. Legrand, et al.; Blood 97, 502 (2001) Abstract; Full Text
Intra- and intercellular distribution of mitochondrial probes and changes after treatment with MDR modulators: G. Diaz, et al.; IUBMB Life 51, 121 (2001) Abstract

Further Categories Containing This Product:

MPTP [Mitochondrial Transition Pore] / Related Products • Dyes / Stains / Fluorescent Probes / Fluorescent Labels

ALX-850-232

Revised 22-Apr-08

MitoCapture™ Mitochondrial Apoptosis Detection Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Mitochondria-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-232-KI01		25 tests	175.00 USD
ALX-850-232-KI02		100 tests	385.00 USD

Product Specification

KIT/SET CONTAINS:	MitoCapture™ Reagent Incubation Buffer.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Store Incubation Buffer at 4°C after opening. Avoid freeze-thaw cycles. Protect from light.

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Product Description

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCapture™ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial membrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy and in apoptotic cells. In healthy cells, MitoCapture™ accumulates and aggregates in the mitochondria, giving them a bright red fluorescence. In apoptotic cells, MitoCapture™ cannot aggregate in the mitochondria due to the altered mitochondrial membrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using the FITC channel for green monomers (Ex(max): 488nm; Em(max): 530nm + 30nm), and the PI channel for red aggregates (Ex(max): 488nm; Em(max): 590nm + 42nm). An assay is completed within 20-30 min.

Product Specific Literature References

TANK2, a new TRF1-associated poly(ADP-ribose) polymerase, causes rapid induction of cell death upon overexpression: P.G. Kaminker, et al.; J. Biol. Chem. 276, 35891 (2001) Abstract; Full Text

General Information

MANUFACTURER

Manufactured by BioVision.

Further Categories Containing This Product:

Detection Kits

ALX-804-245

Revised 19-May-08

Monoclonal Antibody to Cytochrome c (denatured) (7H8.2C12)
SYNONYMS	anti-Cyt c (denatured) MAb (7H8.2C12)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Cytochrome c

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-245-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Pig Others
CLONE:	7H8.2C12
ISOTYPE:	Mouse IgG2b
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity-purified.
FORMULATION:	Liquid. In PBS containing 0.5% BSA and 0.05% sodium azide.
IMMUNOGEN:	Non-native horse cytochrome c.
SPECIFICITY:	Recognizes denatured cytochrome c from human, mouse, rat, pig, hamster, pigeon, horse, Drosophila and other species. Recognizes total cytochrome C which includes both apocytochrome c (in the cytosol without heme attached) and holocytochrome c (in the mitochondria with heme attached). Detects a band of ~15kDa by Western blot.
APPLICATION:	Western Blot (1:500-1:1’000). For immunocytochemistry, immunoprecipitation and functional application use MAb to Cytochrome c (native) (Blocking) (6H2.B4) (Prod. No. ALX-804-295).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.

Product Specific Literature References

A monoclonal antibody specific for a cytochrome c T cell stimulatory peptide inhibits T cell responses and affects the way the peptide associates with antigen-presenting cells: R. Jemmerson, et al.; Eur. J. Immunol. 21, 143 (1991) Abstract
Role of mitochondria and caspases in vitamin D-mediated apoptosis of MCF-7 breast cancer cells: C.J. Narvaez and J. Welsh; J. Biol. Chem. 276, 9101 (2001) Abstract; Full Text
Mifepristone pretreatment overcomes resistance of prostate cancer cells to tumor necrosis factor alpha-related apoptosis-inducing ligand (TRAIL): M.A. Eid, et al.; Mol. Cancer Ther. 1, 831 (2002) Abstract
Release of intact, monomeric cytochrome c from apoptotic and necrotic cells: R. Jemmerson, et al.; Cell Death Differ. 9, 538 (2002) Abstract
Styrylpyrone Derivative (SPD) induces apoptosis in a caspase-7-dependent manner in the human breast cancer cell line MCF-7: A.T. Lee, et al.; Cancer Cel Int. 3, 16 (2003) Abstract; Full Text
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis: C.Y. Liu, et al.; Ann. NY Acad. Sci. 1011, 133 (2004) Abstract
Mitochondria from TRAIL-resistant prostate cancer cells are capable of responding to apoptotic stimuli: Y. Liang, et al.; Cell. Signal. 17, 243 (2005 17, 243 (2005) Abstract

Further Categories Containing This Product:

Mitochondria-based Apoptosis Detection • Monoclonal Antibodies

ALX-804-295

Revised 21-Sep-05

Monoclonal Antibody to Cytochrome c (native) (blocking) (6H2.B4)
SYNONYMS	anti-Cyt c (native) MAb (blocking) (6H2.B4)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Cytochrome c

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-295-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
CLONE:	6H2.B4
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:	Tissue-derived mouse cytochrome c.
SPECIFICITY:	Recognizes native human, mouse and rat cytochrome c and immunoprecipitates the native protein of ~15kDa. Does not cross-react with denatured cytochrome c.
APPLICATION:	Immunocytochemistry (see [4,6]) Immunohistochemistry (frozen section (2-4µg/ml)) Immunoprecipitation (2µg/10⁶ cells) Functional Application (blocks pro-apoptotic effect of cytosolic cytochrome c) Note: Not recommended for Western blot. For Western blot use MAb to Cytochrome c (denatured) (7H8.2C12) (Prod. No. ALX-804-245).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months when stored at +4°C.

Product Specific Literature References

[1] Common structural features among monoclonal antibodies binding the same antigenic region of cytochrome c: S.C. Goshorn, et al.; J. Biol. Chem. 266, 2134 (1991) Abstract; Full Text
[2] Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c: X. Liu, et al.; Cell 86, 147 (1996) Abstract
[3] XIAP regulates DNA damage-induced apoptosis downstream of caspase-9 cleavage: R. Datta, et al.; J. Biol. Chem. 275, 31733 (2000) Abstract; Full Text
[4] Activation of calpain I converts excitotoxic neuron death into a caspase-independent cell death: S. Lankiewicz, et al.; J. Biol. Chem. 275, 17064 (2000) Abstract; Full Text
[5] Anandamide induces apoptosis in human cells via vanilloid receptors. Evidence for a protective role of cannabinoid receptors: M. Maccarrone, et al.; J. Biol. Chem. 275, 31938 (2000) Abstract; Full Text
[6] Abrin triggers cell death by inactivating a thiol-specific antioxidant protein: S.F. Shih, et al.; J. Biol. Chem. 276, 21780 (2001) Abstract; Full Text
[7] Quantitation of mitochondrial alterations associated with apoptosis: M. Castedo, et al.; J. Immunol. Meth. 265, 39 (2002) Abstract
[8] Curcumin inhibits UV irradiation-induced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells: W.H. Chan, et al.; J. Cell Biochem. 90, 327 (2003) Abstract
[9] Anti-apoptotic effects of curcumin on photosensitized human epidermal carcinoma A431 cells: W.H. Chan and H.J. Wu; J. Cell Biochem. 92, 200 (2004) Abstract
[10] Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispensable with ectopic expression of Smac or downregulation of Bcl-XL: R. Rashmi, et al.; Carcinogenesis 26, 713 (2005) Abstract

Further Categories Containing This Product:

Mitochondria-based Apoptosis Detection • Monoclonal Antibodies

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